Experiment: Control_ECOR38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metI and metQ are separated by 39 nucleotides metQ and rcsF are separated by 117 nucleotides rcsF and tsaA overlap by 4 nucleotides tsaA and proS are separated by 110 nucleotides
HEPCGN_07995: metI - D-methionine transport system permease protein MetI, at 335,342 to 335,995
metI
HEPCGN_08000: metQ - methionine ABC transporter substrate-binding lipoprotein MetQ, at 336,035 to 336,850
metQ
HEPCGN_08005: rcsF - Rcs stress response system protein RcsF, at 336,968 to 337,372
rcsF
HEPCGN_08010: tsaA - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 337,369 to 338,076
tsaA
HEPCGN_08015: proS - proline--tRNA ligase, at 338,187 to 339,905
proS
Position (kb) 336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 335.993 kb on + strand at 335.994 kb on - strand at 336.010 kb on + strand at 336.029 kb on + strand at 336.030 kb on - strand at 336.134 kb on - strand, within metQ at 336.134 kb on - strand, within metQ at 336.204 kb on - strand, within metQ at 336.239 kb on + strand, within metQ at 336.298 kb on + strand, within metQ at 336.299 kb on - strand, within metQ at 336.299 kb on - strand, within metQ at 336.299 kb on - strand, within metQ at 336.383 kb on + strand, within metQ at 336.647 kb on + strand, within metQ at 336.647 kb on + strand, within metQ at 336.647 kb on + strand, within metQ at 336.647 kb on + strand, within metQ at 336.648 kb on - strand, within metQ at 336.648 kb on - strand, within metQ at 336.648 kb on - strand, within metQ at 336.648 kb on - strand, within metQ at 336.686 kb on - strand, within metQ at 336.700 kb on + strand, within metQ at 336.701 kb on - strand, within metQ at 336.782 kb on + strand at 336.823 kb on + strand at 336.824 kb on - strand at 336.851 kb on + strand at 336.856 kb on - strand at 336.879 kb on - strand at 336.935 kb on - strand at 336.965 kb on - strand at 336.967 kb on + strand at 336.968 kb on - strand at 336.968 kb on - strand at 336.994 kb on - strand at 337.024 kb on - strand, within rcsF at 337.121 kb on + strand, within rcsF at 337.234 kb on + strand, within rcsF at 337.278 kb on + strand, within rcsF at 337.282 kb on - strand, within rcsF at 337.288 kb on + strand, within rcsF at 337.289 kb on - strand, within rcsF at 337.310 kb on - strand, within rcsF at 337.336 kb on - strand at 337.336 kb on - strand at 337.402 kb on - strand at 337.414 kb on + strand at 337.455 kb on - strand, within tsaA at 337.475 kb on + strand, within tsaA at 337.475 kb on + strand, within tsaA at 337.476 kb on - strand, within tsaA at 337.493 kb on - strand, within tsaA at 337.493 kb on - strand, within tsaA at 337.538 kb on + strand, within tsaA at 337.538 kb on + strand, within tsaA at 337.671 kb on + strand, within tsaA at 337.671 kb on + strand, within tsaA at 337.672 kb on - strand, within tsaA at 337.672 kb on - strand, within tsaA at 337.755 kb on + strand, within tsaA at 337.780 kb on + strand, within