Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsgA and asd are separated by 96 nucleotides asd and mscM are separated by 21 nucleotides
NIAGMN_10165: rsgA - small ribosomal subunit biogenesis GTPase RsgA, at 1,995,250 to 1,996,302
rsgA
NIAGMN_10170: asd - archaetidylserine decarboxylase, at 1,996,399 to 1,997,367
asd
NIAGMN_10175: mscM - miniconductance mechanosensitive channel MscM, at 1,997,389 to 2,000,712
mscM
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1996.242 kb on + strand at 1996.291 kb on - strand at 1996.307 kb on + strand at 1996.307 kb on + strand at 1996.307 kb on + strand at 1996.307 kb on + strand at 1996.307 kb on + strand at 1996.307 kb on + strand at 1996.312 kb on + strand at 1996.312 kb on + strand at 1996.322 kb on + strand at 1996.356 kb on + strand at 1997.252 kb on + strand, within asd at 1997.252 kb on + strand, within asd at 1997.252 kb on + strand, within asd at 1997.257 kb on - strand, within asd at 1997.271 kb on + strand at 1997.365 kb on + strand at 1997.366 kb on - strand at 1997.400 kb on + strand at 1997.441 kb on - strand at 1997.460 kb on + strand at 1997.460 kb on + strand at 1997.547 kb on - strand at 1997.611 kb on - strand at 1997.615 kb on - strand at 1997.649 kb on - strand at 1997.745 kb on + strand, within mscM at 1997.745 kb on + strand, within mscM at 1997.843 kb on + strand, within mscM at 1997.844 kb on - strand, within mscM at 1997.844 kb on - strand, within mscM at 1997.883 kb on + strand, within mscM at 1997.884 kb on - strand, within mscM at 1997.884 kb on - strand, within mscM at 1997.884 kb on - strand, within mscM at 1997.938 kb on - strand, within mscM at 1997.938 kb on - strand, within mscM at 1997.938 kb on - strand, within mscM at 1997.938 kb on - strand, within mscM at 1997.996 kb on + strand, within mscM at 1997.996 kb on + strand, within mscM at 1998.049 kb on + strand, within mscM at 1998.049 kb on + strand, within mscM at 1998.049 kb on + strand, within mscM at 1998.050 kb on - strand, within mscM at 1998.126 kb on - strand, within mscM at 1998.160 kb on - strand, within mscM at 1998.180 kb on + strand, within mscM at 1998.291 kb on + strand, within mscM at 1998.291 kb on + strand, within mscM at 1998.292 kb on - strand, within mscM at 1998.292 kb on - strand, within mscM
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,996,242 + -1.8 1,996,291 - -0.6 1,996,307 + -1.0 1,996,307 + +0.3 1,996,307 + -2.0 1,996,307 + -2.3 1,996,307 + -2.0 1,996,307 + +0.1 1,996,312 + +1.2 1,996,312 + +1.0 1,996,322 + -3.3 1,996,356 + -0.9 1,997,252 + asd NIAGMN_10170 0.88 +1.5 1,997,252 + asd NIAGMN_10170 0.88 +0.8 1,997,252 + asd NIAGMN_10170 0.88 -1.1 1,997,257 - asd NIAGMN_10170 0.89 -0.2 1,997,271 + -1.5 1,997,365 + -0.1 1,997,366 - -1.2 1,997,400 + +0.3 1,997,441 - -0.9 1,997,460 + -3.0 1,997,460 + +0.6 1,997,547 - +1.1 1,997,611 - +1.3 1,997,615 - -2.3 1,997,649 - +0.5 1,997,745 + mscM NIAGMN_10175 0.11 -2.0 1,997,745 + mscM NIAGMN_10175 0.11 -1.3 1,997,843 + mscM NIAGMN_10175 0.14 -0.5 1,997,844 - mscM NIAGMN_10175 0.14 +3.1 1,997,844 - mscM NIAGMN_10175 0.14 -3.1 1,997,883 + mscM NIAGMN_10175 0.15 -1.0 1,997,884 - mscM NIAGMN_10175 0.15 +1.2 1,997,884 - mscM NIAGMN_10175 0.15 +1.0 1,997,884 - mscM NIAGMN_10175 0.15 -0.6 1,997,938 - mscM NIAGMN_10175 0.17 -0.0 1,997,938 - mscM NIAGMN_10175 0.17 +0.1 1,997,938 - mscM NIAGMN_10175 0.17 -0.3 1,997,938 - mscM NIAGMN_10175 0.17 -2.1 1,997,996 + mscM NIAGMN_10175 0.18 -0.0 1,997,996 + mscM NIAGMN_10175 0.18 -2.8 1,998,049 + mscM NIAGMN_10175 0.20 -0.0 1,998,049 + mscM NIAGMN_10175 0.20 +1.6 1,998,049 + mscM NIAGMN_10175 0.20 +0.2 1,998,050 - mscM NIAGMN_10175 0.20 +0.9 1,998,126 - mscM NIAGMN_10175 0.22 +1.4 1,998,160 - mscM NIAGMN_10175 0.23 -2.7 1,998,180 + mscM NIAGMN_10175 0.24 -2.2 1,998,291 + mscM NIAGMN_10175 0.27 -0.4 1,998,291 + mscM NIAGMN_10175 0.27 +0.5 1,998,292 - mscM NIAGMN_10175 0.27 +0.4 1,998,292 - mscM NIAGMN_10175 0.27 +0.5
Or see this region's nucleotide sequence