Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_23060 and NIAGMN_23065 overlap by 4 nucleotides NIAGMN_23065 and appC are separated by 133 nucleotides appC and appB are separated by 11 nucleotides
NIAGMN_23060: NIAGMN_23060 - hydrogenase 1 maturation protease, at 4,536,779 to 4,537,177
_23060
NIAGMN_23065: NIAGMN_23065 - hydrogenase expression/formation protein, at 4,537,174 to 4,538,031
_23065
NIAGMN_23070: appC - cytochrome bd-II oxidase subunit 1, at 4,538,165 to 4,539,709
appC
NIAGMN_23075: appB - cytochrome d ubiquinol oxidase subunit II, at 4,539,721 to 4,540,857
appB
Position (kb)
4538
4539
4540 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4537.840 kb on + strand, within NIAGMN_23065 at 4537.841 kb on - strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.877 kb on + strand, within NIAGMN_23065 at 4537.895 kb on + strand, within NIAGMN_23065 at 4537.997 kb on + strand at 4537.997 kb on + strand at 4538.029 kb on + strand at 4538.059 kb on + strand at 4538.401 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.423 kb on + strand, within appC at 4538.535 kb on - strand, within appC at 4538.758 kb on + strand, within appC at 4538.759 kb on - strand, within appC at 4539.063 kb on + strand, within appC at 4539.130 kb on + strand, within appC at 4539.278 kb on + strand, within appC at 4539.650 kb on + strand at 4539.650 kb on + strand at 4539.650 kb on + strand at 4539.870 kb on + strand, within appB at 4539.870 kb on + strand, within appB at 4539.932 kb on + strand, within appB at 4539.955 kb on + strand, within appB at 4539.979 kb on + strand, within appB at 4539.979 kb on + strand, within appB at 4539.979 kb on + strand, within appB at 4540.229 kb on + strand, within appB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,537,840 + NIAGMN_23065 0.78 -1.1 4,537,841 - NIAGMN_23065 0.78 +0.6 4,537,877 + NIAGMN_23065 0.82 +0.6 4,537,877 + NIAGMN_23065 0.82 -0.7 4,537,877 + NIAGMN_23065 0.82 -0.9 4,537,877 + NIAGMN_23065 0.82 +1.4 4,537,895 + NIAGMN_23065 0.84 -1.2 4,537,997 + +1.2 4,537,997 + +0.5 4,538,029 + +0.1 4,538,059 + -1.0 4,538,401 + appC NIAGMN_23070 0.15 -1.0 4,538,423 + appC NIAGMN_23070 0.17 -0.0 4,538,423 + appC NIAGMN_23070 0.17 +0.4 4,538,423 + appC NIAGMN_23070 0.17 -1.2 4,538,423 + appC NIAGMN_23070 0.17 +1.0 4,538,535 - appC NIAGMN_23070 0.24 +0.3 4,538,758 + appC NIAGMN_23070 0.38 -0.1 4,538,759 - appC NIAGMN_23070 0.38 -2.9 4,539,063 + appC NIAGMN_23070 0.58 +2.1 4,539,130 + appC NIAGMN_23070 0.62 +0.4 4,539,278 + appC NIAGMN_23070 0.72 -1.1 4,539,650 + +0.6 4,539,650 + -2.7 4,539,650 + +0.1 4,539,870 + appB NIAGMN_23075 0.13 +0.6 4,539,870 + appB NIAGMN_23075 0.13 -0.2 4,539,932 + appB NIAGMN_23075 0.19 -0.3 4,539,955 + appB NIAGMN_23075 0.21 +1.3 4,539,979 + appB NIAGMN_23075 0.23 -5.7 4,539,979 + appB NIAGMN_23075 0.23 -1.5 4,539,979 + appB NIAGMN_23075 0.23 -1.9 4,540,229 + appB NIAGMN_23075 0.45 -1.9
Or see this region's nucleotide sequence