Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yubM and yubL are separated by 65 nucleotides yubL and ssb are separated by 56 nucleotides ssb and NIAGMN_28825 are separated by 594 nucleotides NIAGMN_28825 and rsmA overlap by 1 nucleotides
NIAGMN_28810: yubM - Uncharacterized protein YubM, at 69,265 to 71,223
yubM
NIAGMN_28815: yubL - UPF0401 protein YubL, at 71,289 to 71,522
yubL
NIAGMN_28820: ssb - single-stranded DNA-binding protein, at 71,579 to 72,031
ssb
NIAGMN_28825: NIAGMN_28825 - hypothetical protein, at 72,626 to 72,880
_28825
NIAGMN_28830: rsmA - SAM-dependent methyltransferase, at 72,880 to 73,443
rsmA
Position (kb) 71
72
73 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 70.730 kb on + strand, within yubM at 70.731 kb on - strand, within yubM at 70.815 kb on + strand, within yubM at 70.815 kb on + strand, within yubM at 70.815 kb on + strand, within yubM at 70.816 kb on - strand, within yubM at 71.006 kb on - strand, within yubM at 71.022 kb on + strand, within yubM at 71.023 kb on - strand, within yubM at 71.091 kb on + strand at 71.091 kb on + strand at 71.091 kb on + strand at 71.091 kb on + strand at 71.092 kb on - strand at 71.092 kb on - strand at 71.176 kb on - strand at 71.187 kb on + strand at 71.187 kb on + strand at 71.187 kb on + strand at 71.187 kb on + strand at 71.205 kb on + strand at 71.214 kb on + strand at 71.214 kb on + strand at 71.215 kb on - strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.223 kb on + strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.224 kb on - strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.227 kb on + strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.228 kb on - strand at 71.230 kb on + strand at 71.230 kb on + strand at 71.230 kb on + strand at 71.230 kb on + strand at 71.230 kb on + strand at 71.230 kb on + strand at 71.231 kb on - strand at 71.231 kb on - strand at 71.231 kb on - strand at 71.231 kb on - strand at 71.245 kb on + strand at 71.248 kb on + strand at 71.248 kb on + strand at 71.248 kb on + strand at 71.248 kb on + strand at 71.248 kb on + strand at 71.249 kb on - strand at 71.249 kb on - strand at 71.291 kb on - strand at 71.294 kb on + strand at 71.294 kb on + strand at 71.294 kb on + strand at 71.294 kb on + strand at 71.295 kb on - strand at 71.295 kb on - strand at 71.295 kb on - strand at 71.295 kb on - strand at 71.295 kb on - strand at 71.297 kb on + strand at 71.298 kb on - strand at 71.382 kb on - strand, within yubL at 71.382 kb on - strand, within yubL at 71.474 kb on + strand, within yubL at 71.474 kb on + strand, within yubL at 71.502 kb on + strand at 71.503 kb on - strand at 71.591 kb on - strand at 71.693 kb on + strand, within ssb at 71.693 kb on + strand, within ssb at 71.694 kb on - strand, within ssb at 71.722 kb on + strand, within ssb at 71.723 kb on - strand, within ssb at 71.723 kb on - strand, within ssb at 71.723 kb on - strand, within ssb at 71.743 kb on - strand, within ssb at 71.752 kb on + strand, within ssb at 71.753 kb on - strand, within ssb at 71.796 kb on + strand, within ssb at 71.796 kb on + strand, within ssb at 71.797 kb on - strand, within ssb at 71.821 kb on - strand, within