Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt elbB and mtgA overlap by 4 nucleotides mtgA and yrbL overlap by 4 nucleotides yrbL and npr are separated by 213 nucleotides npr and rapZ overlap by 4 nucleotides
NIAGMN_15665: elbB - isoprenoid biosynthesis glyoxalase ElbB, at 3,108,917 to 3,109,570
elbB
NIAGMN_15670: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase, at 3,109,567 to 3,110,295
mtgA
NIAGMN_15675: yrbL - PhoP regulatory network protein YrbL, at 3,110,292 to 3,110,924
yrbL
NIAGMN_15680: npr - PTS phosphocarrier protein NPr, at 3,111,138 to 3,111,410
npr
NIAGMN_15685: rapZ - RNase adapter RapZ, at 3,111,407 to 3,112,261
rapZ
Position (kb)
3110
3111 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3109.321 kb on + strand, within elbB at 3109.321 kb on + strand, within elbB at 3109.321 kb on + strand, within elbB at 3109.321 kb on + strand, within elbB at 3109.321 kb on + strand, within elbB at 3109.322 kb on - strand, within elbB at 3109.378 kb on + strand, within elbB at 3109.468 kb on + strand, within elbB at 3109.469 kb on - strand, within elbB at 3109.490 kb on + strand, within elbB at 3109.490 kb on + strand, within elbB at 3109.491 kb on - strand, within elbB at 3109.491 kb on - strand, within elbB at 3109.491 kb on - strand, within elbB at 3109.491 kb on - strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.492 kb on + strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.493 kb on - strand, within elbB at 3109.534 kb on + strand at 3109.534 kb on + strand at 3109.534 kb on + strand at 3109.534 kb on + strand at 3109.535 kb on - strand at 3109.535 kb on - strand at 3109.593 kb on + strand at 3109.593 kb on + strand at 3109.593 kb on + strand at 3109.668 kb on + strand, within mtgA at 3109.734 kb on + strand, within mtgA at 3109.744 kb on + strand, within mtgA at 3109.745 kb on - strand, within mtgA at 3110.009 kb on + strand, within mtgA at 3110.009 kb on + strand, within mtgA at 3110.107 kb on + strand, within mtgA at 3110.108 kb on - strand, within mtgA at 3110.108 kb on - strand, within mtgA at 3110.133 kb on + strand, within mtgA at 3110.134 kb on - strand, within mtgA at 3110.134 kb on - strand, within mtgA at 3110.154 kb on - strand, within mtgA at 3110.165 kb on + strand, within mtgA at 3110.166 kb on - strand, within mtgA at 3110.166 kb on - strand, within mtgA at 3110.166 kb on - strand, within mtgA at 3110.168 kb on + strand, within mtgA at 3110.168 kb on + strand, within mtgA at 3110.168 kb on + strand, within mtgA at 3110.168 kb on + strand, within mtgA at 3110.169 kb on - strand, within mtgA at 3110.169 kb on - strand, within mtgA at 3110.169 kb on - strand, within mtgA at 3110.169 kb on - strand, within mtgA at 3110.169 kb on - strand, within mtgA at 3110.173 kb on - strand, within mtgA at 3110.173 kb on - strand, within mtgA at 3110.173 kb on - strand, within mtgA at 3110.248 kb on + strand at 3110.248 kb on + strand at 3110.248 kb on + strand at 3110.249 kb on - strand at 3110.249 kb on - strand at 