Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ghrB and yiaD are separated by 103 nucleotides yiaD and bisC are separated by 152 nucleotides
NIAGMN_13780: ghrB - glyoxylate/hydroxypyruvate reductase GhrB, at 2,735,874 to 2,736,848
ghrB
NIAGMN_13785: yiaD - OmpA family lipoprotein, at 2,736,952 to 2,737,611
yiaD
NIAGMN_13790: bisC - biotin sulfoxide reductase, at 2,737,764 to 2,740,097
bisC
Position (kb)
2736
2737
2738 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2735.993 kb on + strand, within ghrB at 2735.993 kb on + strand, within ghrB at 2736.219 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.255 kb on + strand, within ghrB at 2736.256 kb on - strand, within ghrB at 2736.256 kb on - strand, within ghrB at 2736.263 kb on + strand, within ghrB at 2736.263 kb on + strand, within ghrB at 2736.264 kb on - strand, within ghrB at 2736.264 kb on - strand, within ghrB at 2736.308 kb on + strand, within ghrB at 2736.308 kb on + strand, within ghrB at 2736.309 kb on - strand, within ghrB at 2736.329 kb on - strand, within ghrB at 2736.329 kb on - strand, within ghrB at 2736.413 kb on + strand, within ghrB at 2736.414 kb on - strand, within ghrB at 2736.414 kb on - strand, within ghrB at 2736.434 kb on - strand, within ghrB at 2736.448 kb on - strand, within ghrB at 2736.619 kb on + strand, within ghrB at 2736.702 kb on + strand, within ghrB at 2736.702 kb on + strand, within ghrB at 2736.804 kb on + strand at 2736.804 kb on + strand at 2736.817 kb on - strand at 2736.826 kb on + strand at 2736.826 kb on + strand at 2736.826 kb on + strand at 2736.826 kb on + strand at 2736.827 kb on - strand at 2736.868 kb on + strand at 2736.868 kb on + strand at 2736.883 kb on - strand at 2736.893 kb on - strand at 2736.893 kb on - strand at 2736.954 kb on - strand at 2736.954 kb on - strand at 2736.954 kb on - strand at 2736.964 kb on + strand at 2736.969 kb on + strand at 2736.969 kb on + strand at 2736.970 kb on - strand at 2736.976 kb on + strand at 2736.996 kb on + strand at 2736.997 kb on - strand at 2736.997 kb on - strand at 2736.997 kb on - strand at 2737.051 kb on - strand, within yiaD at 2737.158 kb on + strand, within yiaD at 2737.158 kb on + strand, within yiaD at 2737.158 kb on + strand, within yiaD at 2737.159 kb on - strand, within yiaD at 2737.159 kb on - strand, within yiaD at 2737.160 kb on + strand, within yiaD at 2737.160 kb on + strand, within yiaD at 2737.161 kb on - strand, within yiaD at 2737.284 kb on + strand, within yiaD at 2737.294 kb on - strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.373 kb on + strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.374 kb on - strand, within yiaD at 2737.513 kb on - strand, within yiaD at 2737.513 kb on - strand, within yiaD at 2737.535 kb on + strand, within yiaD at 2737.542 kb on + strand, within yiaD at 2737.543 kb on - strand, within yiaD at 2737.543 kb on - strand, within yiaD at 2737.543 kb on - strand, within yiaD at 2737.590 kb on + strand at 2737.591 kb on - strand at 2737.591 kb on - strand at 2737.595 kb on + strand at 2737.595 kb on + strand at 2737.595 kb on + strand at 2737.622 kb on + strand at 2737.623 kb on - strand at 2737.623 kb on - strand at 2737.634 kb on + strand at 