ssb at 71.821 kb on - strand, within ssb at 71.821 kb on - strand, within ssb at 71.964 kb on + strand, within ssb at 71.964 kb on + strand, within ssb at 71.964 kb on + strand, within ssb at 71.964 kb on + strand, within ssb at 71.965 kb on - strand, within ssb at 71.996 kb on + strand at 72.014 kb on - strand at 72.134 kb on + strand at 72.323 kb on - strand at 72.596 kb on + strand at 72.596 kb on + strand at 72.596 kb on + strand at 72.596 kb on + strand at 72.623 kb on + strand at 72.623 kb on + strand at 72.623 kb on + strand at 72.623 kb on + strand at 72.623 kb on + strand at 72.624 kb on - strand at 72.624 kb on - strand at 72.653 kb on + strand, within NIAGMN_28825 at 72.653 kb on + strand, within NIAGMN_28825 at 72.653 kb on + strand, within NIAGMN_28825 at 72.653 kb on + strand, within NIAGMN_28825 at 72.654 kb on - strand, within NIAGMN_28825 at 72.660 kb on - strand, within NIAGMN_28825 at 72.660 kb on - strand, within NIAGMN_28825 at 72.665 kb on + strand, within NIAGMN_28825 at 72.665 kb on + strand, within NIAGMN_28825 at 72.665 kb on + strand, within NIAGMN_28825 at 72.666 kb on - strand, within NIAGMN_28825 at 72.666 kb on - strand, within NIAGMN_28825 at 72.671 kb on + strand, within NIAGMN_28825 at 72.671 kb on + strand, within NIAGMN_28825 at 72.672 kb on - strand, within NIAGMN_28825 at 72.678 kb on - strand, within NIAGMN_28825 at 72.683 kb on + strand, within NIAGMN_28825 at 72.683 kb on + strand, within NIAGMN_28825 at 72.684 kb on - strand, within NIAGMN_28825 at 72.690 kb on - strand, within NIAGMN_28825 at 72.690 kb on - strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.695 kb on + strand, within NIAGMN_28825 at 72.778 kb on + strand, within NIAGMN_28825 at 72.778 kb on + strand, within NIAGMN_28825 at 72.778 kb on + strand, within NIAGMN_28825 at 72.778 kb on + strand, within NIAGMN_28825 at 72.778 kb on + strand, within NIAGMN_28825 at 72.779 kb on - strand, within NIAGMN_28825 at 72.856 kb on + strand at 72.857 kb on - strand at 72.857 kb on - strand at 72.897 kb on + strand at 72.918 kb on + strand at 72.919 kb on - strand at 72.919 kb on - strand at 72.919 kb on - strand at 72.919 kb on - strand at 72.920 kb on + strand at 72.921 kb on - strand at 72.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 70,730 + yubM NIAGMN_28810 0.75 -0.4 70,731 - yubM NIAGMN_28810 0.75 -0.0 70,815 + yubM NIAGMN_28810 0.79 +2.7 70,815 + yubM NIAGMN_28810 0.79 -0.1 70,815 + yubM NIAGMN_28810 0.79 +0.4 70,816 - yubM NIAGMN_28810 0.79 -2.9 71,006 - yubM NIAGMN_28810 0.89 -1.3 71,022 + yubM NIAGMN_28810 0.90 -3.1 71,023 - yubM NIAGMN_28810 0.90 -1.1 71,091 + -2.7 71,091 + -1.9 71,091 + +0.2 71,091 + -2.1 71,092 - -2.6 71,092 - +1.2 71,176 - +0.0 71,187 + -0.3 71,187 + -0.7 71,187 + +2.5 71,187 + -1.6 71,205 + +1.4 71,214 + +1.5 71,214 + +3.1 71,215 - -4.2 71,223 + -0.7 71,223 + -1.7 71,223 + -0.1 71,223 + -0.4 71,223 + -0.1 71,223 + -2.5 71,223 + -3.7 71,223 + +0.9 71,223 + -3.1 71,223 + -3.8 71,224 - +2.8 71,224 - -2.9 71,224 - -0.1 71,224 - +1.7 71,224 - -1.8 71,224 - +1.1 71,224 - +1.8 71,227 + -0.1 71,227 + +3.1 71,227 + +0.9 71,227 + -0.3 71,227 + -0.3 71,227 + -1.6 71,227 + +1.2 71,227 + -1.4 