Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_26540 and ycjN are separated by 48 nucleotides ycjN and ycjM are separated by 13 nucleotides ycjM and NIAGMN_26555 overlap by 1 nucleotides NIAGMN_26555 and pspE are separated by 209 nucleotides pspE and pspD are separated by 74 nucleotides
NIAGMN_26540: NIAGMN_26540 - ABC transporter substrate-binding protein, at 5,165,495 to 5,166,109
_26540
NIAGMN_26545: ycjN - Putative ABC transporter periplasmic-binding protein YcjN, at 5,166,158 to 5,166,787
ycjN
NIAGMN_26550: ycjM - Glucosylglycerate phosphorylase, at 5,166,801 to 5,168,105
ycjM
NIAGMN_26555: NIAGMN_26555 - Alpha-amylase, at 5,168,105 to 5,168,479
_26555
NIAGMN_26560: pspE - thiosulfate sulfurtransferase PspE, at 5,168,689 to 5,169,003
pspE
NIAGMN_26565: pspD - phage shock protein PspD, at 5,169,078 to 5,169,299
pspD
Position (kb) 5166
5167
5168
5169 Strain fitness (log2 ratio) -1
0
1 at 5165.984 kb on - strand, within NIAGMN_26540 at 5166.052 kb on + strand at 5166.160 kb on - strand at 5166.571 kb on + strand, within ycjN at 5168.877 kb on + strand, within pspE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,165,984 - NIAGMN_26540 0.80 +1.0 5,166,052 + -1.1 5,166,160 - +0.9 5,166,571 + ycjN NIAGMN_26545 0.66 -0.1 5,168,877 + pspE NIAGMN_26560 0.60 +1.4
Or see this region's nucleotide sequence