Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aslB and Pro_trna are separated by 146 nucleotides Pro_trna and Leu_trna are separated by 42 nucleotides Leu_trna and Pro_trna are separated by 20 nucleotides Pro_trna and Leu_trna are separated by 48 nucleotides Leu_trna and His_trna are separated by 20 nucleotides His_trna and Arg_trna are separated by 58 nucleotides Arg_trna and yifK are separated by 102 nucleotides
NIAGMN_12240: aslB - anaerobic sulfatase maturase, at 2,423,906 to 2,425,141
aslB
NIAGMN_12245: Pro_trna - tRNA-Pro(tgg), at 2,425,288 to 2,425,364
_trna
NIAGMN_12250: Leu_trna - tRNA-Leu(cag), at 2,425,407 to 2,425,493
_trna
NIAGMN_12255: Pro_trna - tRNA-Pro(tgg), at 2,425,514 to 2,425,584
_trna
NIAGMN_12260: Leu_trna - tRNA-Leu(cag), at 2,425,633 to 2,425,719
_trna
NIAGMN_12265: His_trna - tRNA-His(gtg), at 2,425,740 to 2,425,815
_trna
NIAGMN_12270: Arg_trna - tRNA-Arg(ccg), at 2,425,874 to 2,425,950
_trna
NIAGMN_12275: yifK - amino acid permease, at 2,426,053 to 2,427,438
yifK
Position (kb)
2425
2426 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2424.640 kb on + strand, within aslB at 2424.641 kb on - strand, within aslB at 2424.643 kb on - strand, within aslB at 2424.643 kb on - strand, within aslB at 2424.691 kb on + strand, within aslB at 2424.838 kb on + strand, within aslB at 2424.838 kb on + strand, within aslB at 2424.839 kb on - strand, within aslB at 2424.839 kb on - strand, within aslB at 2424.839 kb on - strand, within aslB at 2424.839 kb on - strand, within aslB at 2424.842 kb on + strand, within aslB at 2424.842 kb on + strand, within aslB at 2424.842 kb on + strand, within aslB at 2424.843 kb on - strand, within aslB at 2424.843 kb on - strand, within aslB at 2424.843 kb on - strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.861 kb on + strand, within aslB at 2424.862 kb on - strand, within aslB at 2424.862 kb on - strand, within aslB at 2424.862 kb on - strand, within aslB at 2424.862 kb on - strand, within aslB at 2424.862 kb on - strand, within aslB at 2424.970 kb on + strand, within aslB at 2424.970 kb on + strand, within aslB at 2424.970 kb on + strand, within aslB at 2424.970 kb on + strand, within aslB at 2424.971 kb on - strand, within aslB at 2424.971 kb on - strand, within aslB at 2424.971 kb on - strand, within aslB at 2424.971 kb on - strand, within aslB at 2424.971 kb on - strand, within aslB at 2424.972 kb on + strand, within aslB at 2424.972 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.984 kb on + strand, within aslB at 2424.985 kb on - strand, within aslB at 2425.010 kb on - strand, within aslB at 2425.010 kb on - strand, within aslB at 2425.062 kb on + strand at 2425.078 kb on + strand at 2425.173 kb on - strand at 2425.209 kb on + strand at 2425.209 kb on + strand at 2425.210 kb on - strand at 2425.212 kb on + strand at 2425.357 kb on - strand at 2425.369 kb on - strand at 2425.400 kb on - strand at 2425.400 kb on - strand at 2425.509 kb on - strand at 2425.509 kb on - strand at 2425.509 kb on - strand at 2425.509 kb on - strand at 2425.509 kb on - strand at 2425.509 kb on - strand at 2425.512 kb on - strand at 2425.512 kb on - strand at 2425.512 kb on - strand at 2425.512 kb on - strand at 2425.512 kb on - strand at 2426.054 kb on + strand at 2426.054 kb on + strand at 