Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gutQ and srlR overlap by 8 nucleotides srlR and gutM are separated by 66 nucleotides gutM and srlD are separated by 105 nucleotides srlD and srlB are separated by 3 nucleotides
NIAGMN_18425: gutQ - arabinose-5-phosphate isomerase GutQ, at 3,646,967 to 3,647,932
gutQ
NIAGMN_18430: srlR - glucitol operon DNA-binding transcriptional repressor SrlR, at 3,647,925 to 3,648,698
srlR
NIAGMN_18435: gutM - transcriptional regulator GutM, at 3,648,765 to 3,649,124
gutM
NIAGMN_18440: srlD - sorbitol-6-phosphate dehydrogenase, at 3,649,230 to 3,650,009
srlD
NIAGMN_18445: srlB - PTS glucitol/sorbitol transporter subunit IIA, at 3,650,013 to 3,650,384
srlB
Position (kb)
3648
3649
3650 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3647.918 kb on + strand at 3647.919 kb on - strand at 3647.947 kb on - strand at 3648.045 kb on + strand, within srlR at 3648.045 kb on + strand, within srlR at 3648.045 kb on + strand, within srlR at 3648.046 kb on - strand, within srlR at 3648.046 kb on - strand, within srlR at 3648.046 kb on - strand, within srlR at 3648.122 kb on + strand, within srlR at 3648.133 kb on + strand, within srlR at 3648.133 kb on + strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.134 kb on - strand, within srlR at 3648.287 kb on + strand, within srlR at 3648.357 kb on - strand, within srlR at 3648.359 kb on + strand, within srlR at 3648.435 kb on - strand, within srlR at 3648.435 kb on - strand, within srlR at 3648.435 kb on - strand, within srlR at 3648.456 kb on + strand, within srlR at 3648.456 kb on + strand, within srlR at 3648.491 kb on + strand, within srlR at 3648.542 kb on - strand, within srlR at 3648.542 kb on - strand, within srlR at 3648.545 kb on + strand, within srlR at 3648.546 kb on - strand, within srlR at 3648.546 kb on - strand, within srlR at 3648.546 kb on - strand, within srlR at 3648.546 kb on - strand, within srlR at 3648.594 kb on - strand, within srlR at 3648.594 kb on - strand, within srlR at 3648.662 kb on - strand at 3648.662 kb on - strand at 3648.766 kb on + strand at 3648.796 kb on - strand at 3648.866 kb on + strand, within gutM at 3648.866 kb on + strand, within gutM at 3648.866 kb on + strand, within gutM at 3648.866 kb on + strand, within gutM at 3648.867 kb on - strand, within gutM at 3648.915 kb on - strand, within gutM at 3649.023 kb on + strand, within gutM at 3649.023 kb on + strand, within gutM at 3649.024 kb on - strand, within gutM at 3649.024 kb on - strand, within gutM at 3649.024 kb on - strand, within gutM at 3649.024 kb on - strand, within gutM at 3649.124 kb on + strand at 3649.124 kb on + strand at 3649.125 kb on - strand at 3649.135 kb on + strand at 3649.136 kb on - strand at 3649.140 kb on + strand at 3649.141 kb on - strand at 3649.157 kb on + strand at 3649.157 kb on + strand at 3649.158 kb on - strand at 3649.189 kb on + strand at 3649.190 kb on - strand at 3649.190 kb on - strand at 3649.193 kb on - strand at 3649.193 kb on - strand at 3649.289 kb on + strand at 3649.300 kb on + strand at 3649.301 kb on - strand at 3649.301 kb on - strand at 3649.301 kb on - strand at 3649.314 kb on - strand, within srlD at 3649.314 kb on - strand, within srlD at 3649.314 kb on - strand, within srlD at 3649.366 kb on + strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.367 kb on - strand, within srlD at 3649.369 kb on + strand, within srlD at 3649.369 kb on + strand, within srlD at 3649.412 kb on - strand, within srlD at 3649.479 kb on - strand, within srlD at 3649.548 kb on - strand, within srlD at 3649.664 kb on - strand, within srlD at 3649.750 kb on + strand, within srlD at 3649.751 kb on - strand, within srlD at 3649.751 kb on - strand, within srlD