Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpD and glpE are separated by 189 nucleotides glpE and glpG are separated by 44 nucleotides glpG and glpR are separated by 16 nucleotides glpR and rtcR overlap by 19 nucleotides
NIAGMN_14695: glpD - glycerol-3-phosphate dehydrogenase, at 2,925,263 to 2,926,768
glpD
NIAGMN_14700: glpE - thiosulfate sulfurtransferase GlpE, at 2,926,958 to 2,927,284
glpE
NIAGMN_14705: glpG - rhomboid family intramembrane serine protease GlpG, at 2,927,329 to 2,928,159
glpG
NIAGMN_14710: glpR - DeoR/GlpR family transcriptional regulator, at 2,928,176 to 2,928,934
glpR
NIAGMN_14715: rtcR - DNA-binding transcriptional regulator RtcR, at 2,928,916 to 2,930,514
rtcR
Position (kb)
2927
2928
2929 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2926.364 kb on - strand, within glpD at 2926.447 kb on - strand, within glpD at 2926.819 kb on + strand at 2926.820 kb on - strand at 2926.871 kb on + strand at 2926.871 kb on + strand at 2926.872 kb on - strand at 2926.875 kb on + strand at 2926.875 kb on + strand at 2926.876 kb on - strand at 2926.876 kb on - strand at 2926.876 kb on - strand at 2926.877 kb on + strand at 2926.877 kb on + strand at 2926.878 kb on - strand at 2926.892 kb on + strand at 2926.892 kb on + strand at 2926.893 kb on - strand at 2926.893 kb on - strand at 2926.893 kb on - strand at 2926.893 kb on - strand at 2926.893 kb on - strand at 2926.942 kb on - strand at 2926.975 kb on + strand at 2926.975 kb on + strand at 2926.975 kb on + strand at 2926.975 kb on + strand at 2926.976 kb on - strand at 2926.978 kb on + strand at 2926.979 kb on - strand at 2926.979 kb on - strand at 2926.979 kb on - strand at 2926.979 kb on - strand at 2927.033 kb on - strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.116 kb on + strand, within glpE at 2927.117 kb on - strand, within glpE at 2927.117 kb on - strand, within glpE at 2927.154 kb on - strand, within glpE at 2927.154 kb on - strand, within glpE at 2927.164 kb on + strand, within glpE at 2927.164 kb on + strand, within glpE at 2927.165 kb on - strand, within glpE at 2927.186 kb on + strand, within glpE at 2927.187 kb on - strand, within glpE at 2927.204 kb on + strand, within glpE at 2927.204 kb on + strand, within glpE at 2927.216 kb on + strand, within glpE at 2927.216 kb on + strand, within glpE at 2927.216 kb on + strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.217 kb on - strand, within glpE at 2927.218 kb on + strand, within glpE at 2927.218 kb on + strand, within glpE at 2927.219 kb on - strand, within glpE at 2927.219 kb on - strand, within glpE at 2927.219 kb on - strand, within glpE at 2927.290 kb on + strand at 2927.290 kb on + strand at 2927.291 kb on - strand at 