2426.054 kb on + strand at 2426.055 kb on - strand at 2426.103 kb on + strand at 2426.127 kb on + strand at 2426.154 kb on + strand at 2426.154 kb on + strand at 2426.155 kb on - strand at 2426.155 kb on - strand at 2426.155 kb on - strand at 2426.160 kb on + strand at 2426.160 kb on + strand at 2426.161 kb on - strand at 2426.169 kb on + strand at 2426.170 kb on - strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.174 kb on + strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.175 kb on - strand at 2426.213 kb on - strand, within yifK at 2426.213 kb on - strand, within yifK at 2426.213 kb on - strand, within yifK at 2426.213 kb on - strand, within yifK at 2426.213 kb on - strand, within yifK at 2426.216 kb on + strand, within yifK at 2426.216 kb on + strand, within yifK at 2426.216 kb on + strand, within yifK at 2426.216 kb on + strand, within yifK at 2426.216 kb on + strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.217 kb on - strand, within yifK at 2426.271 kb on + strand, within yifK at 2426.271 kb on + strand, within yifK at 2426.272 kb on - strand, within yifK at 2426.299 kb on + strand, within yifK at 2426.300 kb on - strand, within yifK at 2426.305 kb on + strand, within yifK at 2426.305 kb on + strand, within yifK at 2426.348 kb on + strand, within yifK at 2426.348 kb on + strand, within yifK at 2426.349 kb on - strand, within yifK at 2426.354 kb on + strand, within yifK at 2426.354 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.387 kb on + strand, within yifK at 2426.388 kb on - strand, within yifK at 2426.388 kb on - strand, within yifK at 2426.388 kb on - strand, within yifK at 2426.388 kb on - strand, within yifK at 2426.416 kb on - strand, within yifK at 2426.416 kb on - strand, within yifK at 2426.416 kb on - strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.421 kb on + strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.422 kb on - strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.425 kb on + strand, within yifK at 2426.426 kb on - strand, within yifK at 2426.426 kb on - strand, within yifK at 2426.426 kb on - strand, within yifK at 2426.483 kb on - strand, within yifK at 2426.507 kb on + strand, within yifK at 2426.508 kb on - strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.514 kb on + strand, within yifK at 2426.515 kb on - strand, within yifK at 2426.515 kb on - strand, within yifK at 2426.515 kb on - strand, within yifK at 2426.515 kb on - strand, within yifK at 2426.515 kb on - strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.528 kb on + strand, within yifK at 2426.529 kb on - strand, within yifK at 2426.542 kb on - strand, within yifK at 2426.581 kb on - strand, within yifK at 2426.647 kb on + strand, within yifK at 2426.647 kb on + strand, within yifK at 2426.647 kb on + strand, within yifK at 2426.647 kb on + strand, within yifK at 2426.648 kb on - strand, within yifK
