Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojI and mqo are separated by 217 nucleotides mqo and eco are separated by 149 nucleotides eco and NIAGMN_21000 are separated by 133 nucleotides NIAGMN_21000 and NIAGMN_21005 are separated by 51 nucleotides
NIAGMN_20985: yojI - microcin J25 efflux ABC transporter YojI, at 4,158,756 to 4,160,399
yojI
NIAGMN_20990: mqo - malate dehydrogenase (quinone), at 4,160,617 to 4,162,263
mqo
NIAGMN_20995: eco - serine protease inhibitor ecotin, at 4,162,413 to 4,162,901
eco
NIAGMN_21000: NIAGMN_21000 - hypothetical protein, at 4,163,035 to 4,163,199
_21000
NIAGMN_21005: NIAGMN_21005 - Orf 5, at 4,163,251 to 4,163,292
_21005
Position (kb)
4160
4161
4162
4163 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4159.873 kb on - strand, within yojI at 4160.063 kb on + strand, within yojI at 4160.162 kb on + strand, within yojI at 4160.220 kb on + strand, within yojI at 4160.221 kb on - strand, within yojI at 4160.289 kb on + strand at 4160.289 kb on + strand at 4160.397 kb on + strand at 4160.398 kb on - strand at 4160.400 kb on + strand at 4160.446 kb on + strand at 4160.474 kb on - strand at 4160.611 kb on - strand at 4160.717 kb on + strand at 4160.717 kb on + strand at 4160.761 kb on + strand at 4160.762 kb on - strand at 4160.765 kb on + strand at 4161.019 kb on - strand, within mqo at 4161.019 kb on - strand, within mqo at 4161.261 kb on - strand, within mqo at 4161.285 kb on + strand, within mqo at 4161.554 kb on - strand, within mqo at 4161.717 kb on + strand, within mqo at 4161.826 kb on + strand, within mqo at 4161.826 kb on + strand, within mqo at 4161.826 kb on + strand, within mqo at 4161.917 kb on + strand, within mqo at 4162.034 kb on + strand, within mqo at 4162.096 kb on + strand, within mqo at 4162.097 kb on - strand, within mqo at 4162.147 kb on + strand at 4162.197 kb on + strand at 4162.197 kb on + strand at 4162.198 kb on - strand at 4162.198 kb on - strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.261 kb on + strand at 4162.286 kb on + strand at 4162.303 kb on - strand at 4162.352 kb on - strand at 4162.423 kb on - strand at 4162.473 kb on + strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.474 kb on - strand, within eco at 4162.488 kb on - strand, within eco at 4162.490 kb on - strand, within eco at 4162.490 kb on - strand, within eco at 4162.517 kb on - strand, within eco at 4162.611 kb on - strand, within eco at 4162.628 kb on - strand, within eco at 4162.628 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.631 kb on - strand, within eco at 4162.740 kb on - strand, within eco at 4162.926 kb on - strand at 4162.961 kb on - strand at 4162.961 kb on - strand at 4162.993 kb on - strand at 4163.068 kb on + strand, within NIAGMN_21000 at 4163.094 kb on - strand, within NIAGMN_21000 at 4163.215 kb on + strand at 4163.215 kb on + strand at 4163.218 kb on + strand at 4163.219 kb on - strand at 4163.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,159,873 - yojI NIAGMN_20985 0.68 +0.7 4,160,063 + yojI NIAGMN_20985 0.80 -2.8 4,160,162 + yojI NIAGMN_20985 0.86 +1.1 4,160,220 + yojI NIAGMN_20985 0.89 -2.5 4,160,221 - yojI NIAGMN_20985 0.89 -1.1 4,160,289 + +0.7 4,160,289 + -1.2 4,160,397 + -1.8 4,160,398 - +1.3 4,160,400 + +0.6 4,160,446 + -1.1 4,160,474 - +2.8 4,160,611 - +1.7 4,160,717 + -1.8 4,160,717 + -2.1 4,160,761 + +0.2 4,160,762 - -0.7 4,160,765 + +0.8 4,161,019 - mqo NIAGMN_20990 0.24 -0.6 4,161,019 - mqo NIAGMN_20990 0.24 +0.0 4,161,261 - mqo NIAGMN_20990 0.39 +1.6 4,161,285 + mqo NIAGMN_20990 0.41 +2.2 4,161,554 - mqo NIAGMN_20990 0.57 +0.1 4,161,717 + mqo NIAGMN_20990 0.67 +1.8 4,161,826 + mqo NIAGMN_20990 0.73 -1.1 4,161,826 + mqo NIAGMN_20990 0.73 -2.5 4,161,826 + mqo NIAGMN_20990 0.73 -0.8 4,161,917 + mqo NIAGMN_20990 0.79 -0.9 4,162,034 + mqo NIAGMN_20990 0.86 -0.2 4,162,096 + mqo NIAGMN_20990 0.90 -0.5 4,162,097 - mqo NIAGMN_20990 0.90 +0.4 4,162,147 + +0.7 4,162,197 + -1.4 4,162,197 + +1.0 4,162,198 - +1.0 4,162,198 - -1.8 4,162,261 + -1.7 4,162,261 + -3.0 4,162,261 + -2.3 4,162,261 + +0.2 4,162,261 + -0.0 4,162,261 + -0.8 4,162,261 + +0.2 4,162,286 + +2.5 4,162,303 - +2.8 4,162,352 - +2.5 4,162,423 - -0.2 4,162,473 + eco NIAGMN_20995 0.12 -2.1 4,162,474 - eco NIAGMN_20995 0.12 +1.0 4,162,474 - eco NIAGMN_20995 0.12 -0.1 4,162,474 - eco NIAGMN_20995 0.12 +0.1 4,162,474 - eco NIAGMN_20995 0.12 +0.1 4,162,488 - eco NIAGMN_20995 0.15 +0.1 4,162,490 - eco NIAGMN_20995 0.16 +1.5 4,162,490 - eco NIAGMN_20995 0.16 +2.1 4,162,517 - eco NIAGMN_20995 0.21 -2.2 4,162,611 - eco NIAGMN_20995 0.40 +0.6 4,162,628 - eco NIAGMN_20995 0.44 +0.3 4,162,628 - eco NIAGMN_20995 0.44 +1.8 4,162,631 - eco NIAGMN_20995 0.45 +0.7 4,162,631 - eco NIAGMN_20995 0.45 +2.9 4,162,631 - eco NIAGMN_20995 0.45 -0.1 4,162,740 - eco NIAGMN_20995 0.67 -2.5 4,162,926 - -1.7 4,162,961 - -1.2 4,162,961 - -1.6 4,162,993 - +1.8 4,163,068 + NIAGMN_21000 0.20 -0.3 4,163,094 - NIAGMN_21000 0.36 +0.8 4,163,215 + -0.3 4,163,215 + +1.3 4,163,218 + +3.3 4,163,219 - -2.0 4,163,247 - +0.2
Or see this region's nucleotide sequence