71,227 + -0.0 71,227 + +0.2 71,227 + +0.3 71,227 + -0.4 71,227 + +0.5 71,227 + -0.1 71,227 + +0.5 71,227 + +0.1 71,228 - -1.8 71,228 - -1.8 71,228 - -2.3 71,228 - -2.5 71,228 - -2.1 71,228 - +1.1 71,228 - -1.3 71,228 - -0.5 71,228 - -0.8 71,228 - +0.3 71,228 - -3.4 71,228 - -2.5 71,228 - -2.6 71,228 - -0.4 71,230 + +0.8 71,230 + -1.6 71,230 + -0.9 71,230 + +2.1 71,230 + +1.8 71,230 + +0.3 71,231 - -3.2 71,231 - +2.7 71,231 - +0.3 71,231 - -2.7 71,245 + +0.8 71,248 + -1.9 71,248 + +0.6 71,248 + +1.6 71,248 + +0.7 71,248 + +0.8 71,249 - -1.2 71,249 - -0.0 71,291 - -0.9 71,294 + +3.3 71,294 + -0.7 71,294 + -1.3 71,294 + +0.1 71,295 - -1.4 71,295 - -2.3 71,295 - -0.2 71,295 - -1.2 71,295 - +2.4 71,297 + -3.3 71,298 - -1.1 71,382 - yubL NIAGMN_28815 0.40 +0.1 71,382 - yubL NIAGMN_28815 0.40 -1.1 71,474 + yubL NIAGMN_28815 0.79 -2.7 71,474 + yubL NIAGMN_28815 0.79 -1.8 71,502 + -2.2 71,503 - -1.4 71,591 - -0.2 71,693 + ssb NIAGMN_28820 0.25 +1.1 71,693 + ssb NIAGMN_28820 0.25 +1.2 71,694 - ssb NIAGMN_28820 0.25 -1.0 71,722 + ssb NIAGMN_28820 0.32 +1.2 71,723 - ssb NIAGMN_28820 0.32 +0.6 71,723 - ssb NIAGMN_28820 0.32 -2.0 71,723 - ssb NIAGMN_28820 0.32 -1.7 71,743 - ssb NIAGMN_28820 0.36 -2.7 71,752 + ssb NIAGMN_28820 0.38 +0.7 71,753 - ssb NIAGMN_28820 0.38 -1.0 71,796 + ssb NIAGMN_28820 0.48 -0.3 71,796 + ssb NIAGMN_28820 0.48 -2.6 71,797 - ssb NIAGMN_28820 0.48 -1.9 71,821 - ssb NIAGMN_28820 0.53 -1.9 71,821 - ssb NIAGMN_28820 0.53 +0.3 71,821 - ssb NIAGMN_28820 0.53 -0.4 71,964 + ssb NIAGMN_28820 0.85 -2.6 71,964 + ssb NIAGMN_28820 0.85 -1.8 71,964 + ssb NIAGMN_28820 0.85 -0.3 71,964 + ssb NIAGMN_28820 0.85 -0.4 71,965 - ssb NIAGMN_28820 0.85 -1.6 71,996 + +0.1 72,014 - +0.1 72,134 + +0.1 72,323 - -2.4 72,596 + +0.6 72,596 + -1.4 72,596 + +2.3 72,596 + -1.1 72,623 + -0.9 72,623 + +0.5 72,623 + +0.2 72,623 + +1.6 72,623 + -3.6 72,624 - -1.6 72,624 - -2.6 72,653 + NIAGMN_28825 0.11 -0.4 72,653 + NIAGMN_28825 0.11 -2.9 72,653 + NIAGMN_28825 0.11 +0.7 72,653 + NIAGMN_28825 0.11 -3.3 72,654 - NIAGMN_28825 0.11 +3.3 72,660 - NIAGMN_28825 0.13 +3.4 72,660 - NIAGMN_28825 0.13 +1.8 72,665 + NIAGMN_28825 0.15 +0.8 72,665 + NIAGMN_28825 0.15 +0.1 72,665 + NIAGMN_28825 0.15 +0.8 72,666 - NIAGMN_28825 0.16 -0.4 72,666 - NIAGMN_28825 0.16 +0.9 72,671 + NIAGMN_28825 0.18 -0.7 72,671 + NIAGMN_28825 0.18 +0.4 72,672 - NIAGMN_28825 0.18 -2.1 72,678 - NIAGMN_28825 0.20 -1.4 72,683 + NIAGMN_28825 0.22 -2.9 72,683 + NIAGMN_28825 0.22 +2.5 72,684 - NIAGMN_28825 0.23 +0.5 72,690 - NIAGMN_28825 0.25 -0.2 72,690 - NIAGMN_28825 0.25 -3.0 72,695 + NIAGMN_28825 0.27 +0.1 72,695 + NIAGMN_28825 0.27 +1.6 72,695 + NIAGMN_28825 0.27 -0.1 72,695 + NIAGMN_28825 0.27 -0.1 72,695 + NIAGMN_28825 0.27 +1.0 72,695 + NIAGMN_28825 0.27 +1.1 72,695 + NIAGMN_28825 0.27 +0.4 72,778 + NIAGMN_28825 0.60 +0.4 72,778 + NIAGMN_28825 0.60 +0.2 72,778 + NIAGMN_28825 0.60 -1.0 72,778 + NIAGMN_28825 0.60 +2.7 72,778 + NIAGMN_28825 0.60 +0.7 72,779 - NIAGMN_28825 0.60 -0.1 72,856 + -1.5 72,857 - -1.9 72,857 - -0.1 72,897 + -0.4 72,918 + +0.2 72,919 - +0.1 72,919 - +2.3 72,919 - +2.7 72,919 - -0.2 72,920 + +0.6 72,921 - +1.7 72,921 - -2.5
Or see this region's nucleotide sequence