Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mlaE and mlaD are separated by 4 nucleotides mlaD and mlaC are separated by 18 nucleotides mlaC and mlaC overlap by 1 nucleotides mlaC and ibaG are separated by 144 nucleotides ibaG and murA are separated by 54 nucleotides
NIAGMN_15740: mlaE - lipid asymmetry maintenance ABC transporter permease subunit MlaE, at 3,120,128 to 3,120,910
mlaE
NIAGMN_15745: mlaD - outer membrane lipid asymmetry maintenance protein MlaD, at 3,120,915 to 3,121,466
mlaD
NIAGMN_15750: mlaC - phospholipid-binding protein MlaC, at 3,121,485 to 3,122,120
mlaC
NIAGMN_15755: mlaC - phospholipid-binding protein MlaC, at 3,122,120 to 3,122,413
mlaC
NIAGMN_15760: ibaG - BolA family iron metabolism protein IbaG, at 3,122,558 to 3,122,827
ibaG
NIAGMN_15765: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 3,122,882 to 3,124,141
murA
Position (kb)
3121
3122
3123 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3120.520 kb on + strand, within mlaE at 3120.521 kb on - strand, within mlaE at 3120.556 kb on + strand, within mlaE at 3120.608 kb on - strand, within mlaE at 3120.715 kb on + strand, within mlaE at 3120.715 kb on + strand, within mlaE at 3120.739 kb on + strand, within mlaE at 3120.740 kb on - strand, within mlaE at 3120.947 kb on + strand at 3120.947 kb on + strand at 3121.015 kb on + strand, within mlaD at 3121.015 kb on + strand, within mlaD at 3121.015 kb on + strand, within mlaD at 3121.015 kb on + strand, within mlaD at 3121.016 kb on - strand, within mlaD at 3121.016 kb on - strand, within mlaD at 3121.032 kb on + strand, within mlaD at 3121.041 kb on + strand, within mlaD at 3121.091 kb on + strand, within mlaD at 3121.124 kb on + strand, within mlaD at 3121.146 kb on + strand, within mlaD at 3121.147 kb on - strand, within mlaD at 3121.147 kb on - strand, within mlaD at 3121.147 kb on - strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.182 kb on + strand, within mlaD at 3121.183 kb on - strand, within mlaD at 3121.183 kb on - strand, within mlaD at 3121.183 kb on - strand, within mlaD at 3121.184 kb on + strand, within mlaD at 3121.184 kb on + strand, within mlaD at 3121.185 kb on - strand, within mlaD at 3121.190 kb on + strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.191 kb on - strand, within mlaD at 3121.245 kb on + strand, within mlaD at 3121.246 kb on - strand, within mlaD at 3121.246 kb on - strand, within mlaD at 3121.246 kb on - strand, within mlaD at 3121.255 kb on + strand, within mlaD at 3121.292 kb on + strand, within mlaD at 3121.293 kb on - strand, within mlaD at 3121.374 kb on - strand, within mlaD at 3121.377 kb on - strand, within mlaD at 3121.377 kb on - strand, within mlaD at 3121.377 kb on - strand, within mlaD at 3121.391 kb on + strand, within mlaD at 3121.397 kb on + strand, within mlaD at 3121.398 kb on - strand, within mlaD at 3121.430 kb on + strand at 3121.430 kb on + strand at 3121.431 kb on - strand at 3121.431 kb on - strand at 3121.431 kb on - strand at 3121.498 kb on + strand at 3121.498 kb on + strand at 3121.499 kb on - strand at 3121.566 kb on + strand, within mlaC at 3121.567 kb on - strand, within mlaC at 3121.568 kb on + strand, within mlaC at 3121.568 kb on + strand, within mlaC at 3121.568 kb on + strand, within mlaC at 3121.569 kb on - strand, within mlaC at 3121.647 kb on + strand, within mlaC at 3121.655 kb on + strand, within mlaC at 3121.655 kb on + strand, within mlaC at 3121.683 kb on + strand, within mlaC at 3121.683 kb on + strand, within mlaC at 3121.683 kb on + strand, within mlaC at 3121.684 kb on - strand, within mlaC at 3121.684 kb on - strand, within mlaC at 3121.687 kb on + strand, within mlaC at 3121.687 kb on + strand, within mlaC at 3121.687 kb on + strand, within mlaC at 3121.725 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.728 kb on + strand, within mlaC at 3121.729 kb on - strand, within mlaC at 3121.729 kb on - strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.761 kb on + strand, within mlaC at 3121.762 kb on - strand, within mlaC at 3121.762 kb on - strand, within mlaC at 3121.782 kb on + strand, within mlaC at 3121.797 kb on + strand, within mlaC at 3121.797 kb on + strand, within mlaC at 3121.818 kb on + strand, within mlaC at 3121.818 kb on + strand, within mlaC at 3121.818 kb on + strand, within mlaC at 3121.818 kb on + strand, within mlaC at 3121.818 kb on + strand, within mlaC at 3121.819 kb on - strand, within mlaC at 3121.819 kb on - strand, within mlaC at 3121.819 kb on - strand, within mlaC at 3121.865 kb on + strand, within mlaC at 3121.895 kb on + strand, within mlaC at 3121.895 kb on + strand, within mlaC at 3121.974 kb on + strand, within mlaC at 3121.974 kb on + strand, within mlaC at 3122.000 kb on + strand, within mlaC at 3122.038 kb on + strand, within mlaC at 3122.039 kb on - strand, within mlaC at 3122.042 kb on + strand, within mlaC at 3122.042 kb on + strand, within mlaC at 3122.042 kb on + strand, within mlaC at 3122.043 kb on - strand, within mlaC at 3122.043 kb on - strand, within mlaC at 3122.043 kb on - strand, within mlaC at 3122.052 kb on - strand, within mlaC at 3122.236 kb on + strand, within mlaC at 3122.237 kb on - strand, within mlaC at 3122.273 kb on - strand, within mlaC at 3122.363 kb on + strand, within mlaC at 3122.363 kb on + strand, within mlaC at 3122.363 kb on + strand, within mlaC at 3122.364 kb on - strand, within mlaC at 3122.364 kb on - strand, within mlaC at 3122.364 kb on - strand, within mlaC at 3122.364 kb on - strand, within mlaC at 3122.366 kb on - strand, within mlaC at 3122.431 kb on - strand at 3122.506 kb on + strand at 3122.506 kb on + strand at 3122.506 kb on + strand at 3122.507 kb on - strand at 3122.507 kb on - strand at 3122.510 kb on + strand at 3122.510 kb on + strand at 3122.549 kb on + strand at 3122.552 kb on + strand at 3122.552 kb on + strand at 3122.552 kb on + strand at 3122.720 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.738 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.780 kb on + strand, within ibaG at 3122.782 kb on + strand, within ibaG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,120,520 + mlaE NIAGMN_15740 0.50 -0.9 3,120,521 - mlaE