2927.291 kb on - strand at 2927.292 kb on + strand at 2927.292 kb on + strand at 2927.292 kb on + strand at 2927.292 kb on + strand at 2927.292 kb on + strand at 2927.292 kb on + strand at 2927.293 kb on - strand at 2927.340 kb on + strand at 2927.340 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.383 kb on + strand at 2927.384 kb on - strand at 2927.384 kb on - strand at 2927.404 kb on - strand at 2927.424 kb on + strand, within glpG at 2927.424 kb on + strand, within glpG at 2927.424 kb on + strand, within glpG at 2927.425 kb on - strand, within glpG at 2927.425 kb on - strand, within glpG at 2927.652 kb on + strand, within glpG at 2927.652 kb on + strand, within glpG at 2927.690 kb on + strand, within glpG at 2927.740 kb on + strand, within glpG at 2927.756 kb on + strand, within glpG at 2927.756 kb on + strand, within glpG at 2927.778 kb on + strand, within glpG at 2927.779 kb on - strand, within glpG at 2927.788 kb on - strand, within glpG at 2927.806 kb on + strand, within glpG at 2927.849 kb on + strand, within glpG at 2927.849 kb on + strand, within glpG at 2927.850 kb on - strand, within glpG at 2927.850 kb on - strand, within glpG at 2927.860 kb on - strand, within glpG at 2927.887 kb on + strand, within glpG at 2927.941 kb on + strand, within glpG at 2927.941 kb on + strand, within glpG at 2927.942 kb on - strand, within glpG at 2927.942 kb on - strand, within glpG at 2927.942 kb on - strand, within glpG at 2927.942 kb on - strand, within glpG at 2927.956 kb on + strand, within glpG at 2927.966 kb on + strand, within glpG at 2927.967 kb on - strand, within glpG at 2927.999 kb on - strand, within glpG at 2928.014 kb on + strand, within glpG at 2928.015 kb on - strand, within glpG at 2928.018 kb on + strand, within glpG at 2928.157 kb on + strand at 2928.203 kb on - strand at 2928.203 kb on - strand at 2928.217 kb on + strand at 2928.230 kb on + strand at 2928.230 kb on + strand at 2928.230 kb on + strand at 2928.230 kb on + strand at 2928.230 kb on + strand at 2928.231 kb on - strand at 2928.231 kb on - strand at 2928.231 kb on - strand at 2928.231 kb on - strand at 2928.231 kb on - strand at 2928.238 kb on + strand at 2928.280 kb on + strand, within glpR at 2928.554 kb on - strand, within glpR at 2928.554 kb on - strand, within glpR at 2928.606 kb on + strand, within glpR at 2928.607 kb on - strand, within glpR at 2928.652 kb on + strand, within glpR at 2928.781 kb on + strand, within glpR at 2928.781 kb on + strand, within glpR at 2928.782 kb on - strand, within glpR at 2928.829 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.849 kb on + strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.850 kb on - strand, within glpR at 2928.882 kb on + strand at 2928.882 kb on + strand at 2928.913 kb on + strand at 2928.914 kb on - strand at 2928.974 kb on + strand at 2928.975 kb on - strand at 2929.101 kb on + strand, within rtcR
