Strain Fitness in Escherichia coli ECRC102 around NIAGMN_20185

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoxc and yfdV are separated by 69 nucleotidesyfdV and NIAGMN_20190 are separated by 73 nucleotidesNIAGMN_20190 and yfdE are separated by 32 nucleotides NIAGMN_20180: oxc - oxalyl-CoA decarboxylase, at 3,981,589 to 3,983,283 oxc NIAGMN_20185: yfdV - transporter YfdV, at 3,983,353 to 3,984,297 yfdV NIAGMN_20190: NIAGMN_20190 - CoA-transferase, at 3,984,371 to 3,984,601 _20190 NIAGMN_20195: yfdE - CoA:oxalate CoA-transferase, at 3,984,634 to 3,985,512 yfdE Position (kb) 3983 3984 3985Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3982.813 kb on + strand, within oxcat 3982.813 kb on + strand, within oxcat 3982.930 kb on - strand, within oxcat 3982.975 kb on + strand, within oxcat 3983.102 kb on + strand, within oxcat 3983.200 kb on - strandat 3983.658 kb on - strand, within yfdVat 3983.838 kb on + strand, within yfdVat 3983.879 kb on + strand, within yfdVat 3984.202 kb on + strand, within yfdVat 3984.239 kb on + strandat 3984.274 kb on + strandat 3984.436 kb on + strand, within NIAGMN_20190at 3984.654 kb on - strandat 3984.656 kb on + strandat 3984.678 kb on + strandat 3984.737 kb on + strand, within yfdEat 3984.737 kb on + strand, within yfdEat 3984.737 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.739 kb on + strand, within yfdEat 3984.765 kb on + strand, within yfdEat 3984.870 kb on + strand, within yfdEat 3984.902 kb on - strand, within yfdEat 3984.971 kb on + strand, within yfdEat 3985.021 kb on + strand, within yfdEat 3985.022 kb on - strand, within yfdEat 3985.023 kb on + strand, within yfdEat 3985.023 kb on + strand, within yfdEat 3985.069 kb on + strand, within yfdEat 3985.295 kb on + strand, within yfdE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,982,813 + oxc NIAGMN_20180 0.72 +0.4
3,982,813 + oxc NIAGMN_20180 0.72 +0.0
3,982,930 - oxc NIAGMN_20180 0.79 +1.0
3,982,975 + oxc NIAGMN_20180 0.82 -1.8
3,983,102 + oxc NIAGMN_20180 0.89 +1.0
3,983,200 - -0.8
3,983,658 - yfdV NIAGMN_20185 0.32 +0.7
3,983,838 + yfdV NIAGMN_20185 0.51 +0.5
3,983,879 + yfdV NIAGMN_20185 0.56 +0.0
3,984,202 + yfdV NIAGMN_20185 0.90 +0.0
3,984,239 + +0.8
3,984,274 + +1.0
3,984,436 + NIAGMN_20190 0.28 +0.6
3,984,654 - +0.0
3,984,656 + -0.1
3,984,678 + -2.8
3,984,737 + yfdE NIAGMN_20195 0.12 -1.6
3,984,737 + yfdE NIAGMN_20195 0.12 -0.6
3,984,737 + yfdE NIAGMN_20195 0.12 +0.2
3,984,739 + yfdE NIAGMN_20195 0.12 +0.1
3,984,739 + yfdE NIAGMN_20195 0.12 +1.7
3,984,739 + yfdE NIAGMN_20195 0.12 -0.4
3,984,739 + yfdE NIAGMN_20195 0.12 -0.6
3,984,739 + yfdE NIAGMN_20195 0.12 +0.4
3,984,765 + yfdE NIAGMN_20195 0.15 +0.9
3,984,870 + yfdE NIAGMN_20195 0.27 -3.0
3,984,902 - yfdE NIAGMN_20195 0.30 +0.9
3,984,971 + yfdE NIAGMN_20195 0.38 -1.5
3,985,021 + yfdE NIAGMN_20195 0.44 +1.2
3,985,022 - yfdE NIAGMN_20195 0.44 -0.6
3,985,023 + yfdE NIAGMN_20195 0.44 +2.6
3,985,023 + yfdE NIAGMN_20195 0.44 +0.4
3,985,069 + yfdE NIAGMN_20195 0.49 +0.5
3,985,295 + yfdE NIAGMN_20195 0.75 +2.1

Or see this region's nucleotide sequence