NIAGMN_15740 0.50 +2.8 3,120,556 + mlaE NIAGMN_15740 0.55 -2.2 3,120,608 - mlaE NIAGMN_15740 0.61 +0.3 3,120,715 + mlaE NIAGMN_15740 0.75 +2.0 3,120,715 + mlaE NIAGMN_15740 0.75 -2.4 3,120,739 + mlaE NIAGMN_15740 0.78 -1.0 3,120,740 - mlaE NIAGMN_15740 0.78 -0.7 3,120,947 + +0.4 3,120,947 + +1.1 3,121,015 + mlaD NIAGMN_15745 0.18 +1.2 3,121,015 + mlaD NIAGMN_15745 0.18 +0.8 3,121,015 + mlaD NIAGMN_15745 0.18 -1.5 3,121,015 + mlaD NIAGMN_15745 0.18 -1.5 3,121,016 - mlaD NIAGMN_15745 0.18 -1.3 3,121,016 - mlaD NIAGMN_15745 0.18 +1.1 3,121,032 + mlaD NIAGMN_15745 0.21 +0.5 3,121,041 + mlaD NIAGMN_15745 0.23 -1.9 3,121,091 + mlaD NIAGMN_15745 0.32 -0.7 3,121,124 + mlaD NIAGMN_15745 0.38 -1.1 3,121,146 + mlaD NIAGMN_15745 0.42 +0.4 3,121,147 - mlaD NIAGMN_15745 0.42 +0.2 3,121,147 - mlaD NIAGMN_15745 0.42 -0.3 3,121,147 - mlaD NIAGMN_15745 0.42 -0.5 3,121,182 + mlaD NIAGMN_15745 0.48 +0.5 3,121,182 + mlaD NIAGMN_15745 0.48 -2.2 3,121,182 + mlaD NIAGMN_15745 0.48 +0.1 3,121,182 + mlaD NIAGMN_15745 0.48 -0.9 3,121,182 + mlaD NIAGMN_15745 0.48 -0.3 3,121,182 + mlaD NIAGMN_15745 0.48 +1.7 3,121,182 + mlaD NIAGMN_15745 0.48 -0.2 3,121,183 - mlaD NIAGMN_15745 0.49 +1.0 3,121,183 - mlaD NIAGMN_15745 0.49 +1.0 3,121,183 - mlaD NIAGMN_15745 0.49 +0.5 3,121,184 + mlaD NIAGMN_15745 0.49 +0.8 3,121,184 + mlaD NIAGMN_15745 0.49 +0.4 3,121,185 - mlaD NIAGMN_15745 0.49 -0.5 3,121,190 + mlaD NIAGMN_15745 0.50 +1.8 3,121,191 - mlaD NIAGMN_15745 0.50 -0.7 3,121,191 - mlaD NIAGMN_15745 0.50 -1.1 3,121,191 - mlaD NIAGMN_15745 0.50 +1.6 3,121,191 - mlaD NIAGMN_15745 0.50 -0.3 3,121,191 - mlaD NIAGMN_15745 0.50 -0.2 3,121,191 - mlaD NIAGMN_15745 0.50 +0.8 3,121,245 + mlaD NIAGMN_15745 0.60 +0.4 3,121,246 - mlaD NIAGMN_15745 0.60 -0.1 3,121,246 - mlaD NIAGMN_15745 0.60 +0.9 3,121,246 - mlaD NIAGMN_15745 0.60 +3.0 3,121,255 + mlaD NIAGMN_15745 0.62 +0.4 3,121,292 + mlaD NIAGMN_15745 0.68 +2.5 3,121,293 - mlaD NIAGMN_15745 0.68 -3.4 3,121,374 - mlaD NIAGMN_15745 0.83 +1.3 3,121,377 - mlaD NIAGMN_15745 0.84 +0.9 3,121,377 - mlaD NIAGMN_15745 0.84 -1.1 3,121,377 - mlaD NIAGMN_15745 0.84 +1.3 3,121,391 + mlaD NIAGMN_15745 0.86 +1.1 3,121,397 + mlaD NIAGMN_15745 0.87 +1.8 3,121,398 - mlaD NIAGMN_15745 0.88 +0.6 3,121,430 + -2.5 3,121,430 + +0.2 3,121,431 - -2.9 3,121,431 - -0.3 3,121,431 - -0.9 3,121,498 + +1.0 3,121,498 + +0.3 3,121,499 - +2.5 3,121,566 + mlaC NIAGMN_15750 0.13 +0.3 3,121,567 - mlaC NIAGMN_15750 0.13 +1.0 3,121,568 + mlaC NIAGMN_15750 0.13 +2.0 3,121,568 + mlaC NIAGMN_15750 0.13 +1.1 3,121,568 + mlaC NIAGMN_15750 0.13 -1.2 3,121,569 - mlaC NIAGMN_15750 0.13 +1.5 3,121,647 + mlaC NIAGMN_15750 0.25 -2.0 3,121,655 + mlaC NIAGMN_15750 0.27 +1.0 3,121,655 + mlaC NIAGMN_15750 0.27 -1.9 3,121,683 + mlaC NIAGMN_15750 0.31 -0.5 3,121,683 + mlaC NIAGMN_15750 0.31 +0.8 3,121,683 + mlaC NIAGMN_15750 0.31 -0.3 3,121,684 - mlaC NIAGMN_15750 0.31 -0.1 3,121,684 - mlaC NIAGMN_15750 0.31 +2.4 3,121,687 + mlaC NIAGMN_15750 0.32 +0.1 3,121,687 + mlaC NIAGMN_15750 0.32 +1.1 3,121,687 + mlaC NIAGMN_15750 0.32 -2.2 3,121,725 + mlaC NIAGMN_15750 0.38 -1.2 3,121,728 + mlaC NIAGMN_15750 