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,424,640 + aslB NIAGMN_12240 0.59 +2.8 2,424,641 - aslB NIAGMN_12240 0.59 +1.1 2,424,643 - aslB NIAGMN_12240 0.60 +0.4 2,424,643 - aslB NIAGMN_12240 0.60 +0.7 2,424,691 + aslB NIAGMN_12240 0.64 -2.4 2,424,838 + aslB NIAGMN_12240 0.75 -2.6 2,424,838 + aslB NIAGMN_12240 0.75 +1.3 2,424,839 - aslB NIAGMN_12240 0.75 -2.2 2,424,839 - aslB NIAGMN_12240 0.75 +0.3 2,424,839 - aslB NIAGMN_12240 0.75 -1.3 2,424,839 - aslB NIAGMN_12240 0.75 -0.9 2,424,842 + aslB NIAGMN_12240 0.76 +1.1 2,424,842 + aslB NIAGMN_12240 0.76 -2.2 2,424,842 + aslB NIAGMN_12240 0.76 +0.6 2,424,843 - aslB NIAGMN_12240 0.76 -0.6 2,424,843 - aslB NIAGMN_12240 0.76 +1.1 2,424,843 - aslB NIAGMN_12240 0.76 +2.3 2,424,861 + aslB NIAGMN_12240 0.77 -2.4 2,424,861 + aslB NIAGMN_12240 0.77 +1.7 2,424,861 + aslB NIAGMN_12240 0.77 -2.6 2,424,861 + aslB NIAGMN_12240 0.77 +1.1 2,424,861 + aslB NIAGMN_12240 0.77 +1.7 2,424,861 + aslB NIAGMN_12240 0.77 +1.8 2,424,862 - aslB NIAGMN_12240 0.77 +0.2 2,424,862 - aslB NIAGMN_12240 0.77 +0.8 2,424,862 - aslB NIAGMN_12240 0.77 +1.3 2,424,862 - aslB NIAGMN_12240 0.77 +3.0 2,424,862 - aslB NIAGMN_12240 0.77 -0.6 2,424,970 + aslB NIAGMN_12240 0.86 +2.7 2,424,970 + aslB NIAGMN_12240 0.86 +1.8 2,424,970 + aslB NIAGMN_12240 0.86 -1.5 2,424,970 + aslB NIAGMN_12240 0.86 -2.0 2,424,971 - aslB NIAGMN_12240 0.86 +0.5 2,424,971 - aslB NIAGMN_12240 0.86 -1.8 2,424,971 - aslB NIAGMN_12240 0.86 +0.7 2,424,971 - aslB NIAGMN_12240 0.86 +0.9 2,424,971 - aslB NIAGMN_12240 0.86 -3.2 2,424,972 + aslB NIAGMN_12240 0.86 -0.9 2,424,972 + aslB NIAGMN_12240 0.86 -1.8 2,424,984 + aslB NIAGMN_12240 0.87 +0.3 2,424,984 + aslB NIAGMN_12240 0.87 -2.3 2,424,984 + aslB NIAGMN_12240 0.87 -1.1 2,424,984 + aslB NIAGMN_12240 0.87 -2.5 2,424,984 + aslB NIAGMN_12240 0.87 +0.6 2,424,984 + aslB NIAGMN_12240 0.87 +0.5 2,424,984 + aslB NIAGMN_12240 0.87 +0.4 2,424,985 - aslB NIAGMN_12240 0.87 +1.2 2,425,010 - aslB NIAGMN_12240 0.89 +0.4 2,425,010 - aslB NIAGMN_12240 0.89 +0.6 2,425,062 + -0.6 2,425,078 + -1.2 2,425,173 - -2.4 2,425,209 + +0.7 2,425,209 + -1.8 2,425,210 - -3.7 2,425,212 + -2.0 2,425,357 - +0.4 2,425,369 - +0.5 2,425,400 - +1.6 2,425,400 - +1.1 2,425,509 - -2.6 2,425,509 - +0.7 2,425,509 - +1.7 2,425,509 - +1.1 2,425,509 - -2.9 2,425,509 - +0.8 2,425,512 - +0.7 2,425,512 - -0.5 2,425,512 - -0.3 2,425,512 - +0.4 2,425,512 - -1.4 2,426,054 + +2.2 2,426,054 + -1.0 2,426,054 + -0.2 2,426,055 - -1.1 2,426,103 + -0.3 2,426,127 + +2.4 2,426,154 + -1.8 2,426,154 + -3.4 2,426,155 - -0.8 2,426,155 - +0.2 2,426,155 - -3.1 2,426,160 + -0.7 2,426,160 + -1.1 2,426,161 - -0.9 2,426,169 + +0.7 2,426,170 - -1.5 2,426,174 + +1.8 2,426,174 + -0.8 2,426,174 + +2.1 2,426,174 + -2.2 2,426,174 + -1.3 2,426,174 + -1.0 2,426,174 + -1.4 2,426,175 - -0.3 2,426,175 - +1.1 2,426,175 - -0.4 2,426,175 - -3.5 2,426,175 - -0.5 2,426,175 - -3.5 2,426,175 - -1.2 2,426,213 - yifK NIAGMN_12275 0.12 +1.4 2,426,213 - yifK NIAGMN_12275 0.12 +1.4 2,426,213 - yifK NIAGMN_12275 0.12 -1.6 2,426,213 - yifK NIAGMN_12275 0.12 -2.6 2,426,213 - yifK NIAGMN_12275 0.12 +0.2 2,426,216 + yifK NIAGMN_12275 0.12 -0.9 2,426,216 + yifK NIAGMN_12275 0.12 +0.6 2,426,216 + yifK NIAGMN_12275 0.12 -3.2 2,426,216 + yifK NIAGMN_12275 0.12 +1.5 2,426,216 + yifK NIAGMN_12275 0.12 +0.5 2,426,217 - yifK NIAGMN_12275 0.12 +4.6 2,426,217 - yifK NIAGMN_12275 0.12 +2.9 2,426,217 - yifK NIAGMN_12275 0.12 +2.1 2,426,217 - yifK NIAGMN_12275 0.12 +2.3 2,426,217 - yifK NIAGMN_12275 0.12 -0.6 2,426,217 - yifK NIAGMN_12275 0.12 +1.3 2,426,217 - yifK NIAGMN_12275 0.12 -2.7 2,426,217 - yifK NIAGMN_12275 0.12 +0.0 2,426,271 + yifK NIAGMN_12275 0.16 +1.3 2,426,271 + yifK NIAGMN_12275 0.16 +0.2 2,426,272 - yifK