Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_11745 and fabY are separated by 410 nucleotides fabY and dtd are separated by 44 nucleotides dtd and yihY overlap by 4 nucleotides
NIAGMN_11745: NIAGMN_11745 - hypothetical protein, at 2,325,411 to 2,326,304
_11745
NIAGMN_11750: fabY - fatty acid biosynthesis protein FabY, at 2,326,715 to 2,327,656
fabY
NIAGMN_11755: dtd - D-aminoacyl-tRNA deacylase, at 2,327,701 to 2,328,138
dtd
NIAGMN_11760: yihY - virulence factor BrkB family protein, at 2,328,135 to 2,329,007
yihY
Position (kb)
2326
2327
2328 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2325.735 kb on + strand, within NIAGMN_11745 at 2325.736 kb on - strand, within NIAGMN_11745 at 2325.753 kb on - strand, within NIAGMN_11745 at 2325.784 kb on + strand, within NIAGMN_11745 at 2325.837 kb on + strand, within NIAGMN_11745 at 2325.839 kb on + strand, within NIAGMN_11745 at 2326.027 kb on + strand, within NIAGMN_11745 at 2326.029 kb on + strand, within NIAGMN_11745 at 2326.293 kb on - strand at 2326.341 kb on + strand at 2326.341 kb on + strand at 2326.346 kb on - strand at 2326.431 kb on - strand at 2326.566 kb on + strand at 2326.589 kb on - strand at 2326.589 kb on - strand at 2326.670 kb on - strand at 2326.671 kb on + strand at 2326.671 kb on + strand at 2326.671 kb on + strand at 2326.672 kb on - strand at 2326.717 kb on - strand at 2326.744 kb on - strand at 2326.774 kb on + strand at 2326.774 kb on + strand at 2326.776 kb on + strand at 2326.776 kb on + strand at 2326.776 kb on + strand at 2326.776 kb on + strand at 2326.776 kb on + strand at 2326.776 kb on + strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2326.777 kb on - strand at 2327.053 kb on + strand, within fabY at 2327.053 kb on + strand, within fabY at 2327.053 kb on + strand, within fabY at 2327.054 kb on - strand, within fabY at 2327.054 kb on - strand, within fabY at 2327.054 kb on - strand, within fabY at 2327.089 kb on + strand, within fabY at 2327.089 kb on + strand, within fabY at 2327.089 kb on + strand, within fabY at 2327.089 kb on + strand, within fabY at 2327.110 kb on + strand, within fabY at 2327.110 kb on + strand, within fabY at 2327.111 kb on - strand, within fabY at 2327.113 kb on - strand, within fabY at 2327.113 kb on - strand, within fabY at 2327.144 kb on - strand, within fabY at 2327.153 kb on - strand, within fabY at 2327.153 kb on - strand, within fabY at 2327.207 kb on - strand, within fabY at 2327.230 kb on + strand, within fabY at 2327.230 kb on + strand, within fabY at 2327.230 kb on + strand, within fabY at 2327.288 kb on - strand, within fabY at 2327.437 kb on + strand, within fabY at 2327.467 kb on + strand, within fabY at 2327.468 kb on - strand, within fabY at 2327.468 kb on - strand, within fabY at 2327.470 kb on + strand, within fabY at 2327.470 kb on + strand, within fabY at 2327.471 kb on - strand, within fabY at 2327.471 kb on - strand, within fabY at 2327.471 kb on - strand, within fabY at 2327.471 kb on - strand, within fabY at 2327.472 kb on + strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.473 kb on - strand, within fabY at 2327.497 kb on + strand, within fabY at 2327.498 kb on - strand, within fabY at 2327.610 kb on + strand at 2327.611 kb on - strand at 2327.618 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.652 kb on + strand at 2327.653 kb on - strand at 2327.653 kb on - strand at 2327.653 kb on - strand at 2327.656 kb on + strand at 2327.657 kb on - strand at 2327.657 kb on - strand at 2327.663 kb on - strand at 2327.704 kb on + strand at 2327.747 kb on - strand, within dtd at 2327.816 kb on + strand, within dtd at 2327.816 kb on + strand, within dtd at 2327.817 kb on - strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.822 kb on + strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.823 kb on - strand, within dtd at 2327.824 kb on + strand, within dtd at 2327.824 kb on + strand, within dtd at 2327.824 kb on + strand, within dtd at 2327.824 kb on + strand, within dtd at 2327.825 kb on - strand, within dtd at 2327.825 kb on - strand, within dtd at 2327.825 kb on - strand, within dtd at 2327.902 kb on - strand, within dtd at 2328.049 kb on + strand, within dtd at 2328.050 kb on - strand, within dtd at 2328.116 kb on - strand at 2328.127 kb on + strand at 2328.128 kb on - strand at 2328.128 kb on - strand at 2328.174 kb on + strand at 2328.174 kb on + strand at 2328.185 kb on - strand at 2328.203 kb on + strand at 2328.204 kb on - strand at 2328.227 kb on + strand, within yihY at 2328.227 kb on + strand, within yihY at 2328.227 kb on + strand, within yihY at 2328.284 kb on - strand, within yihY at 2328.284 kb on - strand, within yihY at 2328.315 kb on - strand, within yihY at 2328.317 kb on - strand, within yihY at 2328.393 kb on + strand, within yihY at 2328.418 kb on + strand, within yihY at 2328.485 kb on + strand, within yihY at 2328.485 kb on + strand, within yihY at 2328.485 kb on + strand, within yihY at 2328.485 kb on + strand, within yihY at 2328.485 kb on + strand, within yihY at 2328.520 kb on + strand, within yihY at 2328.521 kb on - strand, within yihY at 2328.568 kb on - strand, within yihY at 2328.568 kb on - strand, within yihY at 2328.580 kb on + strand, within yihY at 2328.581 kb on - strand, within yihY at 2328.581 kb on - strand, within yihY at 2328.581 kb on - strand, within yihY at 2328.632 kb on + strand, within yihY at 2328.633 kb on - strand, within yihY at 2328.633 kb on - strand, within yihY at 2328.656 kb on - strand, within yihY at 2328.656 kb on - strand, within yihY at 2328.656 kb on - strand, within yihY at 2328.656 kb on - strand, within yihY
