Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt 16S_rrna and Ile_trna are separated by 68 nucleotides Ile_trna and Ala_trna are separated by 42 nucleotides Ala_trna and 23S_rrna are separated by 183 nucleotides 23S_rrna and 5S_rrna are separated by 94 nucleotides 5S_rrna and Thr_trna are separated by 14 nucleotides Thr_trna and 5S_rrna are separated by 39 nucleotides 5S_rrna and yhdZ are separated by 230 nucleotides
NIAGMN_15340: 16S_rrna - 16S ribosomal RNA, at 3,037,954 to 3,039,495
_rrna
NIAGMN_15345: Ile_trna - tRNA-Ile(gat), at 3,039,564 to 3,039,640
_trna
NIAGMN_15350: Ala_trna - tRNA-Ala(tgc), at 3,039,683 to 3,039,758
_trna
NIAGMN_15360: 23S_rrna - 23S ribosomal RNA, at 3,039,942 to 3,042,844
_rrna
NIAGMN_15365: 5S_rrna - 5S ribosomal RNA, at 3,042,939 to 3,043,054
_rrna
NIAGMN_15370: Thr_trna - tRNA-Thr(ggt), at 3,043,069 to 3,043,144
_trna
NIAGMN_15375: 5S_rrna - 5S ribosomal RNA, at 3,043,184 to 3,043,299
_rrna
NIAGMN_15380: yhdZ - Uncharacterized amino-acid ABC transporter ATP-binding protein YhdZ, at 3,043,530 to 3,044,288
yhdZ
Position (kb)
3039
3040
3041
3042
3043 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3043.104 kb on + strand, within Thr_trna at 3043.104 kb on + strand, within Thr_trna at 3043.150 kb on + strand at 3043.170 kb on + strand at 3043.284 kb on + strand, within 5S_rrna at 3043.507 kb on - strand at 3043.507 kb on - strand at 3043.521 kb on - strand at 3043.521 kb on - strand at 3043.521 kb on - strand at 3043.532 kb on - strand at 3043.540 kb on - strand at 3043.549 kb on - strand at 3043.615 kb on - strand, within yhdZ at 3043.785 kb on - strand, within yhdZ at 3043.785 kb on - strand, within yhdZ at 3043.800 kb on - strand, within yhdZ at 3043.800 kb on - strand, within yhdZ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,043,104 + Thr_trna NIAGMN_15370 0.46 +0.1 3,043,104 + Thr_trna NIAGMN_15370 0.46 +1.4 3,043,150 + -0.3 3,043,170 + -0.6 3,043,284 + 5S_rrna NIAGMN_15375 0.86 -2.1 3,043,507 - +0.6 3,043,507 - +1.2 3,043,521 - -1.7 3,043,521 - +0.0 3,043,521 - +0.2 3,043,532 - -0.4 3,043,540 - -1.7 3,043,549 - +1.1 3,043,615 - yhdZ NIAGMN_15380 0.11 +0.1 3,043,785 - yhdZ NIAGMN_15380 0.34 -0.1 3,043,785 - yhdZ NIAGMN_15380 0.34 +0.3 3,043,800 - yhdZ NIAGMN_15380 0.36 +3.0 3,043,800 - yhdZ NIAGMN_15380 0.36 +0.6
Or see this region's nucleotide sequence