0.38 -2.4 3,121,728 + mlaC NIAGMN_15750 0.38 +0.7 3,121,728 + mlaC NIAGMN_15750 0.38 +2.0 3,121,728 + mlaC NIAGMN_15750 0.38 +1.0 3,121,728 + mlaC NIAGMN_15750 0.38 +0.5 3,121,728 + mlaC NIAGMN_15750 0.38 +2.0 3,121,728 + mlaC NIAGMN_15750 0.38 +1.8 3,121,729 - mlaC NIAGMN_15750 0.38 +0.1 3,121,729 - mlaC NIAGMN_15750 0.38 +1.5 3,121,761 + mlaC NIAGMN_15750 0.43 -1.2 3,121,761 + mlaC NIAGMN_15750 0.43 -1.1 3,121,761 + mlaC NIAGMN_15750 0.43 -0.1 3,121,761 + mlaC NIAGMN_15750 0.43 -0.1 3,121,761 + mlaC NIAGMN_15750 0.43 +1.7 3,121,761 + mlaC NIAGMN_15750 0.43 -0.4 3,121,762 - mlaC NIAGMN_15750 0.44 -1.7 3,121,762 - mlaC NIAGMN_15750 0.44 -2.0 3,121,782 + mlaC NIAGMN_15750 0.47 -3.1 3,121,797 + mlaC NIAGMN_15750 0.49 +1.4 3,121,797 + mlaC NIAGMN_15750 0.49 -1.4 3,121,818 + mlaC NIAGMN_15750 0.52 -0.0 3,121,818 + mlaC NIAGMN_15750 0.52 +0.8 3,121,818 + mlaC NIAGMN_15750 0.52 +0.1 3,121,818 + mlaC NIAGMN_15750 0.52 -1.3 3,121,818 + mlaC NIAGMN_15750 0.52 -0.3 3,121,819 - mlaC NIAGMN_15750 0.53 +1.6 3,121,819 - mlaC NIAGMN_15750 0.53 -0.1 3,121,819 - mlaC NIAGMN_15750 0.53 +0.4 3,121,865 + mlaC NIAGMN_15750 0.60 +0.7 3,121,895 + mlaC NIAGMN_15750 0.64 -0.5 3,121,895 + mlaC NIAGMN_15750 0.64 +0.4 3,121,974 + mlaC NIAGMN_15750 0.77 -0.2 3,121,974 + mlaC NIAGMN_15750 0.77 -1.5 3,122,000 + mlaC NIAGMN_15750 0.81 -0.7 3,122,038 + mlaC NIAGMN_15750 0.87 +0.5 3,122,039 - mlaC NIAGMN_15750 0.87 -2.2 3,122,042 + mlaC NIAGMN_15750 0.88 +0.3 3,122,042 + mlaC NIAGMN_15750 0.88 -1.8 3,122,042 + mlaC NIAGMN_15750 0.88 +0.4 3,122,043 - mlaC NIAGMN_15750 0.88 +0.4 3,122,043 - mlaC NIAGMN_15750 0.88 -1.0 3,122,043 - mlaC NIAGMN_15750 0.88 +0.3 3,122,052 - mlaC NIAGMN_15750 0.89 +1.5 3,122,236 + mlaC NIAGMN_15755 0.39 -0.0 3,122,237 - mlaC NIAGMN_15755 0.40 +0.8 3,122,273 - mlaC NIAGMN_15755 0.52 -0.2 3,122,363 + mlaC NIAGMN_15755 0.83 +1.1 3,122,363 + mlaC NIAGMN_15755 0.83 +0.8 3,122,363 + mlaC NIAGMN_15755 0.83 -0.2 3,122,364 - mlaC NIAGMN_15755 0.83 -0.3 3,122,364 - mlaC NIAGMN_15755 0.83 +1.4 3,122,364 - mlaC NIAGMN_15755 0.83 -3.0 3,122,364 - mlaC NIAGMN_15755 0.83 +0.7 3,122,366 - mlaC NIAGMN_15755 0.84 +0.8 3,122,431 - +1.1 3,122,506 + -1.5 3,122,506 + -0.4 3,122,506 + -0.8 3,122,507 - +1.8 3,122,507 - +0.3 3,122,510 + +0.6 3,122,510 + -4.0 3,122,549 + +0.2 3,122,552 + +1.1 3,122,552 + +0.7 3,122,552 + -2.5 3,122,720 + ibaG NIAGMN_15760 0.60 -1.9 3,122,738 + ibaG NIAGMN_15760 0.67 +1.8 3,122,738 + ibaG NIAGMN_15760 0.67 -1.7 3,122,738 + ibaG NIAGMN_15760 0.67 -2.3 3,122,738 + ibaG NIAGMN_15760 0.67 -2.7 3,122,738 + ibaG NIAGMN_15760 0.67 -0.8 3,122,738 + ibaG NIAGMN_15760 0.67 +0.1 3,122,738 + ibaG NIAGMN_15760 0.67 -0.5 3,122,738 + ibaG NIAGMN_15760 0.67 -1.7 3,122,780 + ibaG NIAGMN_15760 0.82 -1.4 3,122,780 + ibaG NIAGMN_15760 0.82 -1.1 3,122,780 + ibaG NIAGMN_15760 0.82 +0.2 3,122,780 + ibaG NIAGMN_15760 0.82 -0.7 3,122,780 + ibaG NIAGMN_15760 0.82 -0.0 3,122,780 + ibaG NIAGMN_15760 0.82 -2.9 3,122,780 + ibaG NIAGMN_15760 0.82 -0.9 3,122,780 + ibaG NIAGMN_15760 0.82 -0.8 3,122,782 + ibaG NIAGMN_15760 0.83 -1.5
Or see this region's nucleotide sequence