2737.634 kb on + strand at 2737.635 kb on - strand at 2737.666 kb on + strand at 2737.666 kb on + strand at 2737.666 kb on + strand at 2737.688 kb on + strand at 2737.688 kb on + strand at 2737.689 kb on - strand at 2737.689 kb on - strand at 2737.689 kb on - strand at 2737.718 kb on + strand at 2737.726 kb on - strand at 2737.736 kb on + strand at 2737.736 kb on + strand at 2737.736 kb on + strand at 2737.736 kb on + strand at 2737.737 kb on - strand at 2737.737 kb on - strand at 2737.737 kb on - strand at 2737.737 kb on - strand at 2737.742 kb on + strand at 2737.742 kb on + strand at 2737.742 kb on + strand at 2737.743 kb on - strand at 2737.743 kb on - strand at 2737.743 kb on - strand at 2737.851 kb on - strand at 2737.925 kb on + strand at 2737.934 kb on - strand at 2738.042 kb on + strand, within bisC at 2738.043 kb on - strand, within bisC at 2738.057 kb on + strand, within bisC at 2738.057 kb on + strand, within bisC at 2738.057 kb on + strand, within bisC at 2738.057 kb on + strand, within bisC at 2738.057 kb on + strand, within bisC at 2738.058 kb on - strand, within bisC at 2738.076 kb on + strand, within bisC at 2738.076 kb on + strand, within bisC at 2738.076 kb on + strand, within bisC at 2738.077 kb on - strand, within bisC at 2738.136 kb on - strand, within bisC at 2738.136 kb on - strand, within bisC at 2738.149 kb on + strand, within bisC at 2738.150 kb on - strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.152 kb on + strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.153 kb on - strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.160 kb on + strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.161 kb on - strand, within bisC at 2738.162 kb on + strand, within bisC at 2738.162 kb on + strand, within bisC at 2738.163 kb on - strand, within bisC at 2738.181 kb on + strand, within bisC at 2738.181 kb on + strand, within bisC at 2738.181 kb on + strand, within bisC at 2738.181 kb on + strand, within bisC at 2738.183 kb on + strand, within bisC at 2738.203 kb on - strand, within bisC at 2738.235 kb on + strand, within bisC at 2738.235 kb on + strand, within bisC at 2738.235 kb on + strand, within bisC at 2738.236 kb on - strand, within bisC at 2738.236 kb on - strand, within bisC at 2738.236 kb on - strand, within bisC at 2738.249 kb on + strand, within bisC at 2738.259 kb on + strand, within bisC at 2738.298 kb on - strand, within bisC at 2738.298 kb on - strand, within bisC at 2738.337 kb on - strand, within bisC at 2738.382 kb on + strand, within bisC at 2738.529 kb on - strand, within bisC at 2738.587 kb on - strand, within bisC at 2738.604 kb on + strand, within bisC at 2738.604 kb on + strand, within bisC at 2738.605 kb on - strand, within bisC at 2738.605 kb on - strand, within bisC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,735,993 + ghrB NIAGMN_13780 0.12 +0.7 2,735,993 + ghrB NIAGMN_13780 0.12 +1.8 2,736,219 + ghrB NIAGMN_13780 0.35 -0.1 2,736,255 + ghrB NIAGMN_13780 0.39 +1.0 2,736,255 + ghrB NIAGMN_13780 0.39 -0.1 2,736,255 + ghrB NIAGMN_13780 0.39 -1.4 2,736,255 + ghrB NIAGMN_13780 0.39 +1.5 2,736,255 + ghrB NIAGMN_13780 0.39 -0.5 2,736,255 + ghrB NIAGMN_13780 0.39 -0.6 2,736,255 + ghrB NIAGMN_13780 0.39 +1.0 