Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnD and NIAGMN_10485 are separated by 105 nucleotides NIAGMN_10485 and phnF are separated by 20 nucleotides phnF and phnG are separated by 0 nucleotides
NIAGMN_10475: phnD - phosphonate ABC transporter substrate-binding protein, at 2,061,285 to 2,062,301
phnD
NIAGMN_10485: NIAGMN_10485 - Phosphonate ABC transporter permease protein PhnE, at 2,062,407 to 2,063,186
_10485
NIAGMN_10490: phnF - phosphonate metabolism transcriptional regulator PhnF, at 2,063,207 to 2,063,932
phnF
NIAGMN_10495: phnG - phosphonate C-P lyase system protein PhnG, at 2,063,933 to 2,064,385
phnG
Position (kb)
2062
2063
2064 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2061.446 kb on - strand, within phnD at 2061.502 kb on - strand, within phnD at 2061.772 kb on - strand, within phnD at 2061.772 kb on - strand, within phnD at 2061.772 kb on - strand, within phnD at 2061.791 kb on + strand, within phnD at 2061.992 kb on + strand, within phnD at 2062.026 kb on + strand, within phnD at 2062.027 kb on - strand, within phnD at 2062.178 kb on + strand, within phnD at 2062.178 kb on + strand, within phnD at 2062.179 kb on - strand, within phnD at 2062.179 kb on - strand, within phnD at 2062.283 kb on - strand at 2062.286 kb on + strand at 2062.286 kb on + strand at 2062.286 kb on + strand at 2062.286 kb on + strand at 2062.286 kb on + strand at 2062.286 kb on + strand at 2062.287 kb on - strand at 2062.599 kb on + strand, within NIAGMN_10485 at 2062.599 kb on + strand, within NIAGMN_10485 at 2062.600 kb on - strand, within NIAGMN_10485 at 2062.600 kb on - strand, within NIAGMN_10485 at 2062.600 kb on - strand, within NIAGMN_10485 at 2062.600 kb on - strand, within NIAGMN_10485 at 2062.600 kb on - strand, within NIAGMN_10485 at 2062.717 kb on - strand, within NIAGMN_10485 at 2062.948 kb on - strand, within NIAGMN_10485 at 2062.986 kb on + strand, within NIAGMN_10485 at 2062.986 kb on + strand, within NIAGMN_10485 at 2062.987 kb on - strand, within NIAGMN_10485 at 2063.171 kb on + strand at 2063.180 kb on + strand at 2063.181 kb on - strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.202 kb on + strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.203 kb on - strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.206 kb on + strand at 2063.207 kb on - strand at 2063.207 kb on - strand at 2063.207 kb on - strand at 2063.207 kb on - strand at 2063.207 kb on - strand at 2063.207 kb on - strand at 2063.218 kb on + strand at 2063.246 kb on + strand at 2063.247 kb on - strand at 2063.306 kb on + strand, within phnF at 2063.307 kb on - strand, within phnF at 2063.400 kb on + strand, within phnF at 2063.640 kb on + strand, within phnF at 2063.651 kb on + strand, within phnF at 2063.652 kb on - strand, within phnF at 2063.815 kb on + strand, within phnF at 2063.816 kb on - strand, within phnF at 2063.880 kb on - strand at 2063.945 kb on - strand at 2063.945 kb on - strand at 2064.038 kb on + strand, within