Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eCs1768 and NIAGMN_27485 overlap by 29 nucleotides NIAGMN_27485 and NIAGMN_27490 overlap by 17 nucleotides NIAGMN_27490 and hipB are separated by 76 nucleotides hipB and NIAGMN_27500 are separated by 263 nucleotides NIAGMN_27500 and NIAGMN_27505 are separated by 72 nucleotides NIAGMN_27505 and NIAGMN_27510 are separated by 22 nucleotides
NIAGMN_27480: eCs1768 - DNA-binding protein, at 5,327,653 to 5,328,693
eCs1768
NIAGMN_27485: NIAGMN_27485 - Putative bacterial toxin ydaT, at 5,328,665 to 5,329,216
_27485
NIAGMN_27490: NIAGMN_27490 - transcriptional regulator, at 5,329,200 to 5,329,427
_27490
NIAGMN_27495: hipB - transcriptional regulator, at 5,329,504 to 5,329,911
hipB
NIAGMN_27500: NIAGMN_27500 - hypothetical protein, at 5,330,175 to 5,330,474
_27500
NIAGMN_27505: NIAGMN_27505 - Uncharacterized protein YdfC, at 5,330,547 to 5,330,765
_27505
NIAGMN_27510: NIAGMN_27510 - hypothetical protein, at 5,330,788 to 5,331,195
_27510
Position (kb)
5329
5330 Strain fitness (log2 ratio)
-2
-1
0
1 at 5328.676 kb on + strand at 5328.732 kb on + strand, within NIAGMN_27485 at 5328.732 kb on + strand, within NIAGMN_27485 at 5328.732 kb on + strand, within NIAGMN_27485 at 5328.879 kb on + strand, within NIAGMN_27485 at 5328.879 kb on + strand, within NIAGMN_27485 at 5328.880 kb on - strand, within NIAGMN_27485 at 5328.983 kb on - strand, within NIAGMN_27485
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,328,676 + -1.0 5,328,732 + NIAGMN_27485 0.12 +1.7 5,328,732 + NIAGMN_27485 0.12 -0.6 5,328,732 + NIAGMN_27485 0.12 -2.3 5,328,879 + NIAGMN_27485 0.39 -0.2 5,328,879 + NIAGMN_27485 0.39 -1.1 5,328,880 - NIAGMN_27485 0.39 -0.3 5,328,983 - NIAGMN_27485 0.58 -2.3
Or see this region's nucleotide sequence