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,926,364 - glpD NIAGMN_14695 0.73 -2.9 2,926,447 - glpD NIAGMN_14695 0.79 -1.2 2,926,819 + -0.5 2,926,820 - -3.7 2,926,871 + -2.9 2,926,871 + +0.2 2,926,872 - +0.6 2,926,875 + -0.8 2,926,875 + +1.2 2,926,876 - -0.8 2,926,876 - +2.0 2,926,876 - +0.2 2,926,877 + +2.3 2,926,877 + +1.6 2,926,878 - -0.4 2,926,892 + +0.6 2,926,892 + -3.0 2,926,893 - +1.3 2,926,893 - -0.2 2,926,893 - -1.3 2,926,893 - +0.0 2,926,893 - -0.7 2,926,942 - +0.1 2,926,975 + -0.5 2,926,975 + +0.3 2,926,975 + -0.3 2,926,975 + -0.2 2,926,976 - -2.8 2,926,978 + -3.4 2,926,979 - -0.0 2,926,979 - +1.6 2,926,979 - -1.2 2,926,979 - -1.4 2,927,033 - glpE NIAGMN_14700 0.23 -1.9 2,927,116 + glpE NIAGMN_14700 0.48 -0.8 2,927,116 + glpE NIAGMN_14700 0.48 +0.9 2,927,116 + glpE NIAGMN_14700 0.48 +0.0 2,927,116 + glpE NIAGMN_14700 0.48 -3.4 2,927,116 + glpE NIAGMN_14700 0.48 +0.4 2,927,116 + glpE NIAGMN_14700 0.48 -1.6 2,927,116 + glpE NIAGMN_14700 0.48 +1.9 2,927,117 - glpE NIAGMN_14700 0.49 -2.0 2,927,117 - glpE NIAGMN_14700 0.49 -2.7 2,927,154 - glpE NIAGMN_14700 0.60 -2.4 2,927,154 - glpE NIAGMN_14700 0.60 -1.0 2,927,164 + glpE NIAGMN_14700 0.63 -0.4 2,927,164 + glpE NIAGMN_14700 0.63 -0.0 2,927,165 - glpE NIAGMN_14700 0.63 -1.3 2,927,186 + glpE NIAGMN_14700 0.70 -1.1 2,927,187 - glpE NIAGMN_14700 0.70 -1.2 2,927,204 + glpE NIAGMN_14700 0.75 -1.7 2,927,204 + glpE NIAGMN_14700 0.75 +0.8 2,927,216 + glpE NIAGMN_14700 0.79 +0.0 2,927,216 + glpE NIAGMN_14700 0.79 +0.6 2,927,216 + glpE NIAGMN_14700 0.79 +0.6 2,927,217 - glpE NIAGMN_14700 0.79 -1.2 2,927,217 - glpE NIAGMN_14700 0.79 +0.5 2,927,217 - glpE NIAGMN_14700 0.79 -0.9 2,927,217 - glpE NIAGMN_14700 0.79 -2.4 2,927,217 - glpE NIAGMN_14700 0.79 +1.1 2,927,217 - glpE NIAGMN_14700 0.79 -2.3 2,927,217 - glpE NIAGMN_14700 0.79 +1.5 2,927,217 - glpE NIAGMN_14700 0.79 -2.0 2,927,218 + glpE NIAGMN_14700 0.80 -1.3 2,927,218 + glpE NIAGMN_14700 0.80 +1.2 2,927,219 - glpE NIAGMN_14700 0.80 -0.5 2,927,219 - glpE NIAGMN_14700 0.80 -3.6 2,927,219 - glpE NIAGMN_14700 0.80 +1.2 2,927,290 + +0.2 2,927,290 + -0.7 2,927,291 - -1.6 2,927,291 - +0.2 2,927,292 + +0.3 2,927,292 + -0.9 2,927,292 + -0.9 2,927,292 + +1.1 2,927,292 + -0.0 2,927,292 + +0.4 2,927,293 - +0.3 2,927,340 + -1.6 2,927,340 + -0.1 2,927,383 + -0.6 2,927,383 + +1.4 2,927,383 + +1.0 2,927,383 + -3.4 2,927,383 + -0.0 2,927,383 + +0.7 2,927,383 + -4.2 2,927,383 + -1.9 2,927,384 - +2.5 2,927,384 - +0.4 2,927,404 - +0.1 2,927,424 + glpG NIAGMN_14705 0.11 -1.0 2,927,424 + glpG NIAGMN_14705 0.11 -0.2 2,927,424 + glpG NIAGMN_14705 0.11 -1.0 2,927,425 - glpG NIAGMN_14705 0.12 -0.2 2,927,425 - glpG NIAGMN_14705 0.12 +1.3 2,927,652 + glpG NIAGMN_14705 0.39 +1.3 2,927,652 + glpG NIAGMN_14705 0.39 -0.5 2,927,690 + glpG NIAGMN_14705 0.43 -3.2 2,927,740 + glpG NIAGMN_14705 0.49 -0.4 2,927,756 + glpG NIAGMN_14705 0.51 -0.0 2,927,756 + glpG NIAGMN_14705 0.51 -1.1 2,927,778 + glpG NIAGMN_14705 0.54 +0.6 2,927,779 - glpG NIAGMN_14705 0.54 -1.6 2,927,788 - glpG NIAGMN_14705 0.55 -2.9 2,927,806 + glpG NIAGMN_14705 0.57 -1.6 2,927,849 + glpG NIAGMN_14705 0.63 -2.9 2,927,849 + glpG NIAGMN_14705 0.63 +2.4 2,927,850 - glpG NIAGMN_14705 0.63 -1.7 2,927,850 - glpG NIAGMN_14705 0.63 -2.0 2,927,860 - glpG NIAGMN_14705 0.64 -1.3 2,927,887 + glpG NIAGMN_14705 0.67 -1.9 2,927,941 + glpG NIAGMN_14705 0.74 +0.0 2,927,941 + glpG NIAGMN_14705 0.74 -0.1 2,927,942 - glpG NIAGMN_14705 0.74 -0.1 2,927,942 - glpG NIAGMN_14705 0.74 +1.6 2,927,942 - glpG NIAGMN_14705 0.74 -2.7 2,927,942 - glpG NIAGMN_14705 0.74 -1.7 2,927,956 + glpG NIAGMN_14705 0.75 +0.6 2,927,966 + glpG NIAGMN_14705 0.77 -0.1 2,927,967 - glpG NIAGMN_14705 0.77 +1.0 2,927,999 - glpG NIAGMN_14705 0.81 -0.6 2,928,014 + glpG NIAGMN_14705 0.82 +0.5 2,928,015 - glpG NIAGMN_14705 0.83 -0.5 2,928,018 + glpG NIAGMN_14705 0.83 +0.7 2,928,157 + +2.4 2,928,203 - -0.8 2,928,203 - -0.7 2,928,217 + -0.6 2,928,230 + -3.5 2,928,230 + +0.9 2,928,230 + -0.7 2,928,230 + +0.1 2,928,230 + -2.2 2,928,231 - -1.4 2,928,231 - +1.1 2,928,231 - +0.5 2,928,231 - -2.5 2,928,231 - +0.6 2,928,238 + -0.1 2,928,280 + glpR NIAGMN_14710 0.14 -1.8 2,928,554 - glpR NIAGMN_14710 0.50 -2.0 2,928,554 - glpR NIAGMN_14710 0.50 -1.1 2,928,606 + glpR NIAGMN_14710 0.57 +0.1 2,928,607 - glpR NIAGMN_14710 0.57 -1.7 2,928,652 + glpR NIAGMN_14710 0.63 -0.3 2,928,781 + glpR NIAGMN_14710 0.80 +0.4 2,928,781 + glpR NIAGMN_14710 0.80 +0.1 2,928,782 - glpR NIAGMN_14710 0.80 -2.1 2,928,829 + glpR NIAGMN_14710 0.86 -0.3 2,928,849 + glpR NIAGMN_14710 0.89 -1.7 2,928,849 + glpR NIAGMN_14710 0.89 +1.2 2,928,849 + glpR NIAGMN_14710 0.89 +0.8 2,928,849 + glpR NIAGMN_14710 0.89 -3.3 2,928,849 + glpR NIAGMN_14710 0.89 -1.2 2,928,849 + glpR NIAGMN_14710 0.89 -2.9 2,928,849 + glpR NIAGMN_14710 0.89 -4.3 2,928,849 + glpR NIAGMN_14710 0.89 -1.4 2,928,850 - glpR NIAGMN_14710 0.89 -1.5 2,928,850 - glpR NIAGMN_14710 0.89 -0.7 2,928,850 - glpR NIAGMN_14710 0.89 -0.4 2,928,850 - glpR NIAGMN_14710 0.89 +0.3 2,928,850 - glpR NIAGMN_14710 0.89 +0.4 2,928,850 - glpR NIAGMN_14710 0.89 -1.3 2,928,850 - glpR NIAGMN_14710 0.89 +0.2 2,928,882 + +0.0 2,928,882 + -2.5 2,928,913 + +0.2 2,928,914 - -2.2 2,928,974 + -2.8 2,928,975 - +0.2 2,929,101 + rtcR NIAGMN_14715 0.12 +0.5
Or see this region's nucleotide sequence