3110.249 kb on - strand at 3110.249 kb on - strand at 3110.249 kb on - strand at 3110.249 kb on - strand at 3110.272 kb on - strand at 3110.272 kb on - strand at 3110.294 kb on - strand at 3110.381 kb on + strand, within yrbL at 3110.381 kb on + strand, within yrbL at 3110.408 kb on + strand, within yrbL at 3110.408 kb on + strand, within yrbL at 3110.409 kb on - strand, within yrbL at 3110.501 kb on + strand, within yrbL at 3110.618 kb on - strand, within yrbL at 3110.687 kb on - strand, within yrbL at 3110.687 kb on - strand, within yrbL at 3110.687 kb on - strand, within yrbL at 3110.729 kb on - strand, within yrbL at 3110.737 kb on - strand, within yrbL at 3110.780 kb on - strand, within yrbL at 3110.785 kb on - strand, within yrbL at 3110.785 kb on - strand, within yrbL at 3110.785 kb on - strand, within yrbL at 3110.785 kb on - strand, within yrbL at 3110.785 kb on - strand, within yrbL at 3110.804 kb on + strand, within yrbL at 3110.913 kb on + strand at 3110.914 kb on - strand at 3110.940 kb on + strand at 3110.971 kb on - strand at 3111.000 kb on + strand at 3111.119 kb on + strand at 3111.119 kb on + strand at 3111.120 kb on - strand at 3111.161 kb on + strand at 3111.162 kb on - strand at 3111.162 kb on - strand at 3111.299 kb on + strand, within npr at 3111.299 kb on + strand, within npr at 3111.299 kb on + strand, within npr at 3111.300 kb on - strand, within npr at 3111.300 kb on - strand, within npr at 3111.300 kb on - strand, within npr at 3111.303 kb on + strand, within npr at 3111.303 kb on + strand, within npr at 3111.304 kb on - strand, within npr at 3111.333 kb on + strand, within npr at 3111.333 kb on + strand, within npr at 3111.471 kb on + strand at 3111.471 kb on + strand at 3111.494 kb on - strand, within rapZ at 3111.495 kb on + strand, within rapZ at 3111.495 kb on + strand, within rapZ at 3111.495 kb on + strand, within rapZ at 3111.496 kb on - strand, within rapZ at 3111.543 kb on + strand, within rapZ at 3111.543 kb on + strand, within rapZ at 3111.544 kb on - strand, within rapZ at 3111.547 kb on + strand, within rapZ at 3111.548 kb on - strand, within rapZ at 3111.548 kb on - strand, within rapZ at 3111.548 kb on - strand, within rapZ at 3111.568 kb on + strand, within rapZ at 3111.568 kb on + strand, within rapZ at 3111.568 kb on + strand, within rapZ at 3111.569 kb on - strand, within rapZ at 3111.569 kb on - strand, within rapZ at 3111.569 kb on - strand, within rapZ at 3111.573 kb on - strand, within rapZ at 3111.573 kb on - strand, within rapZ at 3111.578 kb on + strand, within rapZ at 3111.578 kb on + strand, within rapZ at 3111.579 kb on - strand, within rapZ at 3111.579 kb on - strand, within rapZ at 3111.579 kb on - strand, within rapZ at 3111.579 kb on - strand, within rapZ at 3111.579 kb on - strand, within rapZ at 3111.588 kb on + strand, within rapZ at 3111.588 kb on + strand, within rapZ at 3111.603 kb on + strand, within rapZ at 3111.604 kb on - strand, within rapZ at 3111.608 kb on - strand, within rapZ at 3111.617 kb on + strand, within rapZ at 3111.727 kb on - strand, within rapZ at 3111.727 kb on - strand, within rapZ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,109,321 + elbB NIAGMN_15665 0.62 +0.7 3,109,321 + elbB NIAGMN_15665 0.62 -0.4 3,109,321 + elbB NIAGMN_15665 0.62 +3.0 3,109,321 + elbB NIAGMN_15665 0.62 +0.3 3,109,321 + elbB NIAGMN_15665 0.62 -1.6 3,109,322 - elbB NIAGMN_15665 0.62 +0.0 3,109,378 + elbB NIAGMN_15665 0.70 -0.4 3,109,468 + elbB NIAGMN_15665 0.84 +0.2 3,109,469 - elbB NIAGMN_15665 0.84 -1.8 3,109,490 + elbB NIAGMN_15665 0.88 +0.2 3,109,490 + elbB NIAGMN_15665 0.88 +0.4 3,109,491 - elbB NIAGMN_15665 0.88 -2.8 3,109,491 - elbB NIAGMN_15665 0.88 +1.5 3,109,491 - elbB NIAGMN_15665 0.88 +2.0 3,109,491 - elbB NIAGMN_15665 0.88 -0.3 3,109,492 + elbB NIAGMN_15665 0.88 -2.1 3,109,492 + elbB NIAGMN_15665 0.88 -1.0 3,109,492 + elbB NIAGMN_15665 0.88 -1.1 3,109,492 + elbB NIAGMN_15665 0.88 +1.0 3,109,492 + elbB NIAGMN_15665 0.88 +1.2 3,109,492 + elbB NIAGMN_15665 0.88 -1.4 3,109,492 + elbB NIAGMN_15665 0.88 +0.6 3,109,492 + elbB NIAGMN_15665 0.88 +0.3 3,109,492 + elbB NIAGMN_15665 0.88 -2.9 3,109,492 + elbB NIAGMN_15665 0.88 +0.6 3,109,492 + elbB NIAGMN_15665 0.88 +0.4 3,109,493 - elbB NIAGMN_15665 0.88 -2.0 3,109,493 - elbB NIAGMN_15665 0.88 +2.1 3,109,493 - elbB NIAGMN_15665 0.88 -2.9 3,109,493 - elbB NIAGMN_15665 0.88 -1.4 3,109,493 - elbB NIAGMN_15665 0.88 -0.6 3,109,493 - elbB NIAGMN_15665 0.88 -0.4 3,109,493 - elbB NIAGMN_15665 0.88 -2.8 3,109,493 - elbB NIAGMN_15665 0.88 +3.0 3,109,493 - elbB NIAGMN_15665 0.88 +0.7 3,109,493 - elbB NIAGMN_15665 0.88 +1.4 3,109,493 - elbB NIAGMN_15665 0.88 -2.8 3,109,534 + +1.1 3,109,534 + +1.3 3,109,534 + -2.4 3,109,534 + -0.4 3,109,535 - -0.7 3,109,535 - -1.6 3,109,593 + -2.0 3,109,593 + -3.9 3,109,593 + +0.8 3,109,668 + mtgA NIAGMN_15670 0.14 +0.5 3,109,734 + mtgA NIAGMN_15670 0.23 +0.8 3,109,744 + mtgA NIAGMN_15670 0.24 -0.0 3,109,745 - mtgA NIAGMN_15670 0.24 +0.6 3,110,009 + mtgA NIAGMN_15670 0.61 -1.1 3,110,009 + mtgA NIAGMN_15670 0.61 +2.2 3,110,107 + mtgA NIAGMN_15670 0.74 +0.0 3,110,108 - mtgA NIAGMN_15670 0.74 +1.1 3,110,108 - mtgA NIAGMN_15670 0.74 +0.8 3,110,133 + mtgA NIAGMN_15670 0.78 -2.4 3,110,134 - mtgA NIAGMN_15670 0.78 -0.4 3,110,134 - mtgA NIAGMN_15670 0.78 +0.7 3,110,154 - mtgA NIAGMN_15670 0.81 -1.2 3,110,165 + mtgA NIAGMN_15670 0.82 -2.5 3,110,166 - mtgA NIAGMN_15670 0.82 -0.4 3,110,166 - mtgA NIAGMN_15670 0.82 -3.5 3,110,166 - mtgA NIAGMN_15670 0.82 -0.3 3,110,168 + mtgA NIAGMN_15670 0.82 -0.6 3,110,168 + mtgA NIAGMN_15670 0.82 -1.8 3,110,168 + mtgA NIAGMN_15670 0.82 +1.1 3,110,168 + mtgA NIAGMN_15670 0.82 +0.9 3,110,169 - mtgA NIAGMN_15670 0.83 +0.8 3,110,169 - mtgA NIAGMN_15670 0.83 -1.5 3,110,169 - mtgA NIAGMN_15670 0.83 +2.4 3,110,169 - mtgA NIAGMN_15670 0.83 -2.1 3,110,169 - mtgA NIAGMN_15670 0.83 -2.9 3,110,173 - mtgA NIAGMN_15670 0.83 -0.9 3,110,173 - mtgA NIAGMN_15670 0.83 +0.4 3,110,173 - mtgA NIAGMN_15670 0.83 +1.1 3,110,248 + +0.7 3,110,248 + -1.2 3,110,248 + -0.1 3,110,249 - -1.3 3,110,249 - +0.3 3,110,249 - +0.4 3,110,249 - +1.6 3,110,249 - +2.0 3,110,249 - +0.0 3,110,272 - +1.2 3,110,272 - +0.0 3,110,294 - +0.4 3,110,381 + yrbL NIAGMN_15675 0.14 +2.6 3,110,381 + yrbL NIAGMN_15675 0.14 +0.0 3,110,408 + yrbL NIAGMN_15675 0.18 -0.1 3,110,408 + yrbL NIAGMN_15675 0.18 +3.1 3,110,409 - yrbL NIAGMN_15675 0.18 +3.1 3,110,501 + yrbL NIAGMN_15675 0.33 -3.0 3,110,618 - yrbL NIAGMN_15675 0.52 -1.2 3,110,687 - yrbL NIAGMN_15675 0.62 -2.2 3,110,687 - yrbL NIAGMN_15675 0.62 +0.4 3,110,687 - yrbL NIAGMN_15675 0.62 -2.8 3,110,729 - yrbL NIAGMN_15675 0.69 -1.6 3,110,737 - yrbL NIAGMN_15675 0.70 -3.6 3,110,780 - yrbL NIAGMN_15675 0.77 -1.7 3,110,785 - yrbL NIAGMN_15675 0.78 -2.8 3,110,785 - yrbL NIAGMN_15675 0.78 -0.9 3,110,785 - yrbL NIAGMN_15675 0.78 +0.4 3,110,785 - yrbL NIAGMN_15675 0.78 +2.0 3,110,785 - yrbL NIAGMN_15675 0.78 +0.0 3,110,804 + yrbL NIAGMN_15675 0.81 +1.6 3,110,913 + -0.6 3,110,914 - +1.2 3,110,940 + -0.5 3,110,971 - -2.8 3,111,000 + +0.4 3,111,119 + +1.4 3,111,119 + +0.4 3,111,120 - +2.1 3,111,161 + +3.6 3,111,162 - -2.5 3,111,162 - +2.8 3,111,299 + npr NIAGMN_15680 0.59 -1.6 3,111,299 + npr NIAGMN_15680 0.59 +1.5 3,111,299 + npr NIAGMN_15680 0.59 +1.2 3,111,300 - npr NIAGMN_15680 0.59 -2.9 3,111,300 - npr NIAGMN_15680 0.59 -0.1 3,111,300 - npr NIAGMN_15680 0.59 -3.3 3,111,303 + npr NIAGMN_15680 0.60 -0.3 3,111,303 + npr NIAGMN_15680 0.60 +1.7 3,111,304 - npr NIAGMN_15680 0.61 +1.3 3,111,333 + npr NIAGMN_15680 0.71 +3.5 3,111,333 + npr NIAGMN_15680 0.71 +2.4 3,111,471 + -1.8 3,111,471 + -0.3 3,111,494 - rapZ NIAGMN_15685 0.10 -1.7 3,111,495 + rapZ NIAGMN_15685 0.10 -4.0 3,111,495 + rapZ NIAGMN_15685 0.10 -0.9 3,111,495 + rapZ NIAGMN_15685 0.10 +0.0 3,111,496 - rapZ NIAGMN_15685 0.10 +1.5 3,111,543 + rapZ NIAGMN_15685 0.16 -0.2 3,111,543 + rapZ NIAGMN_15685 0.16 -0.2 3,111,544 - rapZ NIAGMN_15685 0.16 -0.7 3,111,547 + rapZ NIAGMN_15685 0.16 +1.0 3,111,548 - rapZ NIAGMN_15685 0.16 -0.4 3,111,548 - rapZ NIAGMN_15685 0.16 -1.2 3,111,548 - rapZ NIAGMN_15685 0.16 +2.2 3,111,568 + rapZ NIAGMN_15685 0.19 -1.7 3,111,568 + rapZ NIAGMN_15685 0.19 -0.3 3,111,568 + rapZ NIAGMN_15685 0.19 -1.9 3,111,569 - rapZ NIAGMN_15685 0.19 -0.2 3,111,569 - rapZ NIAGMN_15685 0.19 -1.1 3,111,569 - rapZ NIAGMN_15685 0.19 -2.9 3,111,573 - rapZ NIAGMN_15685 0.19 -0.2 3,111,573 - rapZ NIAGMN_15685 0.19 -1.4 3,111,578 + rapZ NIAGMN_15685 0.20 -0.3 3,111,578 + rapZ NIAGMN_15685 0.20 +0.0 3,111,579 - rapZ NIAGMN_15685 0.20 -1.6 3,111,579 - rapZ NIAGMN_15685 0.20 -0.2 3,111,579 - rapZ NIAGMN_15685 0.20 -2.0 3,111,579 - rapZ NIAGMN_15685 0.20 +0.4 3,111,579 - rapZ NIAGMN_15685 0.20 -0.3 3,111,588 + rapZ NIAGMN_15685 0.21 +1.0 3,111,588 + rapZ NIAGMN_15685 0.21 -1.4 3,111,603 + rapZ NIAGMN_15685 0.23 +0.2 3,111,604 - rapZ NIAGMN_15685 0.23 +0.3 3,111,608 - rapZ NIAGMN_15685 0.24 -1.8 3,111,617 + rapZ NIAGMN_15685 0.25 -1.3 3,111,727 - rapZ NIAGMN_15685 0.37 -0.0 3,111,727 - rapZ NIAGMN_15685 0.37 -1.6
Or see this region's nucleotide sequence