Strain Fitness in Escherichia coli ECRC102 around NIAGMN_28710

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrepA and tap overlap by 8 nucleotidestap and repA2 are separated by 235 nucleotidesrepA2 and NIAGMN_28710 are separated by 239 nucleotidesNIAGMN_28710 and NIAGMN_28715 are separated by 37 nucleotidesNIAGMN_28715 and yncB are separated by 45 nucleotidesyncB and NIAGMN_28730 are separated by 201 nucleotides NIAGMN_28690: repA - incFII family plasmid replication initiator RepA, at 45,755 to 46,612 repA NIAGMN_28695: tap - RepA leader peptide Tap, at 46,605 to 46,679 tap NIAGMN_28705: repA2 - Replication regulatory protein repA2, at 46,915 to 47,169 repA2 NIAGMN_28710: NIAGMN_28710 - W0003, at 47,409 to 47,747 _28710 NIAGMN_28715: NIAGMN_28715 - transcriptional regulator, at 47,785 to 47,994 _28715 NIAGMN_28720: yncB - Uncharacterized endonuclease, at 48,040 to 48,501 yncB NIAGMN_28730: NIAGMN_28730 - hypothetical protein, at 48,703 to 48,954 _28730 Position (kb) 47 48Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 46.578 kb on - strandat 46.590 kb on + strandat 46.591 kb on - strandat 46.591 kb on - strandat 46.591 kb on - strandat 46.627 kb on - strand, within tapat 46.680 kb on - strandat 46.682 kb on - strandat 46.685 kb on + strandat 46.716 kb on + strandat 46.723 kb on + strandat 46.723 kb on + strandat 46.799 kb on - strandat 46.832 kb on - strandat 46.875 kb on - strandat 46.905 kb on - strandat 46.945 kb on + strand, within repA2at 47.014 kb on - strand, within repA2at 47.014 kb on - strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.015 kb on + strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.016 kb on - strand, within repA2at 47.038 kb on + strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.039 kb on - strand, within repA2at 47.062 kb on - strand, within repA2at 47.062 kb on - strand, within repA2at 47.117 kb on + strand, within repA2at 47.118 kb on - strand, within repA2at 47.118 kb on - strand, within repA2at 47.118 kb on - strand, within repA2at 47.183 kb on + strandat 47.187 kb on + strandat 47.187 kb on + strandat 47.187 kb on + strandat 47.188 kb on - strandat 47.188 kb on - strandat 47.188 kb on - strandat 47.213 kb on - strandat 47.213 kb on - strandat 47.213 kb on - strandat 47.215 kb on + strandat 47.216 kb on - strandat 47.287 kb on - strandat 47.287 kb on - strandat 47.297 kb on - strandat 47.306 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.318 kb on + strandat 47.319 kb on - strandat 47.319 kb on - strandat 47.321 kb on + strandat 47.322 kb on - strandat 47.334 kb on + strandat 47.335 kb on - strandat 47.414 kb on + strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.415 kb on - strandat 47.450 kb on + strand, within NIAGMN_28710at 47.450 kb on + strand, within NIAGMN_28710at 47.547 kb on - strand, within NIAGMN_28710at 47.572 kb on - strand, within NIAGMN_28710at 47.572 kb on - strand, within NIAGMN_28710at 47.587 kb on + strand, within NIAGMN_28710at 47.588 kb on - strand, within NIAGMN_28710at 47.604 kb on + strand, within NIAGMN_28710at 47.605 kb on - strand, within NIAGMN_28710at 47.605 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.629 kb on - strand, within NIAGMN_28710at 47.673 kb on + strand, within NIAGMN_28710at 47.770 kb on - strandat 47.960 kb on - strand, within NIAGMN_28715at 48.045 kb on + strandat 48.152 kb on + strand, within yncBat 48.153 kb on - strand, within yncBat 48.161 kb on + strand, within yncBat 48.161 kb on + strand, within yncBat 48.162 kb on - strand, within yncBat 48.163 kb on + strand, within yncBat 48.164 kb on - strand, within yncBat 48.164 kb on - strand, within yncBat 48.190 kb on - strand, within yncBat 48.237 kb on + strand, within yncBat 48.238 kb on - strand, within yncBat 48.246 kb on - strand, within yncBat 48.326 kb on + strand, within yncBat 48.355 kb on - strand, within yncBat 48.406 kb on - strand, within yncBat 48.418 kb on + strand, within yncBat 48.660 kb on - strandat 48.678 kb on - strandat 48.701 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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46,578 - +3.7
46,590 + -1.2
46,591 - +3.2
46,591 - +1.6
46,591 - +1.3
46,627 - tap NIAGMN_28695 0.29 +0.3
46,680 - -1.8
46,682 - -2.4
46,685 + -0.9
46,716 + +2.9
46,723 + +0.9
46,723 + -0.7
46,799 - -3.0
46,832 - +1.9
46,875 - +0.8
46,905 - -1.7
46,945 + repA2 NIAGMN_28705 0.12 +0.8
47,014 - repA2 NIAGMN_28705 0.39 -0.1
47,014 - repA2 NIAGMN_28705 0.39 -1.7
47,015 + repA2 NIAGMN_28705 0.39 -1.8
47,015 + repA2 NIAGMN_28705 0.39 +2.0
47,015 + repA2 NIAGMN_28705 0.39 -0.6
47,015 + repA2 NIAGMN_28705 0.39 -0.6
47,016 - repA2 NIAGMN_28705 0.40 -0.0
47,016 - repA2 NIAGMN_28705 0.40 -0.1
47,016 - repA2 NIAGMN_28705 0.40 -0.9
47,016 - repA2 NIAGMN_28705 0.40 -1.7
47,016 - repA2 NIAGMN_28705 0.40 -0.3
47,016 - repA2 NIAGMN_28705 0.40 -2.5
47,038 + repA2 NIAGMN_28705 0.48 -0.3
47,039 - repA2 NIAGMN_28705 0.49 +0.9
47,039 - repA2 NIAGMN_28705 0.49 +1.0
47,039 - repA2 NIAGMN_28705 0.49 -2.3
47,039 - repA2 NIAGMN_28705 0.49 -3.0
47,039 - repA2 NIAGMN_28705 0.49 -4.2
47,039 - repA2 NIAGMN_28705 0.49 -3.3
47,039 - repA2 NIAGMN_28705 0.49 -2.9
47,062 - repA2 NIAGMN_28705 0.58 +0.6
47,062 - repA2 NIAGMN_28705 0.58 -0.4
47,117 + repA2 NIAGMN_28705 0.79 -3.2
47,118 - repA2 NIAGMN_28705 0.80 -1.2
47,118 - repA2 NIAGMN_28705 0.80 -0.2
47,118 - repA2 NIAGMN_28705 0.80 +0.9
47,183 + -1.7
47,187 + -0.8
47,187 + -0.7
47,187 + -2.0
47,188 - +2.3
47,188 - +2.0
47,188 - +3.8
47,213 - +2.3
47,213 - -1.6
47,213 - -2.6
47,215 + +0.8
47,216 - -0.8
47,287 - -1.1
47,287 - -0.3
47,297 - -0.8
47,306 + +3.1
47,318 + +1.1
47,318 + +2.4
47,318 + -2.9
47,318 + -1.3
47,318 + -1.6
47,318 + +1.5
47,318 + +1.6
47,319 - +1.3
47,319 - -0.1
47,321 + +0.3
47,322 - -1.0
47,334 + +2.1
47,335 - -1.3
47,414 + +0.4
47,415 - +0.1
47,415 - +0.5
47,415 - +0.4
47,415 - -1.9
47,450 + NIAGMN_28710 0.12 +2.4
47,450 + NIAGMN_28710 0.12 -2.4
47,547 - NIAGMN_28710 0.41 -2.3
47,572 - NIAGMN_28710 0.48 +0.5
47,572 - NIAGMN_28710 0.48 -1.0
47,587 + NIAGMN_28710 0.53 +2.5
47,588 - NIAGMN_28710 0.53 -1.0
47,604 + NIAGMN_28710 0.58 -1.7
47,605 - NIAGMN_28710 0.58 -1.1
47,605 - NIAGMN_28710 0.58 +0.6
47,629 - NIAGMN_28710 0.65 +0.1
47,629 - NIAGMN_28710 0.65 +0.3
47,629 - NIAGMN_28710 0.65 -1.1
47,673 + NIAGMN_28710 0.78 -0.4
47,770 - +1.0
47,960 - NIAGMN_28715 0.83 +0.5
48,045 + +0.1
48,152 + yncB NIAGMN_28720 0.24 +1.3
48,153 - yncB NIAGMN_28720 0.24 -3.2
48,161 + yncB NIAGMN_28720 0.26 -4.0
48,161 + yncB NIAGMN_28720 0.26 -2.4
48,162 - yncB NIAGMN_28720 0.26 +2.7
48,163 + yncB NIAGMN_28720 0.27 +2.7
48,164 - yncB NIAGMN_28720 0.27 +1.0
48,164 - yncB NIAGMN_28720 0.27 +0.6
48,190 - yncB NIAGMN_28720 0.32 -2.3
48,237 + yncB NIAGMN_28720 0.43 -2.1
48,238 - yncB NIAGMN_28720 0.43 -0.8
48,246 - yncB NIAGMN_28720 0.45 -0.4
48,326 + yncB NIAGMN_28720 0.62 -1.9
48,355 - yncB NIAGMN_28720 0.68 -0.1
48,406 - yncB NIAGMN_28720 0.79 -2.3
48,418 + yncB NIAGMN_28720 0.82 +2.1
48,660 - -0.6
48,678 - +0.3
48,701 - -1.4

Or see this region's nucleotide sequence