Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lexA and NIAGMN_23325 are separated by 117 nucleotides NIAGMN_23325 and NIAGMN_23330 are separated by 141 nucleotides NIAGMN_23330 and NIAGMN_23335 are separated by 32 nucleotides NIAGMN_23335 and NIAGMN_23340 overlap by 8 nucleotides NIAGMN_23340 and dnaB overlap by 11 nucleotides
NIAGMN_23320: lexA - phage repressor, at 4,578,217 to 4,578,870
lexA
NIAGMN_23325: NIAGMN_23325 - XRE family transcriptional regulator, at 4,578,988 to 4,579,203
_23325
NIAGMN_23330: NIAGMN_23330 - Bacteriophage CII protein, at 4,579,345 to 4,579,641
_23330
NIAGMN_23335: NIAGMN_23335 - Uncharacterized protein, at 4,579,674 to 4,579,820
_23335
NIAGMN_23340: NIAGMN_23340 - DNA replication protein, at 4,579,813 to 4,580,712
_23340
NIAGMN_23345: dnaB - helicase DnaB, at 4,580,702 to 4,582,138
dnaB
Position (kb) 4579
4580 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4579.024 kb on - strand, within NIAGMN_23325 at 4579.618 kb on - strand at 4579.720 kb on + strand, within NIAGMN_23335 at 4579.721 kb on - strand, within NIAGMN_23335 at 4579.828 kb on - strand at 4580.001 kb on - strand, within NIAGMN_23340 at 4580.101 kb on + strand, within NIAGMN_23340 at 4580.183 kb on - strand, within NIAGMN_23340 at 4580.269 kb on - strand, within NIAGMN_23340 at 4580.314 kb on - strand, within NIAGMN_23340 at 4580.340 kb on - strand, within NIAGMN_23340 at 4580.569 kb on + strand, within NIAGMN_23340 at 4580.570 kb on - strand, within NIAGMN_23340 at 4580.570 kb on - strand, within NIAGMN_23340 at 4580.574 kb on - strand, within NIAGMN_23340 at 4580.670 kb on + strand at 4580.671 kb on - strand at 4580.671 kb on - strand at 4580.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,579,024 - NIAGMN_23325 0.17 +1.7 4,579,618 - +1.7 4,579,720 + NIAGMN_23335 0.31 +3.2 4,579,721 - NIAGMN_23335 0.32 +0.8 4,579,828 - +0.7 4,580,001 - NIAGMN_23340 0.21 -0.0 4,580,101 + NIAGMN_23340 0.32 -2.5 4,580,183 - NIAGMN_23340 0.41 -0.1 4,580,269 - NIAGMN_23340 0.51 -0.3 4,580,314 - NIAGMN_23340 0.56 -2.5 4,580,340 - NIAGMN_23340 0.59 +1.0 4,580,569 + NIAGMN_23340 0.84 -1.9 4,580,570 - NIAGMN_23340 0.84 +0.1 4,580,570 - NIAGMN_23340 0.84 +0.1 4,580,574 - NIAGMN_23340 0.85 -0.5 4,580,670 + -0.4 4,580,671 - -1.0 4,580,671 - -0.3 4,580,674 + +2.2
Or see this region's nucleotide sequence