Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gyrA and yfaA are separated by 282 nucleotides yfaA and yfaT overlap by 4 nucleotides yfaT and yfaS are separated by 143 nucleotides
NIAGMN_20900: gyrA - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 4,135,168 to 4,137,795
gyrA
NIAGMN_20905: yfaA - DUF2138 domain-containing protein, at 4,138,078 to 4,139,631
yfaA
NIAGMN_20910: yfaT - Uncharacterized protein YfaT, at 4,139,628 to 4,140,251
yfaT
NIAGMN_20915: yfaS - Uncharacterized conserved protein YfaS, alpha-2-macroglobulin family, at 4,140,395 to 4,144,789
yfaS
Position (kb)
4138
4139
4140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4137.793 kb on + strand at 4137.799 kb on + strand at 4138.213 kb on + strand at 4138.372 kb on + strand, within yfaA at 4138.372 kb on + strand, within yfaA at 4138.373 kb on - strand, within yfaA at 4138.494 kb on + strand, within yfaA at 4138.549 kb on + strand, within yfaA at 4138.829 kb on + strand, within yfaA at 4138.830 kb on - strand, within yfaA at 4138.850 kb on + strand, within yfaA at 4138.850 kb on + strand, within yfaA at 4138.851 kb on - strand, within yfaA at 4138.851 kb on - strand, within yfaA at 4138.887 kb on + strand, within yfaA at 4139.388 kb on - strand, within yfaA at 4139.388 kb on - strand, within yfaA at 4139.388 kb on - strand, within yfaA at 4139.417 kb on - strand, within yfaA at 4139.419 kb on - strand, within yfaA at 4139.516 kb on + strand at 4139.558 kb on - strand at 4139.612 kb on + strand at 4139.877 kb on + strand, within yfaT at 4139.962 kb on - strand, within yfaT at 4139.989 kb on - strand, within yfaT at 4139.989 kb on - strand, within yfaT at 4140.017 kb on - strand, within yfaT at 4140.074 kb on - strand, within yfaT at 4140.074 kb on - strand, within yfaT at 4140.094 kb on - strand, within yfaT at 4140.103 kb on - strand, within yfaT at 4140.164 kb on + strand, within yfaT at 4140.165 kb on - strand, within yfaT at 4140.165 kb on - strand, within yfaT at 4140.323 kb on + strand at 4140.323 kb on + strand at 4140.324 kb on - strand at 4140.471 kb on - strand at 4140.476 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,137,793 + +0.2 4,137,799 + +1.2 4,138,213 + +0.3 4,138,372 + yfaA NIAGMN_20905 0.19 +1.2 4,138,372 + yfaA NIAGMN_20905 0.19 -2.8 4,138,373 - yfaA NIAGMN_20905 0.19 +0.8 4,138,494 + yfaA NIAGMN_20905 0.27 +0.4 4,138,549 + yfaA NIAGMN_20905 0.30 +2.3 4,138,829 + yfaA NIAGMN_20905 0.48 +0.8 4,138,830 - yfaA NIAGMN_20905 0.48 +0.0 4,138,850 + yfaA NIAGMN_20905 0.50 -0.3 4,138,850 + yfaA NIAGMN_20905 0.50 +0.3 4,138,851 - yfaA NIAGMN_20905 0.50 -0.9 4,138,851 - yfaA NIAGMN_20905 0.50 -0.2 4,138,887 + yfaA NIAGMN_20905 0.52 +3.0 4,139,388 - yfaA NIAGMN_20905 0.84 +1.1 4,139,388 - yfaA NIAGMN_20905 0.84 -0.1 4,139,388 - yfaA NIAGMN_20905 0.84 -0.6 4,139,417 - yfaA NIAGMN_20905 0.86 -0.1 4,139,419 - yfaA NIAGMN_20905 0.86 -1.5 4,139,516 + +0.0 4,139,558 - -1.8 4,139,612 + +2.5 4,139,877 + yfaT NIAGMN_20910 0.40 -1.8 4,139,962 - yfaT NIAGMN_20910 0.54 -1.3 4,139,989 - yfaT NIAGMN_20910 0.58 +2.8 4,139,989 - yfaT NIAGMN_20910 0.58 -0.9 4,140,017 - yfaT NIAGMN_20910 0.62 -0.9 4,140,074 - yfaT NIAGMN_20910 0.71 +0.4 4,140,074 - yfaT NIAGMN_20910 0.71 -2.1 4,140,094 - yfaT NIAGMN_20910 0.75 +2.3 4,140,103 - yfaT NIAGMN_20910 0.76 -0.8 4,140,164 + yfaT NIAGMN_20910 0.86 +1.0 4,140,165 - yfaT NIAGMN_20910 0.86 +0.7 4,140,165 - yfaT NIAGMN_20910 0.86 -1.7 4,140,323 + -0.5 4,140,323 + -1.1 4,140,324 - -0.7 4,140,471 - +2.2 4,140,476 - -1.2
Or see this region's nucleotide sequence