Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27665

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrssA and ychJ are separated by 73 nucleotidesychJ and purU are separated by 49 nucleotidespurU and Tyr_trna are separated by 159 nucleotidesTyr_trna and Tyr_trna are separated by 209 nucleotides NIAGMN_27655: rssA - patatin-like phospholipase RssA, at 5,358,595 to 5,359,539 rssA NIAGMN_27660: ychJ - YchJ family protein, at 5,359,613 to 5,360,071 ychJ NIAGMN_27665: purU - formyltetrahydrofolate deformylase, at 5,360,121 to 5,360,963 purU NIAGMN_27670: Tyr_trna - tRNA-Tyr(gta), at 5,361,123 to 5,361,207 _trna NIAGMN_27675: Tyr_trna - tRNA-Tyr(gta), at 5,361,417 to 5,361,501 _trna Position (kb) 5360 5361Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5359.129 kb on - strand, within rssAat 5359.432 kb on - strand, within rssAat 5359.609 kb on - strandat 5359.656 kb on + strandat 5359.657 kb on - strandat 5359.657 kb on - strandat 5359.670 kb on + strand, within ychJat 5359.982 kb on + strand, within ychJat 5359.982 kb on + strand, within ychJat 5360.186 kb on + strandat 5360.439 kb on + strand, within purUat 5360.439 kb on + strand, within purUat 5360.486 kb on + strand, within purUat 5360.491 kb on + strand, within purUat 5360.518 kb on + strand, within purUat 5360.549 kb on - strand, within purUat 5360.625 kb on + strand, within purUat 5360.757 kb on - strand, within purUat 5360.775 kb on + strand, within purUat 5360.893 kb on + strandat 5361.363 kb on + strandat 5361.585 kb on - strandat 5361.930 kb on - strandat 5361.930 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,359,129 - rssA NIAGMN_27655 0.57 +0.6
5,359,432 - rssA NIAGMN_27655 0.89 +0.7
5,359,609 - +2.4
5,359,656 + -0.2
5,359,657 - -0.1
5,359,657 - +1.5
5,359,670 + ychJ NIAGMN_27660 0.12 -0.8
5,359,982 + ychJ NIAGMN_27660 0.80 +2.9
5,359,982 + ychJ NIAGMN_27660 0.80 -1.4
5,360,186 + -3.5
5,360,439 + purU NIAGMN_27665 0.38 -0.2
5,360,439 + purU NIAGMN_27665 0.38 +0.7
5,360,486 + purU NIAGMN_27665 0.43 +1.2
5,360,491 + purU NIAGMN_27665 0.44 +0.1
5,360,518 + purU NIAGMN_27665 0.47 +0.5
5,360,549 - purU NIAGMN_27665 0.51 -0.7
5,360,625 + purU NIAGMN_27665 0.60 -1.1
5,360,757 - purU NIAGMN_27665 0.75 +0.9
5,360,775 + purU NIAGMN_27665 0.78 -3.8
5,360,893 + -3.1
5,361,363 + -1.0
5,361,585 - +0.9
5,361,930 - -0.4
5,361,930 - +0.7

Or see this region's nucleotide sequence