Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoD and dnaG are separated by 194 nucleotides dnaG and rpsU are separated by 110 nucleotides rpsU and tsaD are separated by 237 nucleotides
NIAGMN_16420: rpoD - RNA polymerase sigma factor RpoD, at 3,247,310 to 3,249,151
rpoD
NIAGMN_16425: dnaG - DNA primase, at 3,249,346 to 3,251,091
dnaG
NIAGMN_16430: rpsU - 30S ribosomal protein S21, at 3,251,202 to 3,251,417
rpsU
NIAGMN_16435: tsaD - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 3,251,655 to 3,252,668
tsaD
Position (kb)
3249
3250
3251
3252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3249.259 kb on - strand at 3249.273 kb on - strand at 3249.273 kb on - strand at 3249.273 kb on - strand at 3249.273 kb on - strand at 3249.275 kb on - strand at 3249.275 kb on - strand at 3249.275 kb on - strand at 3249.277 kb on - strand at 3249.277 kb on - strand at 3249.277 kb on - strand at 3249.335 kb on - strand at 3249.335 kb on - strand at 3249.335 kb on - strand at 3251.092 kb on - strand at 3251.116 kb on - strand at 3251.547 kb on + strand at 3251.548 kb on - strand at 3251.548 kb on - strand at 3251.550 kb on - strand at 3251.562 kb on + strand at 3251.562 kb on + strand at 3251.562 kb on + strand at 3251.562 kb on + strand at 3251.563 kb on - strand at 3251.564 kb on + strand at 3251.564 kb on + strand at 3251.565 kb on - strand at 3251.565 kb on - strand at 3251.565 kb on - strand at 3251.565 kb on - strand at 3251.598 kb on + strand at 3251.598 kb on + strand at 3251.619 kb on + strand at 3251.631 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,249,259 - -1.6 3,249,273 - -2.0 3,249,273 - +0.4 3,249,273 - -2.0 3,249,273 - -1.0 3,249,275 - -0.5 3,249,275 - -0.4 3,249,275 - -2.4 3,249,277 - -2.7 3,249,277 - -1.5 3,249,277 - -2.4 3,249,335 - -0.5 3,249,335 - +0.0 3,249,335 - -2.7 3,251,092 - -0.9 3,251,116 - +0.6 3,251,547 + -0.4 3,251,548 - +0.9 3,251,548 - -2.6 3,251,550 - -0.4 3,251,562 + +2.0 3,251,562 + -3.2 3,251,562 + -0.9 3,251,562 + -3.2 3,251,563 - -0.0 3,251,564 + -0.5 3,251,564 + -0.4 3,251,565 - -2.4 3,251,565 - +0.4 3,251,565 - +0.5 3,251,565 - -0.3 3,251,598 + +0.3 3,251,598 + +2.2 3,251,619 + -1.0 3,251,631 + +0.1
Or see this region's nucleotide sequence