Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt csgB and csgA are separated by 40 nucleotides csgA and csgC are separated by 58 nucleotides csgC and NIAGMN_24010 are separated by 120 nucleotides NIAGMN_24010 and ymdB are separated by 94 nucleotides
NIAGMN_23995: csgB - curlin minor subunit CsgB, at 4,696,832 to 4,697,287
csgB
NIAGMN_24000: csgA - curlin major subunit CsgA, at 4,697,328 to 4,697,786
csgA
NIAGMN_24005: csgC - curli assembly protein CsgC, at 4,697,845 to 4,698,177
csgC
NIAGMN_24010: NIAGMN_24010 - Uncharacterized protein YmdA, at 4,698,298 to 4,698,609
_24010
NIAGMN_24015: ymdB - O-acetyl-ADP-ribose deacetylase, at 4,698,704 to 4,699,237
ymdB
Position (kb) 4697
4698
4699 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4696.974 kb on + strand, within csgB at 4697.107 kb on + strand, within csgB at 4697.241 kb on + strand, within csgB at 4697.285 kb on + strand at 4697.403 kb on + strand, within csgA at 4697.625 kb on - strand, within csgA at 4697.666 kb on + strand, within csgA at 4697.675 kb on + strand, within csgA at 4697.675 kb on + strand, within csgA at 4697.723 kb on + strand, within csgA at 4697.784 kb on + strand at 4697.787 kb on + strand at 4697.787 kb on + strand at 4697.798 kb on + strand at 4697.856 kb on - strand at 4697.917 kb on + strand, within csgC at 4697.918 kb on - strand, within csgC at 4697.918 kb on - strand, within csgC at 4697.918 kb on - strand, within csgC at 4698.291 kb on - strand at 4698.604 kb on - strand at 4698.608 kb on - strand at 4698.692 kb on + strand at 4698.715 kb on + strand at 4699.040 kb on + strand, within ymdB at 4699.040 kb on + strand, within ymdB at 4699.041 kb on - strand, within ymdB at 4699.041 kb on - strand, within ymdB at 4699.043 kb on - strand, within ymdB at 4699.166 kb on + strand, within ymdB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,696,974 + csgB NIAGMN_23995 0.31 +1.6 4,697,107 + csgB NIAGMN_23995 0.60 +0.3 4,697,241 + csgB NIAGMN_23995 0.90 -2.4 4,697,285 + +0.4 4,697,403 + csgA NIAGMN_24000 0.16 -0.8 4,697,625 - csgA NIAGMN_24000 0.65 -3.1 4,697,666 + csgA NIAGMN_24000 0.74 +1.3 4,697,675 + csgA NIAGMN_24000 0.76 -0.2 4,697,675 + csgA NIAGMN_24000 0.76 -0.7 4,697,723 + csgA NIAGMN_24000 0.86 -0.2 4,697,784 + +0.3 4,697,787 + -0.6 4,697,787 + +0.0 4,697,798 + -0.3 4,697,856 - +0.6 4,697,917 + csgC NIAGMN_24005 0.22 +0.9 4,697,918 - csgC NIAGMN_24005 0.22 -0.8 4,697,918 - csgC NIAGMN_24005 0.22 +0.1 4,697,918 - csgC NIAGMN_24005 0.22 +1.4 4,698,291 - -1.7 4,698,604 - +2.1 4,698,608 - -2.9 4,698,692 + +2.6 4,698,715 + -0.3 4,699,040 + ymdB NIAGMN_24015 0.63 +0.6 4,699,040 + ymdB NIAGMN_24015 0.63 -1.5 4,699,041 - ymdB NIAGMN_24015 0.63 -1.4 4,699,041 - ymdB NIAGMN_24015 0.63 -1.8 4,699,043 - ymdB NIAGMN_24015 0.63 +2.2 4,699,166 + ymdB NIAGMN_24015 0.87 +0.6
Or see this region's nucleotide sequence