Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobU and cobS overlap by 4 nucleotides cobS and cobT are separated by 11 nucleotides cobT and ldtA are separated by 61 nucleotides
NIAGMN_22630: cobU - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 4,465,141 to 4,465,683
cobU
NIAGMN_22635: cobS - adenosylcobinamide-GDP ribazoletransferase, at 4,465,680 to 4,466,423
cobS
NIAGMN_22640: cobT - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,466,435 to 4,467,514
cobT
NIAGMN_22645: ldtA - L,D-transpeptidase, at 4,467,576 to 4,468,511
ldtA
Position (kb) 4466
4467
4468 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4465.529 kb on + strand, within cobU at 4465.529 kb on + strand, within cobU at 4465.529 kb on + strand, within cobU at 4465.529 kb on + strand, within cobU at 4465.529 kb on + strand, within cobU at 4465.530 kb on - strand, within cobU at 4465.643 kb on + strand at 4465.643 kb on + strand at 4465.713 kb on - strand at 4465.804 kb on + strand, within cobS at 4466.005 kb on + strand, within cobS at 4466.005 kb on + strand, within cobS at 4466.151 kb on + strand, within cobS at 4466.151 kb on + strand, within cobS at 4466.151 kb on + strand, within cobS at 4466.163 kb on + strand, within cobS at 4466.291 kb on + strand, within cobS at 4466.291 kb on + strand, within cobS at 4466.462 kb on - strand at 4466.488 kb on + strand at 4466.488 kb on + strand at 4466.668 kb on + strand, within cobT at 4466.669 kb on - strand, within cobT at 4466.770 kb on - strand, within cobT at 4466.782 kb on + strand, within cobT at 4466.836 kb on + strand, within cobT at 4466.904 kb on + strand, within cobT at 4466.904 kb on + strand, within cobT at 4466.912 kb on - strand, within cobT at 4466.952 kb on + strand, within cobT at 4467.074 kb on - strand, within cobT at 4467.239 kb on + strand, within cobT at 4467.329 kb on - strand, within cobT at 4467.330 kb on + strand, within cobT at 4467.440 kb on + strand at 4467.630 kb on - strand at 4467.764 kb on + strand, within ldtA at 4467.923 kb on + strand, within ldtA at 4467.942 kb on - strand, within ldtA at 4468.138 kb on + strand, within ldtA at 4468.139 kb on - strand, within ldtA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,465,529 + cobU NIAGMN_22630 0.71 +2.2 4,465,529 + cobU NIAGMN_22630 0.71 -0.4 4,465,529 + cobU NIAGMN_22630 0.71 -1.7 4,465,529 + cobU NIAGMN_22630 0.71 -0.1 4,465,529 + cobU NIAGMN_22630 0.71 -0.8 4,465,530 - cobU NIAGMN_22630 0.72 +1.6 4,465,643 + +1.6 4,465,643 + +0.6 4,465,713 - +0.1 4,465,804 + cobS NIAGMN_22635 0.17 -0.8 4,466,005 + cobS NIAGMN_22635 0.44 +0.4 4,466,005 + cobS NIAGMN_22635 0.44 +2.4 4,466,151 + cobS NIAGMN_22635 0.63 -1.4 4,466,151 + cobS NIAGMN_22635 0.63 +2.7 4,466,151 + cobS NIAGMN_22635 0.63 -1.2 4,466,163 + cobS NIAGMN_22635 0.65 +0.7 4,466,291 + cobS NIAGMN_22635 0.82 -3.0 4,466,291 + cobS NIAGMN_22635 0.82 -0.5 4,466,462 - -0.1 4,466,488 + +0.6 4,466,488 + +0.0 4,466,668 + cobT NIAGMN_22640 0.22 -1.0 4,466,669 - cobT NIAGMN_22640 0.22 +0.6 4,466,770 - cobT NIAGMN_22640 0.31 -1.1 4,466,782 + cobT NIAGMN_22640 0.32 +1.8 4,466,836 + cobT NIAGMN_22640 0.37 -1.9 4,466,904 + cobT NIAGMN_22640 0.43 +1.1 4,466,904 + cobT NIAGMN_22640 0.43 -1.5 4,466,912 - cobT NIAGMN_22640 0.44 +0.2 4,466,952 + cobT NIAGMN_22640 0.48 +0.1 4,467,074 - cobT NIAGMN_22640 0.59 +0.8 4,467,239 + cobT NIAGMN_22640 0.74 -0.5 4,467,329 - cobT NIAGMN_22640 0.83 -2.3 4,467,330 + cobT NIAGMN_22640 0.83 +1.0 4,467,440 + -2.7 4,467,630 - +1.2 4,467,764 + ldtA NIAGMN_22645 0.20 -1.4 4,467,923 + ldtA NIAGMN_22645 0.37 -1.7 4,467,942 - ldtA NIAGMN_22645 0.39 -0.7 4,468,138 + ldtA NIAGMN_22645 0.60 +0.4 4,468,139 - ldtA NIAGMN_22645 0.60 -0.5
Or see this region's nucleotide sequence