2,736,255 + ghrB NIAGMN_13780 0.39 +2.8 2,736,256 - ghrB NIAGMN_13780 0.39 +0.4 2,736,256 - ghrB NIAGMN_13780 0.39 -1.4 2,736,263 + ghrB NIAGMN_13780 0.40 -2.4 2,736,263 + ghrB NIAGMN_13780 0.40 +0.5 2,736,264 - ghrB NIAGMN_13780 0.40 +1.0 2,736,264 - ghrB NIAGMN_13780 0.40 +0.2 2,736,308 + ghrB NIAGMN_13780 0.45 +0.2 2,736,308 + ghrB NIAGMN_13780 0.45 -1.6 2,736,309 - ghrB NIAGMN_13780 0.45 +0.2 2,736,329 - ghrB NIAGMN_13780 0.47 +0.6 2,736,329 - ghrB NIAGMN_13780 0.47 +2.2 2,736,413 + ghrB NIAGMN_13780 0.55 +0.3 2,736,414 - ghrB NIAGMN_13780 0.55 +1.0 2,736,414 - ghrB NIAGMN_13780 0.55 +0.6 2,736,434 - ghrB NIAGMN_13780 0.57 +0.3 2,736,448 - ghrB NIAGMN_13780 0.59 +1.1 2,736,619 + ghrB NIAGMN_13780 0.76 -0.2 2,736,702 + ghrB NIAGMN_13780 0.85 -0.7 2,736,702 + ghrB NIAGMN_13780 0.85 +1.1 2,736,804 + +1.0 2,736,804 + +0.6 2,736,817 - +2.2 2,736,826 + -0.3 2,736,826 + -0.7 2,736,826 + -2.5 2,736,826 + -1.7 2,736,827 - +1.6 2,736,868 + +2.8 2,736,868 + -1.8 2,736,883 - +2.6 2,736,893 - +0.1 2,736,893 - +0.6 2,736,954 - -2.2 2,736,954 - +1.2 2,736,954 - -1.4 2,736,964 + -0.2 2,736,969 + -2.3 2,736,969 + +0.6 2,736,970 - +1.2 2,736,976 + -3.2 2,736,996 + +1.0 2,736,997 - +0.0 2,736,997 - +0.3 2,736,997 - -0.5 2,737,051 - yiaD NIAGMN_13785 0.15 +0.7 2,737,158 + yiaD NIAGMN_13785 0.31 -0.9 2,737,158 + yiaD NIAGMN_13785 0.31 +2.9 2,737,158 + yiaD NIAGMN_13785 0.31 +1.6 2,737,159 - yiaD NIAGMN_13785 0.31 -0.3 2,737,159 - yiaD NIAGMN_13785 0.31 +3.4 2,737,160 + yiaD NIAGMN_13785 0.32 -1.3 2,737,160 + yiaD NIAGMN_13785 0.32 -0.8 2,737,161 - yiaD NIAGMN_13785 0.32 +0.5 2,737,284 + yiaD NIAGMN_13785 0.50 -3.6 2,737,294 - yiaD NIAGMN_13785 0.52 -0.7 2,737,373 + yiaD NIAGMN_13785 0.64 -2.2 2,737,373 + yiaD NIAGMN_13785 0.64 -2.0 2,737,373 + yiaD NIAGMN_13785 0.64 -0.3 2,737,373 + yiaD NIAGMN_13785 0.64 +0.7 2,737,373 + yiaD NIAGMN_13785 0.64 +2.3 2,737,373 + yiaD NIAGMN_13785 0.64 +0.4 2,737,373 + yiaD NIAGMN_13785 0.64 +1.9 2,737,373 + yiaD NIAGMN_13785 0.64 -0.7 2,737,373 + yiaD NIAGMN_13785 0.64 +1.2 2,737,374 - yiaD NIAGMN_13785 0.64 +0.5 2,737,374 - yiaD NIAGMN_13785 0.64 +0.0 2,737,374 - yiaD NIAGMN_13785 0.64 +0.3 2,737,374 - yiaD NIAGMN_13785 0.64 +0.2 2,737,374 - yiaD NIAGMN_13785 0.64 +0.4 2,737,374 - yiaD NIAGMN_13785 0.64 +0.4 2,737,374 - yiaD NIAGMN_13785 0.64 +0.8 2,737,374 - yiaD NIAGMN_13785 0.64 -0.9 2,737,374 - yiaD NIAGMN_13785 0.64 +2.7 2,737,374 - yiaD NIAGMN_13785 0.64 +0.2 2,737,374 - yiaD NIAGMN_13785 0.64 +0.4 2,737,374 - yiaD NIAGMN_13785 0.64 -2.6 2,737,513 - yiaD NIAGMN_13785 0.85 +0.2 2,737,513 - yiaD NIAGMN_13785 0.85 -1.4 2,737,535 + yiaD NIAGMN_13785 0.88 +0.5 2,737,542 + yiaD NIAGMN_13785 0.89 -0.0 2,737,543 - yiaD NIAGMN_13785 0.90 -2.2 2,737,543 - yiaD NIAGMN_13785 0.90 +0.0 2,737,543 - yiaD NIAGMN_13785 0.90 -0.2 2,737,590 + +1.4 2,737,591 - +0.1 2,737,591 - +1.3 2,737,595 + -1.8 2,737,595 + -0.2 2,737,595 + -2.3 2,737,622 + -1.3 2,737,623 - -0.6 2,737,623 - +0.3 2,737,634 + -1.2 2,737,634 + +2.0 2,737,635 - -0.2 2,737,666 + -2.4 2,737,666 + +0.8 2,737,666 + +1.0 2,737,688 + -1.8 2,737,688 + +0.3 2,737,689 - -1.3 2,737,689 - +0.7 2,737,689 - +0.2 2,737,718 + +2.6 2,737,726 - -3.2 2,737,736 + -2.3 2,737,736 + -1.9 2,737,736 + -0.4 2,737,736 + -2.2 