Strain Fitness in Escherichia coli ECRC102 around NIAGMN_19650

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfF and hyfE are separated by 19 nucleotideshyfE and hyfD are separated by 11 nucleotides NIAGMN_19645: hyfF - hydrogenase 4 subunit F, at 3,877,079 to 3,878,644 hyfF NIAGMN_19650: hyfE - hydrogenase 4 membrane subunit, at 3,878,664 to 3,879,314 hyfE NIAGMN_19655: hyfD - hydrogenase 4 subunit HyfD, at 3,879,326 to 3,880,765 hyfD Position (kb) 3878 3879 3880Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3877.729 kb on + strand, within hyfFat 3877.730 kb on - strand, within hyfFat 3877.835 kb on - strand, within hyfFat 3877.842 kb on + strand, within hyfFat 3877.842 kb on + strand, within hyfFat 3877.842 kb on + strand, within hyfFat 3877.842 kb on + strand, within hyfFat 3877.842 kb on + strand, within hyfFat 3877.843 kb on - strand, within hyfFat 3878.009 kb on + strand, within hyfFat 3878.010 kb on - strand, within hyfFat 3878.144 kb on - strand, within hyfFat 3878.144 kb on - strand, within hyfFat 3878.149 kb on + strand, within hyfFat 3878.150 kb on - strand, within hyfFat 3878.158 kb on - strand, within hyfFat 3878.158 kb on - strand, within hyfFat 3878.158 kb on - strand, within hyfFat 3878.158 kb on - strand, within hyfFat 3878.193 kb on + strand, within hyfFat 3878.194 kb on - strand, within hyfFat 3878.194 kb on - strand, within hyfFat 3878.316 kb on + strand, within hyfFat 3878.370 kb on + strand, within hyfFat 3878.447 kb on - strand, within hyfFat 3878.542 kb on + strandat 3878.543 kb on - strandat 3878.546 kb on + strandat 3878.546 kb on + strandat 3878.547 kb on - strandat 3878.547 kb on - strandat 3878.547 kb on - strandat 3878.547 kb on - strandat 3878.547 kb on - strandat 3878.550 kb on - strandat 3878.652 kb on + strandat 3878.652 kb on + strandat 3878.653 kb on - strandat 3878.734 kb on - strand, within hyfEat 3878.851 kb on + strand, within hyfEat 3879.003 kb on + strand, within hyfEat 3879.004 kb on - strand, within hyfEat 3879.004 kb on - strand, within hyfEat 3879.082 kb on + strand, within hyfEat 3879.083 kb on - strand, within hyfEat 3879.083 kb on - strand, within hyfEat 3879.215 kb on - strand, within hyfEat 3879.294 kb on + strandat 3879.303 kb on - strandat 3879.303 kb on - strandat 3879.303 kb on - strandat 3879.303 kb on - strandat 3879.711 kb on + strand, within hyfDat 3879.712 kb on - strand, within hyfDat 3879.728 kb on + strand, within hyfDat 3879.729 kb on - strand, within hyfDat 3879.729 kb on - strand, within hyfDat 3879.729 kb on - strand, within hyfDat 3879.729 kb on - strand, within hyfDat 3879.772 kb on - strand, within hyfDat 3879.897 kb on + strand, within hyfDat 3879.898 kb on - strand, within hyfDat 3879.898 kb on - strand, within hyfDat 3879.898 kb on - strand, within hyfDat 3879.898 kb on - strand, within hyfDat 3880.003 kb on - strand, within hyfDat 3880.147 kb on - strand, within hyfDat 3880.147 kb on - strand, within hyfDat 3880.164 kb on - strand, within hyfDat 3880.228 kb on - strand, within hyfDat 3880.228 kb on - strand, within hyfDat 3880.269 kb on - strand, within hyfDat 3880.299 kb on + strand, within hyfDat 3880.299 kb on + strand, within hyfDat 3880.302 kb on + strand, within hyfD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,877,729 + hyfF NIAGMN_19645 0.42 -0.1
