Strain Fitness in Escherichia coli ECRC102 around NIAGMN_11250

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurB and 5S_rrna are separated by 136 nucleotides5S_rrna and 23S_rrna are separated by 94 nucleotides NIAGMN_11245: murB - UDP-N-acetylmuramate dehydrogenase, at 2,219,622 to 2,220,650 murB NIAGMN_11250: 5S_rrna - 5S ribosomal RNA, at 2,220,787 to 2,220,902 _rrna NIAGMN_11255: 23S_rrna - 23S ribosomal RNA, at 2,220,997 to 2,223,901 _rrna Position (kb) 2220 2221Strain fitness (log2 ratio) -2 -1 0 1at 2220.680 kb on - strandat 2220.680 kb on - strandat 2220.710 kb on + strandat 2220.711 kb on - strandat 2220.714 kb on + strandat 2220.736 kb on - strandat 2220.802 kb on - strand, within 5S_rrna

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,220,680 - -1.4
2,220,680 - +0.6
2,220,710 + +0.6
2,220,711 - +0.7
2,220,714 + +1.3
2,220,736 - -0.6
2,220,802 - 5S_rrna NIAGMN_11250 0.13 -2.6

Or see this region's nucleotide sequence