tsaA at 337.781 kb on - strand, within tsaA at 337.781 kb on - strand, within tsaA at 337.822 kb on + strand, within tsaA at 337.822 kb on + strand, within tsaA at 337.823 kb on - strand, within tsaA at 337.917 kb on - strand, within tsaA at 337.917 kb on - strand, within tsaA at 337.937 kb on + strand, within tsaA at 337.968 kb on + strand, within tsaA at 337.969 kb on - strand, within tsaA at 337.969 kb on - strand, within tsaA at 337.969 kb on - strand, within tsaA at 338.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECOR38 remove 335,993 + -0.8 335,994 - +0.2 336,010 + -1.6 336,029 + -0.1 336,030 - +0.6 336,134 - metQ HEPCGN_08000 0.12 +0.1 336,134 - metQ HEPCGN_08000 0.12 -0.4 336,204 - metQ HEPCGN_08000 0.21 -0.1 336,239 + metQ HEPCGN_08000 0.25 -0.4 336,298 + metQ HEPCGN_08000 0.32 +0.2 336,299 - metQ HEPCGN_08000 0.32 +0.3 336,299 - metQ HEPCGN_08000 0.32 +0.9 336,299 - metQ HEPCGN_08000 0.32 +0.2 336,383 + metQ HEPCGN_08000 0.43 -0.1 336,647 + metQ HEPCGN_08000 0.75 +0.5 336,647 + metQ HEPCGN_08000 0.75 +0.4 336,647 + metQ HEPCGN_08000 0.75 -0.7 336,647 + metQ HEPCGN_08000 0.75 -0.5 336,648 - metQ HEPCGN_08000 0.75 -0.3 336,648 - metQ HEPCGN_08000 0.75 -1.1 336,648 - metQ HEPCGN_08000 0.75 -0.2 336,648 - metQ HEPCGN_08000 0.75 -0.5 336,686 - metQ HEPCGN_08000 0.80 +1.1 336,700 + metQ HEPCGN_08000 0.81 -0.6 336,701 - metQ HEPCGN_08000 0.82 -0.1 336,782 + -0.6 336,823 + -0.7 336,824 - +0.2 336,851 + -3.5 336,856 - +0.3 336,879 - +0.3 336,935 - -0.4 336,965 - -0.4 336,967 + +0.5 336,968 - +0.4 336,968 - -0.1 336,994 - -0.2 337,024 - rcsF HEPCGN_08005 0.14 +0.1 337,121 + rcsF HEPCGN_08005 0.38 -1.2 337,234 + rcsF HEPCGN_08005 0.66 -0.2 337,278 + rcsF HEPCGN_08005 0.77 -0.2 337,282 - rcsF HEPCGN_08005 0.78 -0.0 337,288 + rcsF HEPCGN_08005 0.79 +0.1 337,289 - rcsF HEPCGN_08005 0.79 +0.2 337,310 - rcsF HEPCGN_08005 0.84 +0.1 337,336 - -0.1 337,336 - -0.1 337,402 - -0.2 337,414 + +0.3 337,455 - tsaA HEPCGN_08010 0.12 -1.2 337,475 + tsaA HEPCGN_08010 0.15 +0.4 337,475 + tsaA HEPCGN_08010 0.15 -0.3 337,476 - tsaA HEPCGN_08010 0.15 +0.5 337,493 - tsaA HEPCGN_08010 0.18 +1.1 337,493 - tsaA HEPCGN_08010 0.18 +0.2 337,538 + tsaA HEPCGN_08010 0.24 -0.3 337,538 + tsaA HEPCGN_08010 0.24 +0.1 337,671 + tsaA HEPCGN_08010 0.43 +0.1 337,671 + tsaA HEPCGN_08010 0.43 -0.1 337,672 - tsaA HEPCGN_08010 0.43 +0.4 337,672 - tsaA HEPCGN_08010 0.43 +0.1 337,755 + tsaA HEPCGN_08010 0.55 +0.2 337,780 + tsaA HEPCGN_08010 0.58 -0.6 337,781 - tsaA HEPCGN_08010 0.58 -0.4 337,781 - tsaA HEPCGN_08010 0.58 +0.0 337,822 + tsaA HEPCGN_08010 0.64 -0.3 337,822 + tsaA HEPCGN_08010 0.64 +0.1 337,823 - tsaA HEPCGN_08010 0.64 -0.7 337,917 - tsaA HEPCGN_08010 0.77 +0.0 337,917 - tsaA HEPCGN_08010 0.77 +1.4 337,937 + tsaA HEPCGN_08010 0.80 -0.1 337,968 + tsaA HEPCGN_08010 0.85 +0.5 337,969 - tsaA HEPCGN_08010 0.85 +0.2 337,969 - tsaA HEPCGN_08010 0.85 +0.8 337,969 - tsaA HEPCGN_08010 0.85 +0.3 338,017 - -1.1
Or see this region's nucleotide sequence