Strain Fitness in Escherichia coli ECRC102 around NIAGMN_25715

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotD and ydcR are separated by 244 nucleotidesydcR and hicB are separated by 78 nucleotideshicB and hicA are separated by 24 nucleotideshicA and yncJ are separated by 281 nucleotides NIAGMN_25710: potD - spermidine/putrescine ABC transporter substrate-binding protein, at 5,017,339 to 5,018,484 potD NIAGMN_25715: ydcR - Uncharacterized HTH-type transcriptional regulator YdcR, at 5,018,729 to 5,020,135 ydcR NIAGMN_25720: hicB - type II toxin-antitoxin system antitoxin HicB, at 5,020,214 to 5,020,651 hicB NIAGMN_25725: hicA - type II toxin-antitoxin system mRNA interferase toxin HicA, at 5,020,676 to 5,020,852 hicA NIAGMN_25730: yncJ - Uncharacterized protein YncJ, at 5,021,134 to 5,021,304 yncJ Position (kb) 5018 5019 5020 5021Strain fitness (log2 ratio) -2 -1 0 1 2at 5018.025 kb on + strand, within potDat 5018.172 kb on + strand, within potDat 5018.353 kb on - strand, within potDat 5018.507 kb on + strandat 5018.554 kb on - strandat 5018.728 kb on - strandat 5018.983 kb on - strand, within ydcRat 5019.395 kb on - strand, within ydcRat 5019.746 kb on + strand, within ydcRat 5019.942 kb on + strand, within ydcRat 5019.970 kb on + strand, within ydcRat 5019.970 kb on + strand, within ydcRat 5019.971 kb on - strand, within ydcRat 5020.416 kb on - strand, within hicBat 5020.438 kb on + strand, within hicBat 5020.875 kb on + strandat 5020.876 kb on - strandat 5020.934 kb on - strandat 5021.016 kb on - strandat 5021.043 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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5,018,025 + potD NIAGMN_25710 0.60 +1.7
5,018,172 + potD NIAGMN_25710 0.73 -0.3
5,018,353 - potD NIAGMN_25710 0.88 +0.2
5,018,507 + -1.9
5,018,554 - -1.4
5,018,728 - -0.2
5,018,983 - ydcR NIAGMN_25715 0.18 +1.8
5,019,395 - ydcR NIAGMN_25715 0.47 -2.5
5,019,746 + ydcR NIAGMN_25715 0.72 +1.4
5,019,942 + ydcR NIAGMN_25715 0.86 +0.2
5,019,970 + ydcR NIAGMN_25715 0.88 -1.6
5,019,970 + ydcR NIAGMN_25715 0.88 +0.4
5,019,971 - ydcR NIAGMN_25715 0.88 -0.9
5,020,416 - hicB NIAGMN_25720 0.46 -2.5
5,020,438 + hicB NIAGMN_25720 0.51 -1.9
5,020,875 + -1.1
5,020,876 - +0.3
5,020,934 - +2.5
5,021,016 - -2.3
5,021,043 + +1.4

Or see this region's nucleotide sequence