Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcI and trg are separated by 37 nucleotides trg and NIAGMN_25805 are separated by 340 nucleotides NIAGMN_25805 and yncP are separated by 17 nucleotides yncP and ydcA are separated by 25 nucleotides
NIAGMN_25790: ydcI - Uncharacterized HTH-type transcriptional regulator YdcI, at 5,033,321 to 5,034,244
ydcI
NIAGMN_25795: trg - methyl-accepting chemotaxis protein Trg, at 5,034,282 to 5,035,922
trg
NIAGMN_25805: NIAGMN_25805 - Regulatory protein mokB, at 5,036,263 to 5,036,430
_25805
NIAGMN_25810: yncP - Protein YncP, at 5,036,448 to 5,036,516
yncP
NIAGMN_25815: ydcA - Uncharacterized protein YdcA, at 5,036,542 to 5,036,715
ydcA
Position (kb) 5034
5035
5036 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5033.371 kb on + strand at 5033.458 kb on - strand, within ydcI at 5033.521 kb on + strand, within ydcI at 5033.521 kb on + strand, within ydcI at 5033.521 kb on + strand, within ydcI at 5033.617 kb on + strand, within ydcI at 5033.647 kb on + strand, within ydcI at 5033.896 kb on - strand, within ydcI at 5033.974 kb on - strand, within ydcI at 5034.358 kb on - strand at 5034.376 kb on - strand at 5035.484 kb on - strand, within trg at 5035.512 kb on - strand, within trg at 5035.946 kb on + strand at 5035.983 kb on + strand at 5035.993 kb on + strand at 5036.027 kb on + strand at 5036.163 kb on + strand at 5036.406 kb on - strand, within NIAGMN_25805 at 5036.468 kb on + strand, within yncP at 5036.572 kb on - strand, within ydcA at 5036.572 kb on - strand, within ydcA at 5036.574 kb on + strand, within ydcA at 5036.574 kb on + strand, within ydcA at 5036.575 kb on - strand, within ydcA at 5036.575 kb on - strand, within ydcA at 5036.667 kb on - strand, within ydcA at 5036.718 kb on - strand at 5036.741 kb on + strand at 5036.836 kb on - strand at 5036.896 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,033,371 + -1.0 5,033,458 - ydcI NIAGMN_25790 0.15 +1.1 5,033,521 + ydcI NIAGMN_25790 0.22 +0.6 5,033,521 + ydcI NIAGMN_25790 0.22 +0.8 5,033,521 + ydcI NIAGMN_25790 0.22 -2.5 5,033,617 + ydcI NIAGMN_25790 0.32 +0.5 5,033,647 + ydcI NIAGMN_25790 0.35 -2.0 5,033,896 - ydcI NIAGMN_25790 0.62 +1.9 5,033,974 - ydcI NIAGMN_25790 0.71 +1.7 5,034,358 - +0.0 5,034,376 - -2.7 5,035,484 - trg NIAGMN_25795 0.73 -0.6 5,035,512 - trg NIAGMN_25795 0.75 -0.7 5,035,946 + +0.5 5,035,983 + -0.0 5,035,993 + +0.5 5,036,027 + +0.0 5,036,163 + -2.2 5,036,406 - NIAGMN_25805 0.85 -2.4 5,036,468 + yncP NIAGMN_25810 0.29 +1.8 5,036,572 - ydcA NIAGMN_25815 0.17 +3.2 5,036,572 - ydcA NIAGMN_25815 0.17 -1.7 5,036,574 + ydcA NIAGMN_25815 0.18 -1.0 5,036,574 + ydcA NIAGMN_25815 0.18 +0.7 5,036,575 - ydcA NIAGMN_25815 0.19 -1.4 5,036,575 - ydcA NIAGMN_25815 0.19 -2.2 5,036,667 - ydcA NIAGMN_25815 0.72 -0.2 5,036,718 - -1.1 5,036,741 + -0.2 5,036,836 - +3.0 5,036,896 - +0.4
Or see this region's nucleotide sequence