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,325,735 + NIAGMN_11745 0.36 -0.0 2,325,736 - NIAGMN_11745 0.36 +0.7 2,325,753 - NIAGMN_11745 0.38 -0.6 2,325,784 + NIAGMN_11745 0.42 -4.0 2,325,837 + NIAGMN_11745 0.48 -2.4 2,325,839 + NIAGMN_11745 0.48 -1.8 2,326,027 + NIAGMN_11745 0.69 -3.0 2,326,029 + NIAGMN_11745 0.69 -3.0 2,326,293 - +0.8 2,326,341 + +1.3 2,326,341 + -0.6 2,326,346 - +1.4 2,326,431 - +0.1 2,326,566 + +0.6 2,326,589 - -0.9 2,326,589 - +2.3 2,326,670 - +3.7 2,326,671 + +2.2 2,326,671 + +0.6 2,326,671 + +1.4 2,326,672 - +0.8 2,326,717 - -1.0 2,326,744 - +0.4 2,326,774 + +0.2 2,326,774 + +2.4 2,326,776 + +1.1 2,326,776 + +0.5 2,326,776 + +0.4 2,326,776 + -1.4 2,326,776 + -0.6 2,326,776 + -1.3 2,326,777 - -1.5 2,326,777 - +0.4 2,326,777 - +0.2 2,326,777 - +0.1 2,326,777 - +1.0 2,326,777 - +1.2 2,326,777 - +2.4 2,327,053 + fabY NIAGMN_11750 0.36 +0.7 2,327,053 + fabY NIAGMN_11750 0.36 -0.5 2,327,053 + fabY NIAGMN_11750 0.36 -0.4 2,327,054 - fabY NIAGMN_11750 0.36 -0.8 2,327,054 - fabY NIAGMN_11750 0.36 -2.4 2,327,054 - fabY NIAGMN_11750 0.36 -0.1 2,327,089 + fabY NIAGMN_11750 0.40 +0.2 2,327,089 + fabY NIAGMN_11750 0.40 -0.7 2,327,089 + fabY NIAGMN_11750 0.40 +0.1 2,327,089 + fabY NIAGMN_11750 0.40 -0.7 2,327,110 + fabY NIAGMN_11750 0.42 +1.3 2,327,110 + fabY NIAGMN_11750 0.42 +1.8 2,327,111 - fabY NIAGMN_11750 0.42 -0.1 2,327,113 - fabY NIAGMN_11750 0.42 +0.4 2,327,113 - fabY NIAGMN_11750 0.42 +1.4 2,327,144 - fabY NIAGMN_11750 0.46 +0.3 2,327,153 - fabY NIAGMN_11750 0.46 +1.6 2,327,153 - fabY NIAGMN_11750 0.46 +0.0 2,327,207 - fabY NIAGMN_11750 0.52 +0.2 2,327,230 + fabY NIAGMN_11750 0.55 -0.3 2,327,230 + fabY NIAGMN_11750 0.55 +1.0 2,327,230 + fabY NIAGMN_11750 0.55 -0.0 2,327,288 - fabY NIAGMN_11750 0.61 +1.6 2,327,437 + fabY NIAGMN_11750 0.77 -0.1 2,327,467 + fabY NIAGMN_11750 0.80 -0.0 2,327,468 - fabY NIAGMN_11750 0.80 -0.3 2,327,468 - fabY NIAGMN_11750 0.80 +1.3 2,327,470 + fabY NIAGMN_11750 0.80 +0.2 2,327,470 + fabY NIAGMN_11750 0.80 -0.0 2,327,471 - fabY NIAGMN_11750 0.80 -0.4 2,327,471 - fabY NIAGMN_11750 0.80 -1.5 2,327,471 - fabY NIAGMN_11750 0.80 +0.0 2,327,471 - fabY NIAGMN_11750 0.80 -0.7 2,327,472 + fabY NIAGMN_11750 0.80 +0.2 2,327,473 - fabY NIAGMN_11750 0.80 -1.3 2,327,473 - fabY NIAGMN_11750 0.80 -1.9 2,327,473 - fabY NIAGMN_11750 0.80 +1.0 2,327,473 - fabY NIAGMN_11750 0.80 +0.2 2,327,473 - fabY NIAGMN_11750 0.80 -0.4 2,327,473 - fabY NIAGMN_11750 0.80 +0.8 2,327,497 + fabY NIAGMN_11750 0.83 -1.8 2,327,498 - fabY NIAGMN_11750 0.83 +1.1 2,327,610 + +2.3 2,327,611 - +0.6 2,327,618 + +0.2 2,327,652 + -1.8 2,327,652 + +1.1 2,327,652 + -1.4 2,327,652 + +0.2 2,327,652 + -2.1 2,327,652 + -1.8 2,327,652 + -0.4 2,327,653 - -1.5 2,327,653 - +1.3 2,327,653 - -0.7 2,327,656 + -0.5 2,327,657 - +2.7 2,327,657 - +1.8 2,327,663 - -1.8 2,327,704 + -0.2 2,327,747 - dtd NIAGMN_11755 0.11 -2.2 2,327,816 + dtd NIAGMN_11755 0.26 -0.6 2,327,816 + dtd NIAGMN_11755 0.26 +2.3 2,327,817 - dtd NIAGMN_11755 0.26 +1.1 2,327,822 + dtd NIAGMN_11755 0.28 +1.3 2,327,822 + dtd NIAGMN_11755 0.28 +2.2 2,327,822 + dtd NIAGMN_11755 0.28 +0.7 2,327,822 + dtd NIAGMN_11755 0.28 +1.5 2,327,822 + dtd NIAGMN_11755 0.28 +1.3 2,327,822 + dtd NIAGMN_11755 0.28 +1.4 2,327,822 + dtd NIAGMN_11755 0.28 -0.2 2,327,822 + dtd NIAGMN_11755 0.28 -0.4 2,327,822 + dtd NIAGMN_11755 0.28 +1.4 2,327,822 + dtd NIAGMN_11755 0.28 -0.0 2,327,822 + dtd NIAGMN_11755 0.28 +0.0 2,327,822 + dtd NIAGMN_11755 0.28 -1.0 2,327,823 - dtd NIAGMN_11755 0.28 -2.5 2,327,823 - dtd NIAGMN_11755 0.28 -0.2 2,327,823 - dtd NIAGMN_11755 0.28 -0.6 2,327,823 - dtd NIAGMN_11755 0.28 -2.3 2,327,823 - dtd NIAGMN_11755 0.28 +2.5 2,327,823 - dtd NIAGMN_11755 0.28 -1.3 2,327,823 - dtd NIAGMN_11755 0.28 -2.1 2,327,823 - dtd NIAGMN_11755 0.28 +1.7 2,327,824 + dtd NIAGMN_11755 0.28 +2.9 2,327,824 + dtd NIAGMN_11755 0.28 -2.5 2,327,824 + dtd NIAGMN_11755 0.28 -0.1 2,327,824 + dtd NIAGMN_11755 0.28 +1.6 2,327,825 - dtd NIAGMN_11755 0.28 -0.2 2,327,825 - dtd NIAGMN_11755 0.28 -0.9 2,327,825 - dtd NIAGMN_11755 0.28 +0.6 2,327,902 - dtd NIAGMN_11755 0.46 -0.0 2,328,049 + dtd NIAGMN_11755 0.79 +0.2 2,328,050 - dtd NIAGMN_11755 0.80 +2.3 2,328,116 - +1.9 2,328,127 + +0.5 2,328,128 - -0.8 2,328,128 - -2.9 2,328,174 + +0.8 2,328,174 + +1.3 2,328,185 - +0.1 2,328,203 + -0.5 2,328,204 - +0.8 2,328,227 + yihY NIAGMN_11760 0.11 +0.5 2,328,227 + yihY NIAGMN_11760 0.11 -0.5 2,328,227 + yihY NIAGMN_11760 0.11 -0.5 2,328,284 - yihY NIAGMN_11760 0.17 -0.9 2,328,284 - yihY NIAGMN_11760 0.17 +0.5 2,328,315 - yihY NIAGMN_11760 0.21 +0.5 2,328,317 - yihY NIAGMN_11760 0.21 -0.6 2,328,393 + yihY NIAGMN_11760 0.30 +0.7 2,328,418 + yihY NIAGMN_11760 0.32 -2.2 2,328,485 + yihY NIAGMN_11760 0.40 +1.0 2,328,485 + yihY NIAGMN_11760 0.40 +1.2 2,328,485 + yihY NIAGMN_11760 0.40 +1.0 2,328,485 + yihY NIAGMN_11760 0.40 +0.0 2,328,485 + yihY NIAGMN_11760 0.40 +2.6 2,328,520 + yihY NIAGMN_11760 0.44 -0.3 2,328,521 - yihY NIAGMN_11760 0.44 -0.1 2,328,568 - yihY NIAGMN_11760 0.50 -1.7 2,328,568 - yihY NIAGMN_11760 0.50 +0.5 2,328,580 + yihY NIAGMN_11760 0.51 +0.4 2,328,581 - yihY NIAGMN_11760 0.51 +0.0 2,328,581 - yihY NIAGMN_11760 0.51 +0.9 2,328,581 - yihY NIAGMN_11760 0.51 -1.3 2,328,632 + yihY NIAGMN_11760 0.57 -0.3 2,328,633 - yihY NIAGMN_11760 0.57 +1.2 2,328,633 - yihY NIAGMN_11760 0.57 +1.2 2,328,656 - yihY NIAGMN_11760 0.60 +0.4 2,328,656 - yihY NIAGMN_11760 0.60 +2.6 2,328,656 - yihY NIAGMN_11760 0.60 -0.6 2,328,656 - yihY NIAGMN_11760 0.60 +0.9
Or see this region's nucleotide sequence