2,737,737 - +2.4 2,737,737 - +0.9 2,737,737 - -1.6 2,737,737 - -1.2 2,737,742 + +1.1 2,737,742 + +0.7 2,737,742 + +0.4 2,737,743 - -2.2 2,737,743 - -2.0 2,737,743 - -1.9 2,737,851 - +1.4 2,737,925 + +1.2 2,737,934 - -0.2 2,738,042 + bisC NIAGMN_13790 0.12 -3.0 2,738,043 - bisC NIAGMN_13790 0.12 -2.4 2,738,057 + bisC NIAGMN_13790 0.13 +1.0 2,738,057 + bisC NIAGMN_13790 0.13 +0.4 2,738,057 + bisC NIAGMN_13790 0.13 -0.2 2,738,057 + bisC NIAGMN_13790 0.13 +2.7 2,738,057 + bisC NIAGMN_13790 0.13 +0.2 2,738,058 - bisC NIAGMN_13790 0.13 +0.4 2,738,076 + bisC NIAGMN_13790 0.13 +0.3 2,738,076 + bisC NIAGMN_13790 0.13 -1.2 2,738,076 + bisC NIAGMN_13790 0.13 -0.3 2,738,077 - bisC NIAGMN_13790 0.13 +3.7 2,738,136 - bisC NIAGMN_13790 0.16 -0.7 2,738,136 - bisC NIAGMN_13790 0.16 -0.5 2,738,149 + bisC NIAGMN_13790 0.16 +0.3 2,738,150 - bisC NIAGMN_13790 0.17 -1.1 2,738,152 + bisC NIAGMN_13790 0.17 -0.6 2,738,152 + bisC NIAGMN_13790 0.17 +0.4 2,738,152 + bisC NIAGMN_13790 0.17 +0.4 2,738,152 + bisC NIAGMN_13790 0.17 +2.7 2,738,152 + bisC NIAGMN_13790 0.17 +0.4 2,738,152 + bisC NIAGMN_13790 0.17 +0.3 2,738,153 - bisC NIAGMN_13790 0.17 +2.9 2,738,153 - bisC NIAGMN_13790 0.17 +2.2 2,738,153 - bisC NIAGMN_13790 0.17 +0.1 2,738,153 - bisC NIAGMN_13790 0.17 +2.6 2,738,153 - bisC NIAGMN_13790 0.17 -1.7 2,738,153 - bisC NIAGMN_13790 0.17 -2.0 2,738,160 + bisC NIAGMN_13790 0.17 +0.0 2,738,160 + bisC NIAGMN_13790 0.17 -3.0 2,738,160 + bisC NIAGMN_13790 0.17 +0.9 2,738,160 + bisC NIAGMN_13790 0.17 -0.8 2,738,160 + bisC NIAGMN_13790 0.17 +2.0 2,738,160 + bisC NIAGMN_13790 0.17 +2.5 2,738,160 + bisC NIAGMN_13790 0.17 +2.2 2,738,160 + bisC NIAGMN_13790 0.17 -3.4 2,738,160 + bisC NIAGMN_13790 0.17 +0.5 2,738,160 + bisC NIAGMN_13790 0.17 +0.2 2,738,160 + bisC NIAGMN_13790 0.17 +0.1 2,738,160 + bisC NIAGMN_13790 0.17 -2.2 2,738,161 - bisC NIAGMN_13790 0.17 +1.2 2,738,161 - bisC NIAGMN_13790 0.17 -0.2 2,738,161 - bisC NIAGMN_13790 0.17 -0.4 2,738,161 - bisC NIAGMN_13790 0.17 +0.0 2,738,161 - bisC NIAGMN_13790 0.17 -2.0 2,738,161 - bisC NIAGMN_13790 0.17 -2.6 2,738,161 - bisC NIAGMN_13790 0.17 -0.2 2,738,162 + bisC NIAGMN_13790 0.17 -0.8 2,738,162 + bisC NIAGMN_13790 0.17 -1.1 2,738,163 - bisC NIAGMN_13790 0.17 -2.6 2,738,181 + bisC NIAGMN_13790 0.18 -0.4 2,738,181 + bisC NIAGMN_13790 0.18 +0.3 2,738,181 + bisC NIAGMN_13790 0.18 -1.9 2,738,181 + bisC NIAGMN_13790 0.18 +0.9 2,738,183 + bisC NIAGMN_13790 0.18 -0.0 2,738,203 - bisC NIAGMN_13790 0.19 -2.5 2,738,235 + bisC NIAGMN_13790 0.20 +0.1 2,738,235 + bisC NIAGMN_13790 0.20 -0.7 2,738,235 + bisC NIAGMN_13790 0.20 +1.6 2,738,236 - bisC NIAGMN_13790 0.20 -0.3 2,738,236 - bisC NIAGMN_13790 0.20 +0.2 2,738,236 - bisC NIAGMN_13790 0.20 -3.0 2,738,249 + bisC NIAGMN_13790 0.21 +0.3 2,738,259 + bisC NIAGMN_13790 0.21 +0.4 2,738,298 - bisC NIAGMN_13790 0.23 -1.7 2,738,298 - bisC NIAGMN_13790 0.23 +1.3 2,738,337 - bisC NIAGMN_13790 0.25 -1.6 2,738,382 + bisC NIAGMN_13790 0.26 +1.7 2,738,529 - bisC NIAGMN_13790 0.33 +0.2 2,738,587 - bisC NIAGMN_13790 0.35 +1.5 2,738,604 + bisC NIAGMN_13790 0.36 -2.5 2,738,604 + bisC NIAGMN_13790 0.36 -2.0 2,738,605 - bisC NIAGMN_13790 0.36 -3.6 2,738,605 - bisC NIAGMN_13790 0.36 +2.6
Or see this region's nucleotide sequence