Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_25675 and ydcY are separated by 0 nucleotides ydcY and ortT are separated by 85 nucleotides ortT and patD are separated by 386 nucleotides patD and ydcV are separated by 21 nucleotides ydcV and potB overlap by 11 nucleotides
NIAGMN_25675: NIAGMN_25675 - Putative inner membrane exporter, YdcZ, at 5,011,807 to 5,012,256
_25675
NIAGMN_25680: ydcY - Uncharacterized protein YdcY, at 5,012,257 to 5,012,490
ydcY
NIAGMN_25685: ortT - orphan toxin OrtT, at 5,012,576 to 5,012,749
ortT
NIAGMN_25690: patD - aminobutyraldehyde dehydrogenase, at 5,013,136 to 5,014,560
patD
NIAGMN_25695: ydcV - Inner membrane ABC transporter permease protein YdcV, at 5,014,582 to 5,015,376
ydcV
NIAGMN_25700: potB - spermidine/putrescine ABC transporter permease, at 5,015,366 to 5,016,307
potB
Position (kb)
5013
5014
5015 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5012.255 kb on - strand at 5012.490 kb on + strand at 5012.540 kb on - strand at 5012.751 kb on + strand at 5012.751 kb on + strand at 5012.911 kb on + strand at 5013.009 kb on + strand at 5013.065 kb on - strand at 5013.128 kb on + strand at 5013.131 kb on + strand at 5013.274 kb on - strand at 5013.374 kb on - strand, within patD at 5013.403 kb on - strand, within patD at 5013.485 kb on + strand, within patD at 5013.754 kb on - strand, within patD at 5013.835 kb on - strand, within patD at 5013.835 kb on - strand, within patD at 5013.840 kb on + strand, within patD at 5013.990 kb on + strand, within patD at 5013.991 kb on - strand, within patD at 5014.170 kb on + strand, within patD at 5014.566 kb on - strand at 5014.835 kb on - strand, within ydcV at 5015.132 kb on - strand, within ydcV at 5015.410 kb on - strand at 5015.508 kb on + strand, within potB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 5,012,255 - -0.6 5,012,490 + +1.6 5,012,540 - +0.8 5,012,751 + -1.4 5,012,751 + +1.9 5,012,911 + +0.4 5,013,009 + -1.5 5,013,065 - -0.6 5,013,128 + -0.7 5,013,131 + -0.7 5,013,274 - +0.5 5,013,374 - patD NIAGMN_25690 0.17 +0.9 5,013,403 - patD NIAGMN_25690 0.19 +1.3 5,013,485 + patD NIAGMN_25690 0.24 +0.5 5,013,754 - patD NIAGMN_25690 0.43 +2.4 5,013,835 - patD NIAGMN_25690 0.49 -0.2 5,013,835 - patD NIAGMN_25690 0.49 -3.2 5,013,840 + patD NIAGMN_25690 0.49 +1.5 5,013,990 + patD NIAGMN_25690 0.60 +1.4 5,013,991 - patD NIAGMN_25690 0.60 +2.9 5,014,170 + patD NIAGMN_25690 0.73 -2.0 5,014,566 - +0.5 5,014,835 - ydcV NIAGMN_25695 0.32 +0.9 5,015,132 - ydcV NIAGMN_25695 0.69 +2.7 5,015,410 - -1.7 5,015,508 + potB NIAGMN_25700 0.15 +1.3
Or see this region's nucleotide sequence