at 3649.759 kb on - strand, within srlD at 3649.786 kb on - strand, within srlD at 3649.843 kb on + strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.844 kb on - strand, within srlD at 3649.968 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,647,918 + +2.1 3,647,919 - +2.0 3,647,947 - +3.0 3,648,045 + srlR NIAGMN_18430 0.16 +1.9 3,648,045 + srlR NIAGMN_18430 0.16 -2.3 3,648,045 + srlR NIAGMN_18430 0.16 -2.9 3,648,046 - srlR NIAGMN_18430 0.16 +0.5 3,648,046 - srlR NIAGMN_18430 0.16 -0.0 3,648,046 - srlR NIAGMN_18430 0.16 +0.2 3,648,122 + srlR NIAGMN_18430 0.25 +0.2 3,648,133 + srlR NIAGMN_18430 0.27 -0.3 3,648,133 + srlR NIAGMN_18430 0.27 -1.1 3,648,134 - srlR NIAGMN_18430 0.27 +1.1 3,648,134 - srlR NIAGMN_18430 0.27 -0.7 3,648,134 - srlR NIAGMN_18430 0.27 -1.1 3,648,134 - srlR NIAGMN_18430 0.27 -2.3 3,648,134 - srlR NIAGMN_18430 0.27 -1.0 3,648,134 - srlR NIAGMN_18430 0.27 -0.3 3,648,134 - srlR NIAGMN_18430 0.27 -3.5 3,648,134 - srlR NIAGMN_18430 0.27 -0.5 3,648,287 + srlR NIAGMN_18430 0.47 +0.0 3,648,357 - srlR NIAGMN_18430 0.56 -3.4 3,648,359 + srlR NIAGMN_18430 0.56 +2.9 3,648,435 - srlR NIAGMN_18430 0.66 -0.8 3,648,435 - srlR NIAGMN_18430 0.66 -2.3 3,648,435 - srlR NIAGMN_18430 0.66 -0.1 3,648,456 + srlR NIAGMN_18430 0.69 -2.2 3,648,456 + srlR NIAGMN_18430 0.69 -0.3 3,648,491 + srlR NIAGMN_18430 0.73 +1.0 3,648,542 - srlR NIAGMN_18430 0.80 -0.1 3,648,542 - srlR NIAGMN_18430 0.80 -0.4 3,648,545 + srlR NIAGMN_18430 0.80 -1.3 3,648,546 - srlR NIAGMN_18430 0.80 +2.9 3,648,546 - srlR NIAGMN_18430 0.80 -0.6 3,648,546 - srlR NIAGMN_18430 0.80 -1.7 3,648,546 - srlR NIAGMN_18430 0.80 -3.4 3,648,594 - srlR NIAGMN_18430 0.86 -1.4 3,648,594 - srlR NIAGMN_18430 0.86 -1.9 3,648,662 - +1.2 3,648,662 - +0.0 3,648,766 + -3.9 3,648,796 - -1.6 3,648,866 + gutM NIAGMN_18435 0.28 -1.9 3,648,866 + gutM NIAGMN_18435 0.28 +0.4 3,648,866 + gutM NIAGMN_18435 0.28 +0.9 3,648,866 + gutM NIAGMN_18435 0.28 -0.3 3,648,867 - gutM NIAGMN_18435 0.28 +0.8 3,648,915 - gutM NIAGMN_18435 0.42 -1.0 3,649,023 + gutM NIAGMN_18435 0.72 -1.1 3,649,023 + gutM NIAGMN_18435 0.72 +2.0 3,649,024 - gutM NIAGMN_18435 0.72 -1.5 3,649,024 - gutM NIAGMN_18435 0.72 -1.5 3,649,024 - gutM NIAGMN_18435 0.72 -0.4 3,649,024 - gutM NIAGMN_18435 0.72 +0.5 3,649,124 + +1.8 3,649,124 + -0.7 3,649,125 - +0.1 3,649,135 + -0.5 3,649,136 - +0.7 3,649,140 + +0.0 3,649,141 - +1.0 3,649,157 + -0.2 3,649,157 + -0.3 3,649,158 - +2.0 3,649,189 + +0.2 3,649,190 - -1.0 3,649,190 - +1.5 3,649,193 - -1.3 3,649,193 - +0.8 3,649,289 + -0.2 3,649,300 + +0.7 3,649,301 - -2.0 3,649,301 - -1.3 3,649,301 - +0.9 3,649,314 - srlD NIAGMN_18440 0.11 -3.4 3,649,314 - srlD NIAGMN_18440 0.11 +1.3 3,649,314 - srlD NIAGMN_18440 0.11 +0.8 3,649,366 + srlD NIAGMN_18440 0.17 -1.5 3,649,367 - srlD NIAGMN_18440 0.18 +0.7 3,649,367 - srlD NIAGMN_18440 0.18 +2.6 3,649,367 - srlD NIAGMN_18440 0.18 -3.5 3,649,367 - srlD NIAGMN_18440 0.18 -2.1 3,649,367 - srlD NIAGMN_18440 0.18 -3.3 3,649,367 - srlD NIAGMN_18440 0.18 +1.8 3,649,367 - srlD NIAGMN_18440 0.18 +1.2 3,649,369 + srlD NIAGMN_18440 0.18 +2.0 3,649,369 + srlD NIAGMN_18440 0.18 +3.3 3,649,412 - srlD NIAGMN_18440 0.23 -2.1 3,649,479 - srlD NIAGMN_18440 0.32 +0.1 3,649,548 - srlD NIAGMN_18440 0.41 +2.8 3,649,664 - srlD NIAGMN_18440 0.56 -2.2 3,649,750 + srlD NIAGMN_18440 0.67 -0.7 3,649,751 - srlD NIAGMN_18440 0.67 +2.8 3,649,751 - srlD NIAGMN_18440 0.67 +1.6 3,649,759 - srlD NIAGMN_18440 0.68 -1.1 3,649,786 - srlD NIAGMN_18440 0.71 +0.6 3,649,843 + srlD NIAGMN_18440 0.79 -1.3 3,649,844 - srlD NIAGMN_18440 0.79 -1.6 3,649,844 - srlD NIAGMN_18440 0.79 -1.0 3,649,844 - srlD NIAGMN_18440 0.79 +0.6 3,649,844 - srlD NIAGMN_18440 0.79 +0.7 3,649,968 + -0.8
Or see this region's nucleotide sequence