Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wzzB and hisIE are separated by 96 nucleotides hisIE and hisF overlap by 7 nucleotides hisF and hisA overlap by 19 nucleotides hisA and hisH overlap by 1 nucleotides
NIAGMN_22425: wzzB - LPS O-antigen chain length determinant protein WzzB, at 4,430,008 to 4,430,985
wzzB
NIAGMN_22430: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 4,431,082 to 4,431,693
hisIE
NIAGMN_22435: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 4,431,687 to 4,432,463
hisF
NIAGMN_22440: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 4,432,445 to 4,433,182
hisA
NIAGMN_22445: hisH - imidazole glycerol phosphate synthase subunit HisH, at 4,433,182 to 4,433,772
hisH
Position (kb) 4431
4432
4433 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4430.770 kb on + strand, within wzzB at 4430.842 kb on + strand, within wzzB at 4430.843 kb on - strand, within wzzB at 4430.846 kb on + strand, within wzzB at 4430.861 kb on + strand, within wzzB at 4430.861 kb on + strand, within wzzB at 4430.865 kb on + strand, within wzzB at 4430.955 kb on + strand at 4430.968 kb on - strand at 4430.971 kb on + strand at 4431.149 kb on - strand, within hisIE at 4431.206 kb on - strand, within hisIE at 4432.045 kb on + strand, within hisF at 4432.080 kb on - strand, within hisF at 4432.092 kb on - strand, within hisF at 4432.515 kb on - strand at 4432.515 kb on - strand at 4432.518 kb on - strand at 4432.624 kb on - strand, within hisA at 4432.626 kb on - strand, within hisA at 4432.626 kb on - strand, within hisA at 4432.705 kb on - strand, within hisA at 4433.177 kb on - strand at 4433.256 kb on - strand, within hisH at 4433.263 kb on - strand, within hisH at 4433.302 kb on - strand, within hisH
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,430,770 + wzzB NIAGMN_22425 0.78 +0.4 4,430,842 + wzzB NIAGMN_22425 0.85 +1.1 4,430,843 - wzzB NIAGMN_22425 0.85 -0.3 4,430,846 + wzzB NIAGMN_22425 0.86 -1.4 4,430,861 + wzzB NIAGMN_22425 0.87 +3.3 4,430,861 + wzzB NIAGMN_22425 0.87 -1.4 4,430,865 + wzzB NIAGMN_22425 0.88 +0.7 4,430,955 + +1.1 4,430,968 - +2.9 4,430,971 + +0.8 4,431,149 - hisIE NIAGMN_22430 0.11 -1.6 4,431,206 - hisIE NIAGMN_22430 0.20 -1.4 4,432,045 + hisF NIAGMN_22435 0.46 +1.2 4,432,080 - hisF NIAGMN_22435 0.51 -0.5 4,432,092 - hisF NIAGMN_22435 0.52 +1.3 4,432,515 - -1.8 4,432,515 - -2.0 4,432,518 - -0.9 4,432,624 - hisA NIAGMN_22440 0.24 -0.8 4,432,626 - hisA NIAGMN_22440 0.25 +0.7 4,432,626 - hisA NIAGMN_22440 0.25 -1.5 4,432,705 - hisA NIAGMN_22440 0.35 -0.6 4,433,177 - -0.5 4,433,256 - hisH NIAGMN_22445 0.13 -0.5 4,433,263 - hisH NIAGMN_22445 0.14 -0.7 4,433,302 - hisH NIAGMN_22445 0.20 -2.2
Or see this region's nucleotide sequence