3,877,730 - hyfF NIAGMN_19645 0.42 +0.4
3,877,835 - hyfF NIAGMN_19645 0.48 -0.9
3,877,842 + hyfF NIAGMN_19645 0.49 -1.1
3,877,842 + hyfF NIAGMN_19645 0.49 +1.2
3,877,842 + hyfF NIAGMN_19645 0.49 -0.1
3,877,842 + hyfF NIAGMN_19645 0.49 -0.2
3,877,842 + hyfF NIAGMN_19645 0.49 -0.8
3,877,843 - hyfF NIAGMN_19645 0.49 +0.9
3,878,009 + hyfF NIAGMN_19645 0.59 -0.3
3,878,010 - hyfF NIAGMN_19645 0.59 +2.3
3,878,144 - hyfF NIAGMN_19645 0.68 +2.2
3,878,144 - hyfF NIAGMN_19645 0.68 +0.1
3,878,149 + hyfF NIAGMN_19645 0.68 -0.4
3,878,150 - hyfF NIAGMN_19645 0.68 -0.4
3,878,158 - hyfF NIAGMN_19645 0.69 -2.2
3,878,158 - hyfF NIAGMN_19645 0.69 -2.1
3,878,158 - hyfF NIAGMN_19645 0.69 -0.2
3,878,158 - hyfF NIAGMN_19645 0.69 -0.0
3,878,193 + hyfF NIAGMN_19645 0.71 -1.6
3,878,194 - hyfF NIAGMN_19645 0.71 -2.5
3,878,194 - hyfF NIAGMN_19645 0.71 +0.3
3,878,316 + hyfF NIAGMN_19645 0.79 +4.0
3,878,370 + hyfF NIAGMN_19645 0.82 -1.4
3,878,447 - hyfF NIAGMN_19645 0.87 +2.8
3,878,542 + +0.4
3,878,543 - -0.6
3,878,546 + +0.8
3,878,546 + -1.9
3,878,547 - +0.6
3,878,547 - +1.1
3,878,547 - -0.5
3,878,547 - +0.1
3,878,547 - -3.1
3,878,550 - +0.1
3,878,652 + +3.2
3,878,652 + +1.1
3,878,653 - -0.4
3,878,734 - hyfE NIAGMN_19650 0.11 -0.3
3,878,851 + hyfE NIAGMN_19650 0.29 +0.5
3,879,003 + hyfE NIAGMN_19650 0.52 +2.3
3,879,004 - hyfE NIAGMN_19650 0.52 +1.8
3,879,004 - hyfE NIAGMN_19650 0.52 +1.8
3,879,082 + hyfE NIAGMN_19650 0.64 +0.8
3,879,083 - hyfE NIAGMN_19650 0.64 +0.7
3,879,083 - hyfE NIAGMN_19650 0.64 +3.0
3,879,215 - hyfE NIAGMN_19650 0.85 +0.8
3,879,294 + -2.4
3,879,303 - -2.2
3,879,303 - +1.8
3,879,303 - -1.2
3,879,303 - -2.3
3,879,711 + hyfD NIAGMN_19655 0.27 -0.5
3,879,712 - hyfD NIAGMN_19655 0.27 +0.0
3,879,728 + hyfD NIAGMN_19655 0.28 +0.8
3,879,729 - hyfD NIAGMN_19655 0.28 +2.7
3,879,729 - hyfD NIAGMN_19655 0.28 -0.7
3,879,729 - hyfD NIAGMN_19655 0.28 -0.0
3,879,729 - hyfD NIAGMN_19655 0.28 -2.7
3,879,772 - hyfD NIAGMN_19655 0.31 -0.8
3,879,897 + hyfD NIAGMN_19655 0.40 -0.7
3,879,898 - hyfD NIAGMN_19655 0.40 +1.7
3,879,898 - hyfD NIAGMN_19655 0.40 -0.1
3,879,898 - hyfD NIAGMN_19655 0.40 +0.0
3,879,898 - hyfD NIAGMN_19655 0.40 -0.4
3,880,003 - hyfD NIAGMN_19655 0.47 +3.4
3,880,147 - hyfD NIAGMN_19655 0.57 -0.7
3,880,147 - hyfD NIAGMN_19655 0.57 -0.5
3,880,164 - hyfD NIAGMN_19655 0.58 +2.1
3,880,228 - hyfD NIAGMN_19655 0.63 -1.0
3,880,228 - hyfD NIAGMN_19655 0.63 -1.5
3,880,269 - hyfD NIAGMN_19655 0.65 -0.8
3,880,299 + hyfD NIAGMN_19655 0.68 +0.4
3,880,299 + hyfD NIAGMN_19655 0.68 -1.4
3,880,302 + hyfD NIAGMN_19655 0.68 +1.1

Or see this region's nucleotide sequence