Strain Fitness in Escherichia coli ECRC102 around NIAGMN_26460

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntNIAGMN_26450 and wcaG are separated by 20 nucleotideswcaG and NIAGMN_26460 are separated by 290 nucleotidesNIAGMN_26460 and ycjG overlap by 26 nucleotides NIAGMN_26450: NIAGMN_26450 - Hydrolase, at 5,146,526 to 5,147,149 _26450 NIAGMN_26455: wcaG - NAD-dependent dehydratase, at 5,147,170 to 5,147,808 wcaG NIAGMN_26460: NIAGMN_26460 - Murein peptide amidase A, at 5,148,099 to 5,148,827 _26460 NIAGMN_26465: ycjG - L-Ala-D/L-Glu epimerase, at 5,148,802 to 5,149,767 ycjG Position (kb) 5148 5149Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5147.840 kb on - strandat 5148.020 kb on + strandat 5148.157 kb on - strandat 5148.472 kb on + strand, within NIAGMN_26460at 5148.611 kb on - strand, within NIAGMN_26460at 5149.299 kb on + strand, within ycjGat 5149.300 kb on - strand, within ycjGat 5149.411 kb on - strand, within ycjGat 5149.729 kb on + strandat 5149.729 kb on + strandat 5149.826 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,147,840 - -3.1
5,148,020 + +2.4
5,148,157 - +0.7
5,148,472 + NIAGMN_26460 0.51 -2.3
5,148,611 - NIAGMN_26460 0.70 -0.6
5,149,299 + ycjG NIAGMN_26465 0.51 +1.3
5,149,300 - ycjG NIAGMN_26465 0.52 +2.2
5,149,411 - ycjG NIAGMN_26465 0.63 +2.0
5,149,729 + +0.9
5,149,729 + +0.7
5,149,826 + +0.7

Or see this region's nucleotide sequence