Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menD and menH overlap by 4 nucleotides menH and menB are separated by 14 nucleotides menB and menC overlap by 1 nucleotides
NIAGMN_20705: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene- 1-carboxylic-acid synthase, at 4,091,899 to 4,093,569
menD
NIAGMN_20710: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 4,093,566 to 4,094,324
menH
NIAGMN_20715: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,094,339 to 4,095,196
menB
NIAGMN_20720: menC - o-succinylbenzoate synthase, at 4,095,196 to 4,096,158
menC
Position (kb)
4093
4094
4095 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4092.814 kb on + strand, within menD at 4092.815 kb on - strand, within menD at 4092.875 kb on + strand, within menD at 4092.875 kb on + strand, within menD at 4092.885 kb on + strand, within menD at 4092.885 kb on + strand, within menD at 4093.033 kb on + strand, within menD at 4093.033 kb on + strand, within menD at 4093.079 kb on + strand, within menD at 4093.080 kb on - strand, within menD at 4093.290 kb on - strand, within menD at 4093.457 kb on + strand at 4093.477 kb on - strand at 4093.493 kb on + strand at 4093.565 kb on + strand at 4093.565 kb on + strand at 4093.565 kb on + strand at 4093.566 kb on - strand at 4093.566 kb on - strand at 4093.606 kb on + strand at 4093.695 kb on + strand, within menH at 4093.695 kb on + strand, within menH at 4093.695 kb on + strand, within menH at 4093.696 kb on - strand, within menH at 4093.696 kb on - strand, within menH at 4093.696 kb on - strand, within menH at 4093.696 kb on - strand, within menH at 4093.696 kb on - strand, within menH at 4093.766 kb on - strand, within menH at 4093.773 kb on - strand, within menH at 4093.818 kb on + strand, within menH at 4094.137 kb on + strand, within menH at 4094.137 kb on + strand, within menH at 4094.138 kb on - strand, within menH at 4094.138 kb on - strand, within menH at 4094.175 kb on + strand, within menH at 4094.179 kb on + strand, within menH at 4094.179 kb on + strand, within menH at 4094.180 kb on - strand, within menH at 4094.180 kb on - strand, within menH at 4094.261 kb on + strand at 4094.262 kb on - strand at 4094.327 kb on - strand at 4094.338 kb on + strand at 4094.646 kb on + strand, within menB at 4094.647 kb on - strand, within menB at 4094.687 kb on - strand, within menB at 4094.782 kb on + strand, within menB at 4094.846 kb on + strand, within menB at 4095.110 kb on + strand, within menB at 4095.110 kb on + strand, within menB at 4095.110 kb on + strand, within menB at 4095.212 kb on + strand at 4095.212 kb on + strand at 4095.213 kb on - strand at 4095.286 kb on + strand at 4095.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,092,814 + menD NIAGMN_20705 0.55 +3.6 4,092,815 - menD NIAGMN_20705 0.55 +0.5 4,092,875 + menD NIAGMN_20705 0.58 -2.3 4,092,875 + menD NIAGMN_20705 0.58 +0.8 4,092,885 + menD NIAGMN_20705 0.59 +1.8 4,092,885 + menD NIAGMN_20705 0.59 +1.6 4,093,033 + menD NIAGMN_20705 0.68 -2.1 4,093,033 + menD NIAGMN_20705 0.68 +1.1 4,093,079 + menD NIAGMN_20705 0.71 +0.6 4,093,080 - menD NIAGMN_20705 0.71 +0.5 4,093,290 - menD NIAGMN_20705 0.83 -1.0 4,093,457 + -1.6 4,093,477 - -1.1 4,093,493 + -0.4 4,093,565 + -1.3 4,093,565 + +0.2 4,093,565 + +0.1 4,093,566 - -0.7 4,093,566 - -1.0 4,093,606 + +2.8 4,093,695 + menH NIAGMN_20710 0.17 -0.6 4,093,695 + menH NIAGMN_20710 0.17 +0.2 4,093,695 + menH NIAGMN_20710 0.17 +0.9 4,093,696 - menH NIAGMN_20710 0.17 +1.2 4,093,696 - menH NIAGMN_20710 0.17 +0.2 4,093,696 - menH NIAGMN_20710 0.17 +0.8 4,093,696 - menH NIAGMN_20710 0.17 +0.1 4,093,696 - menH NIAGMN_20710 0.17 +2.0 4,093,766 - menH NIAGMN_20710 0.26 -0.8 4,093,773 - menH NIAGMN_20710 0.27 -2.3 4,093,818 + menH NIAGMN_20710 0.33 -0.7 4,094,137 + menH NIAGMN_20710 0.75 +1.6 4,094,137 + menH NIAGMN_20710 0.75 +2.2 4,094,138 - menH NIAGMN_20710 0.75 -3.2 4,094,138 - menH NIAGMN_20710 0.75 -1.6 4,094,175 + menH NIAGMN_20710 0.80 -1.3 4,094,179 + menH NIAGMN_20710 0.81 +1.2 4,094,179 + menH NIAGMN_20710 0.81 +0.2 4,094,180 - menH NIAGMN_20710 0.81 -0.0 4,094,180 - menH NIAGMN_20710 0.81 -2.5 4,094,261 + -3.0 4,094,262 - -0.3 4,094,327 - +2.0 4,094,338 + +1.0 4,094,646 + menB NIAGMN_20715 0.36 -1.0 4,094,647 - menB NIAGMN_20715 0.36 -1.9 4,094,687 - menB NIAGMN_20715 0.41 +0.8 4,094,782 + menB NIAGMN_20715 0.52 -1.8 4,094,846 + menB NIAGMN_20715 0.59 -1.9 4,095,110 + menB NIAGMN_20715 0.90 -1.9 4,095,110 + menB NIAGMN_20715 0.90 -1.7 4,095,110 + menB NIAGMN_20715 0.90 -1.7 4,095,212 + -1.0 4,095,212 + -2.1 4,095,213 - -1.2 4,095,286 + -0.4 4,095,287 - +0.6
Or see this region's nucleotide sequence