phnG at 2064.038 kb on + strand, within phnG at 2064.038 kb on + strand, within phnG at 2064.038 kb on + strand, within phnG at 2064.039 kb on - strand, within phnG at 2064.039 kb on - strand, within phnG at 2064.039 kb on - strand, within phnG at 2064.039 kb on - strand, within phnG at 2064.147 kb on + strand, within phnG at 2064.147 kb on + strand, within phnG at 2064.147 kb on + strand, within phnG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,061,446 - phnD NIAGMN_10475 0.16 -0.5 2,061,502 - phnD NIAGMN_10475 0.21 -0.4 2,061,772 - phnD NIAGMN_10475 0.48 +3.0 2,061,772 - phnD NIAGMN_10475 0.48 -1.2 2,061,772 - phnD NIAGMN_10475 0.48 -1.2 2,061,791 + phnD NIAGMN_10475 0.50 -1.2 2,061,992 + phnD NIAGMN_10475 0.70 -3.5 2,062,026 + phnD NIAGMN_10475 0.73 +0.3 2,062,027 - phnD NIAGMN_10475 0.73 +0.6 2,062,178 + phnD NIAGMN_10475 0.88 +1.2 2,062,178 + phnD NIAGMN_10475 0.88 -3.6 2,062,179 - phnD NIAGMN_10475 0.88 +1.0 2,062,179 - phnD NIAGMN_10475 0.88 -0.4 2,062,283 - +0.3 2,062,286 + +0.8 2,062,286 + +0.4 2,062,286 + +2.6 2,062,286 + +0.2 2,062,286 + -0.5 2,062,286 + -0.5 2,062,287 - -3.4 2,062,599 + NIAGMN_10485 0.25 +1.0 2,062,599 + NIAGMN_10485 0.25 -0.8 2,062,600 - NIAGMN_10485 0.25 -2.5 2,062,600 - NIAGMN_10485 0.25 -1.2 2,062,600 - NIAGMN_10485 0.25 +2.6 2,062,600 - NIAGMN_10485 0.25 -1.4 2,062,600 - NIAGMN_10485 0.25 +1.3 2,062,717 - NIAGMN_10485 0.40 -0.0 2,062,948 - NIAGMN_10485 0.69 -2.3 2,062,986 + NIAGMN_10485 0.74 -0.9 2,062,986 + NIAGMN_10485 0.74 +0.2 2,062,987 - NIAGMN_10485 0.74 +1.3 2,063,171 + +1.9 2,063,180 + -1.2 2,063,181 - +0.3 2,063,202 + +0.2 2,063,202 + +0.2 2,063,202 + -3.0 2,063,202 + -3.5 2,063,202 + -1.8 2,063,202 + -0.9 2,063,202 + -0.1 2,063,202 + +0.5 2,063,203 - -0.5 2,063,203 - +0.6 2,063,203 - -0.9 2,063,203 - -0.5 2,063,203 - -0.7 2,063,203 - +0.6 2,063,203 - -0.5 2,063,206 + -3.2 2,063,206 + -0.2 2,063,206 + -0.5 2,063,206 + +1.1 2,063,206 + -0.5 2,063,206 + -2.7 2,063,206 + +0.8 2,063,206 + +0.8 2,063,207 - +0.3 2,063,207 - +3.2 2,063,207 - +0.8 2,063,207 - +0.9 2,063,207 - -1.9 2,063,207 - -2.8 2,063,218 + +0.6 2,063,246 + +0.8 2,063,247 - +0.8 2,063,306 + phnF NIAGMN_10490 0.14 -0.2 2,063,307 - phnF NIAGMN_10490 0.14 -0.7 2,063,400 + phnF NIAGMN_10490 0.27 -0.5 2,063,640 + phnF NIAGMN_10490 0.60 -3.2 2,063,651 + phnF NIAGMN_10490 0.61 +0.7 2,063,652 - phnF NIAGMN_10490 0.61 +0.2 2,063,815 + phnF NIAGMN_10490 0.84 +0.6 2,063,816 - phnF NIAGMN_10490 0.84 -0.9 2,063,880 - +0.6 2,063,945 - +2.4 2,063,945 - +0.8 2,064,038 + phnG NIAGMN_10495 0.23 +1.3 2,064,038 + phnG NIAGMN_10495 0.23 -0.7 2,064,038 + phnG NIAGMN_10495 0.23 -2.0 2,064,038 + phnG NIAGMN_10495 0.23 +0.9 2,064,039 - phnG NIAGMN_10495 0.23 +0.4 2,064,039 - phnG NIAGMN_10495 0.23 +1.2 2,064,039 - phnG NIAGMN_10495 0.23 -2.7 2,064,039 - phnG NIAGMN_10495 0.23 -2.1 2,064,147 + phnG NIAGMN_10495 0.47 +0.8 2,064,147 + phnG NIAGMN_10495 0.47 -0.3 2,064,147 + phnG NIAGMN_10495 0.47 -2.6
Or see this region's nucleotide sequence