NIAGMN_12275 0.16 +0.5 2,426,299 + yifK NIAGMN_12275 0.18 +1.7 2,426,300 - yifK NIAGMN_12275 0.18 -1.7 2,426,305 + yifK NIAGMN_12275 0.18 -1.7 2,426,305 + yifK NIAGMN_12275 0.18 +0.3 2,426,348 + yifK NIAGMN_12275 0.21 -2.0 2,426,348 + yifK NIAGMN_12275 0.21 +0.6 2,426,349 - yifK NIAGMN_12275 0.21 -1.6 2,426,354 + yifK NIAGMN_12275 0.22 +0.7 2,426,354 + yifK NIAGMN_12275 0.22 +1.8 2,426,387 + yifK NIAGMN_12275 0.24 -0.1 2,426,387 + yifK NIAGMN_12275 0.24 -0.6 2,426,387 + yifK NIAGMN_12275 0.24 -0.9 2,426,387 + yifK NIAGMN_12275 0.24 +0.8 2,426,387 + yifK NIAGMN_12275 0.24 +0.3 2,426,387 + yifK NIAGMN_12275 0.24 -0.8 2,426,388 - yifK NIAGMN_12275 0.24 -0.6 2,426,388 - yifK NIAGMN_12275 0.24 +0.1 2,426,388 - yifK NIAGMN_12275 0.24 -0.2 2,426,388 - yifK NIAGMN_12275 0.24 -4.3 2,426,416 - yifK NIAGMN_12275 0.26 +0.4 2,426,416 - yifK NIAGMN_12275 0.26 +0.4 2,426,416 - yifK NIAGMN_12275 0.26 +1.1 2,426,421 + yifK NIAGMN_12275 0.27 +1.5 2,426,421 + yifK NIAGMN_12275 0.27 -0.9 2,426,421 + yifK NIAGMN_12275 0.27 +0.3 2,426,421 + yifK NIAGMN_12275 0.27 -0.2 2,426,421 + yifK NIAGMN_12275 0.27 -1.0 2,426,421 + yifK NIAGMN_12275 0.27 +0.1 2,426,421 + yifK NIAGMN_12275 0.27 -1.7 2,426,421 + yifK NIAGMN_12275 0.27 -0.0 2,426,421 + yifK NIAGMN_12275 0.27 -0.3 2,426,421 + yifK NIAGMN_12275 0.27 -3.4 2,426,421 + yifK NIAGMN_12275 0.27 -1.7 2,426,421 + yifK NIAGMN_12275 0.27 +2.0 2,426,421 + yifK NIAGMN_12275 0.27 -0.4 2,426,421 + yifK NIAGMN_12275 0.27 -0.0 2,426,421 + yifK NIAGMN_12275 0.27 +2.1 2,426,422 - yifK NIAGMN_12275 0.27 -0.3 2,426,422 - yifK NIAGMN_12275 0.27 +2.0 2,426,422 - yifK NIAGMN_12275 0.27 -2.9 2,426,422 - yifK NIAGMN_12275 0.27 +2.2 2,426,422 - yifK NIAGMN_12275 0.27 +0.4 2,426,422 - yifK NIAGMN_12275 0.27 +0.7 2,426,425 + yifK NIAGMN_12275 0.27 +0.7 2,426,425 + yifK NIAGMN_12275 0.27 -1.1 2,426,425 + yifK NIAGMN_12275 0.27 +2.2 2,426,425 + yifK NIAGMN_12275 0.27 -1.1 2,426,425 + yifK NIAGMN_12275 0.27 -2.1 2,426,425 + yifK NIAGMN_12275 0.27 +0.8 2,426,426 - yifK NIAGMN_12275 0.27 +1.8 2,426,426 - yifK NIAGMN_12275 0.27 -0.1 2,426,426 - yifK NIAGMN_12275 0.27 +0.4 2,426,483 - yifK NIAGMN_12275 0.31 +0.3 2,426,507 + yifK NIAGMN_12275 0.33 +0.4 2,426,508 - yifK NIAGMN_12275 0.33 -0.0 2,426,514 + yifK NIAGMN_12275 0.33 -0.3 2,426,514 + yifK NIAGMN_12275 0.33 -2.5 2,426,514 + yifK NIAGMN_12275 0.33 -0.8 2,426,514 + yifK NIAGMN_12275 0.33 +0.0 2,426,514 + yifK NIAGMN_12275 0.33 +0.2 2,426,514 + yifK NIAGMN_12275 0.33 -0.1 2,426,514 + yifK NIAGMN_12275 0.33 -1.8 2,426,515 - yifK NIAGMN_12275 0.33 +1.5 2,426,515 - yifK NIAGMN_12275 0.33 +3.0 2,426,515 - yifK NIAGMN_12275 0.33 +3.8 2,426,515 - yifK NIAGMN_12275 0.33 -1.2 2,426,515 - yifK NIAGMN_12275 0.33 +1.6 2,426,528 + yifK NIAGMN_12275 0.34 +0.8 2,426,528 + yifK NIAGMN_12275 0.34 +0.2 2,426,528 + yifK NIAGMN_12275 0.34 -1.3 2,426,528 + yifK NIAGMN_12275 0.34 +0.2 2,426,528 + yifK NIAGMN_12275 0.34 +2.6 2,426,528 + yifK NIAGMN_12275 0.34 +0.9 2,426,529 - yifK NIAGMN_12275 0.34 -0.4 2,426,542 - yifK NIAGMN_12275 0.35 -0.8 2,426,581 - yifK NIAGMN_12275 0.38 +0.4 2,426,647 + yifK NIAGMN_12275 0.43 -0.8 2,426,647 + yifK NIAGMN_12275 0.43 -1.3 2,426,647 + yifK NIAGMN_12275 0.43 -1.0 2,426,647 + yifK NIAGMN_12275 0.43 +2.2 2,426,648 - yifK NIAGMN_12275 0.43 -0.9
Or see this region's nucleotide sequence