Strain Fitness in Escherichia coli ECOR38 around HEPCGN_22150

Experiment: Control_ECOR38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenF and menD are separated by 88 nucleotidesmenD and menH overlap by 4 nucleotidesmenH and menB are separated by 14 nucleotides HEPCGN_22145: menF - isochorismate synthase MenF, at 3,187,309 to 3,188,604 menF HEPCGN_22150: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene- 1-carboxylic-acid synthase, at 3,188,693 to 3,190,363 menD HEPCGN_22155: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 3,190,360 to 3,191,118 menH HEPCGN_22160: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 3,191,133 to 3,191,990 menB Position (kb) 3188 3189 3190 3191Strain fitness (log2 ratio) -1 0 1 2at 3187.722 kb on + strand, within menFat 3187.759 kb on - strand, within menFat 3187.781 kb on + strand, within menFat 3187.833 kb on - strand, within menFat 3187.982 kb on - strand, within menFat 3188.103 kb on + strand, within menFat 3188.225 kb on + strand, within menFat 3188.226 kb on - strand, within menFat 3188.274 kb on + strand, within menFat 3188.376 kb on + strand, within menFat 3188.377 kb on - strand, within menFat 3188.438 kb on + strand, within menFat 3188.502 kb on - strandat 3188.583 kb on + strandat 3188.583 kb on + strandat 3188.606 kb on - strandat 3188.621 kb on + strandat 3188.637 kb on + strandat 3188.642 kb on + strandat 3188.650 kb on + strandat 3188.659 kb on - strandat 3188.664 kb on + strandat 3189.092 kb on - strand, within menDat 3189.669 kb on + strand, within menDat 3189.859 kb on + strand, within menDat 3189.874 kb on - strand, within menDat 3190.056 kb on + strand, within menDat 3190.057 kb on - strand, within menDat 3190.057 kb on - strand, within menDat 3190.157 kb on + strand, within menDat 3190.158 kb on - strand, within menDat 3190.215 kb on - strandat 3190.560 kb on - strand, within menHat 3190.567 kb on - strand, within menHat 3190.583 kb on - strand, within menHat 3190.788 kb on - strand, within menHat 3190.966 kb on + strand, within menHat 3190.966 kb on + strand, within menHat 3190.970 kb on - strand, within menHat 3191.055 kb on + strandat 3191.055 kb on + strandat 3191.132 kb on + strandat 3191.211 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECOR38
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3,187,722 + menF HEPCGN_22145 0.32 +0.4
3,187,759 - menF HEPCGN_22145 0.35 +0.3
3,187,781 + menF HEPCGN_22145 0.36 +0.6
3,187,833 - menF HEPCGN_22145 0.40 +0.2
3,187,982 - menF HEPCGN_22145 0.52 +1.4
3,188,103 + menF HEPCGN_22145 0.61 +0.5
3,188,225 + menF HEPCGN_22145 0.71 +0.4
3,188,226 - menF HEPCGN_22145 0.71 -0.5
3,188,274 + menF HEPCGN_22145 0.74 +0.7
3,188,376 + menF HEPCGN_22145 0.82 +0.4
3,188,377 - menF HEPCGN_22145 0.82 +0.6
3,188,438 + menF HEPCGN_22145 0.87 +0.2
3,188,502 - +0.1
3,188,583 + -0.8
3,188,583 + +0.6
3,188,606 - -0.2
3,188,621 + +0.1
3,188,637 + -0.3
3,188,642 + +0.3
3,188,650 + +0.6
3,188,659 - -0.1
3,188,664 + +0.1
3,189,092 - menD HEPCGN_22150 0.24 -0.0
3,189,669 + menD HEPCGN_22150 0.58 +0.1
3,189,859 + menD HEPCGN_22150 0.70 +1.8
3,189,874 - menD HEPCGN_22150 0.71 -0.2
3,190,056 + menD HEPCGN_22150 0.82 +1.2
3,190,057 - menD HEPCGN_22150 0.82 +0.3
3,190,057 - menD HEPCGN_22150 0.82 -0.2
3,190,157 + menD HEPCGN_22150 0.88 -0.1
3,190,158 - menD HEPCGN_22150 0.88 +1.4
3,190,215 - -0.5
3,190,560 - menH HEPCGN_22155 0.26 +0.1
3,190,567 - menH HEPCGN_22155 0.27 +0.9
3,190,583 - menH HEPCGN_22155 0.29 -0.7
3,190,788 - menH HEPCGN_22155 0.56 +0.4
3,190,966 + menH HEPCGN_22155 0.80 +0.0
3,190,966 + menH HEPCGN_22155 0.80 +0.9
3,190,970 - menH HEPCGN_22155 0.80 +0.2
3,191,055 + +0.3
3,191,055 + +0.6
3,191,132 + -0.6
3,191,211 + -1.0

Or see this region's nucleotide sequence