Strain Fitness in Escherichia coli ECRC102 around NIAGMN_23445

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntstxA2c and stxB2a are separated by 11 nucleotidesstxB2a and NIAGMN_23460 are separated by 486 nucleotides NIAGMN_23440: stxA2c - Shiga toxin Stx2c subunit A, at 4,588,270 to 4,589,229 stxA2c NIAGMN_23445: stxB2a - Shiga toxin Stx2a subunit B, at 4,589,241 to 4,589,510 stxB2a NIAGMN_23460: NIAGMN_23460 - sialate O-acetylesterase, at 4,589,997 to 4,591,934 _23460 Position (kb) 4589 4590Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 4588.372 kb on + strand, within stxA2cat 4588.395 kb on - strand, within stxA2cat 4588.427 kb on + strand, within stxA2cat 4588.626 kb on + strand, within stxA2cat 4588.626 kb on + strand, within stxA2cat 4588.627 kb on - strand, within stxA2cat 4588.628 kb on + strand, within stxA2cat 4588.629 kb on - strand, within stxA2cat 4588.779 kb on + strand, within stxA2cat 4588.856 kb on + strand, within stxA2cat 4589.154 kb on + strandat 4589.154 kb on + strandat 4589.210 kb on + strandat 4589.215 kb on - strandat 4589.336 kb on + strand, within stxB2aat 4589.498 kb on + strandat 4589.501 kb on + strandat 4589.501 kb on + strandat 4589.531 kb on - strandat 4589.548 kb on - strandat 4589.735 kb on + strandat 4589.752 kb on + strandat 4589.752 kb on + strandat 4589.754 kb on + strandat 4589.754 kb on + strandat 4589.754 kb on + strandat 4589.755 kb on - strandat 4589.755 kb on - strandat 4589.785 kb on - strandat 4589.791 kb on - strandat 4589.791 kb on - strandat 4589.827 kb on + strandat 4589.828 kb on - strandat 4589.837 kb on - strandat 4589.867 kb on + strandat 4589.910 kb on - strandat 4589.975 kb on - strandat 4590.231 kb on + strand, within NIAGMN_23460at 4590.270 kb on + strand, within NIAGMN_23460at 4590.270 kb on + strand, within NIAGMN_23460at 4590.320 kb on + strand, within NIAGMN_23460at 4590.344 kb on + strand, within NIAGMN_23460at 4590.344 kb on + strand, within NIAGMN_23460

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,588,372 + stxA2c NIAGMN_23440 0.11 -0.3
4,588,395 - stxA2c NIAGMN_23440 0.13 +0.5
4,588,427 + stxA2c NIAGMN_23440 0.16 +3.5
4,588,626 + stxA2c NIAGMN_23440 0.37 -0.0
4,588,626 + stxA2c NIAGMN_23440 0.37 +4.4
4,588,627 - stxA2c NIAGMN_23440 0.37 -0.5
4,588,628 + stxA2c NIAGMN_23440 0.37 -2.0
4,588,629 - stxA2c NIAGMN_23440 0.37 -0.3
4,588,779 + stxA2c NIAGMN_23440 0.53 +1.8
4,588,856 + stxA2c NIAGMN_23440 0.61 +0.1
4,589,154 + +0.6
4,589,154 + -1.8
4,589,210 + +2.9
4,589,215 - -1.2
4,589,336 + stxB2a NIAGMN_23445 0.35 +0.5
4,589,498 + +0.6
4,589,501 + +0.4
4,589,501 + -1.7
4,589,531 - -0.7
4,589,548 - -3.8
4,589,735 + -3.4
4,589,752 + +1.2
4,589,752 + +0.8
4,589,754 + -1.3
4,589,754 + -0.1
4,589,754 + +0.6
4,589,755 - -1.5
4,589,755 - +1.5
4,589,785 - -3.6
4,589,791 - +0.4
4,589,791 - +0.8
4,589,827 + -1.8
4,589,828 - +0.3
4,589,837 - +1.8
4,589,867 + -3.5
4,589,910 - -1.5
4,589,975 - -0.5
4,590,231 + NIAGMN_23460 0.12 +1.1
4,590,270 + NIAGMN_23460 0.14 -1.6
4,590,270 + NIAGMN_23460 0.14 -0.0
4,590,320 + NIAGMN_23460 0.17 +2.0
4,590,344 + NIAGMN_23460 0.18 +2.1
4,590,344 + NIAGMN_23460 0.18 -4.4

Or see this region's nucleotide sequence