Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnr and nsrR are separated by 38 nucleotides nsrR and purA are separated by 204 nucleotides
NIAGMN_10070: rnr - ribonuclease R, at 1,977,663 to 1,980,104
rnr
NIAGMN_10075: nsrR - nitric oxide-sensing transcriptional repressor NsrR, at 1,980,143 to 1,980,568
nsrR
NIAGMN_10080: purA - adenylosuccinate synthase, at 1,980,773 to 1,982,071
purA
Position (kb)
1980
1981 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 1979.144 kb on + strand, within rnr at 1979.145 kb on - strand, within rnr at 1979.145 kb on - strand, within rnr at 1979.191 kb on + strand, within rnr at 1979.191 kb on + strand, within rnr at 1979.191 kb on + strand, within rnr at 1979.191 kb on + strand, within rnr at 1979.191 kb on + strand, within rnr at 1979.192 kb on - strand, within rnr at 1979.192 kb on - strand, within rnr at 1979.192 kb on - strand, within rnr at 1979.192 kb on - strand, within rnr at 1979.192 kb on - strand, within rnr at 1979.194 kb on + strand, within rnr at 1979.194 kb on + strand, within rnr at 1979.195 kb on - strand, within rnr at 1979.195 kb on - strand, within rnr at 1979.195 kb on - strand, within rnr at 1979.221 kb on - strand, within rnr at 1979.254 kb on + strand, within rnr at 1979.254 kb on + strand, within rnr at 1979.401 kb on + strand, within rnr at 1979.453 kb on + strand, within rnr at 1979.453 kb on + strand, within rnr at 1979.454 kb on - strand, within rnr at 1979.639 kb on - strand, within rnr at 1979.674 kb on - strand, within rnr at 1979.684 kb on + strand, within rnr at 1979.779 kb on + strand, within rnr at 1979.779 kb on + strand, within rnr at 1979.779 kb on + strand, within rnr at 1979.779 kb on + strand, within rnr at 1979.780 kb on - strand, within rnr at 1979.780 kb on - strand, within rnr at 1979.780 kb on - strand, within rnr at 1979.780 kb on - strand, within rnr at 1979.793 kb on - strand, within rnr at 1979.837 kb on + strand, within rnr at 1979.843 kb on + strand, within rnr at 1979.878 kb on + strand at 1979.879 kb on - strand at 1979.894 kb on + strand at 1979.980 kb on - strand at 1980.036 kb on - strand at 1980.048 kb on - strand at 1980.159 kb on - strand at 1980.163 kb on + strand at 1980.204 kb on + strand, within nsrR at 1980.205 kb on - strand, within nsrR at 1980.205 kb on - strand, within nsrR at 1980.205 kb on - strand, within nsrR at 1980.241 kb on + strand, within nsrR at 1980.274 kb on + strand, within nsrR at 1980.274 kb on + strand, within nsrR at 1980.274 kb on + strand, within nsrR at 1980.274 kb on + strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.275 kb on - strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.343 kb on + strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.344 kb on - strand, within nsrR at 1980.348 kb on - strand, within nsrR at 1980.364 kb on + strand, within nsrR at 1980.365 kb on - strand, within nsrR at 1980.421 kb on + strand, within nsrR at 1980.421 kb on + strand, within nsrR at 1980.445 kb on + strand, within nsrR at 1980.446 kb on - strand, within nsrR at 1980.446 kb on - strand, within nsrR at 1980.522 kb on + strand, within nsrR at 1980.522 kb on + strand, within nsrR at 1980.522 kb on + strand, within nsrR at 1980.522 kb on + strand, within nsrR at 1980.523 kb on - strand, within nsrR at 1980.523 kb on - strand, within nsrR at 1980.536 kb on + strand at 1980.561 kb on - strand at 1980.600 kb on - strand at 1980.605 kb on + strand at 1980.605 kb on + strand at 1980.605 kb on + strand at 1980.606 kb on - strand at 1980.606 kb on - strand at 1980.606 kb on - strand at 1980.606 kb on - strand at 1980.607 kb on + strand at 1980.607 kb on + strand at 1980.608 kb on - strand at 1980.608 kb on - strand at 1980.608 kb on - strand at 1980.616 kb on + strand at 1980.617 kb on - strand at 1980.641 kb on - strand at 1980.646 kb on - strand at 1980.646 kb on - strand at 1980.679 kb on + strand at 1980.679 kb on + strand at 1980.680 kb on - strand at 1980.700 kb on - strand at 1980.700 kb on - strand at 1980.725 kb on - strand at 1980.725 kb on - strand at 1980.725 kb on - strand at 1980.725 kb on - strand at 1980.753 kb on - strand at 1980.774 kb on + strand at 1980.775 kb on - strand at 1980.775 kb on - strand at 1980.836 kb on - strand at 1980.877 kb on - strand at 1980.877 kb on - strand at 1980.877 kb on - strand at 1980.955 kb on - strand, within purA at 1980.966 kb on - strand, within purA at 1981.001 kb on - strand, within purA at 1981.078 kb on + strand, within purA at 1981.168 kb on + strand, within purA at 1981.169 kb on - strand, within purA at 1981.263 kb on + strand, within purA at 1981.264 kb on - strand, within purA at 1981.264 kb on - strand, within purA at 1981.288 kb on - strand, within purA at 1981.288 kb on - strand, within purA at 1981.288 kb on - strand, within purA at 1981.296 kb on + strand, within purA at 1981.297 kb on - strand, within purA at 1981.356 kb on - strand, within purA at 1981.356 kb on - strand, within purA at 1981.360 kb on - strand, within purA at 1981.365 kb on + strand, within purA at 1981.365 kb on + strand, within purA at 1981.365 kb on + strand, within purA at 1981.366 kb on - strand, within purA at 1981.366 kb on - strand, within purA at 1981.366 kb on - strand, within purA at 1981.366 kb on - strand, within purA at 1981.366 kb on - strand, within purA at 1981.366 kb on - strand, within purA at 1981.370 kb on - strand, within purA at 1981.394 kb on - strand, within purA at 1981.471 kb on + strand, within purA at 1981.471 kb on + strand, within purA at 1981.472 kb on - strand, within purA at 1981.472 kb on - strand, within purA at 1981.472 kb on - strand, within purA at 1981.472 kb on - strand, within purA at 1981.472 kb on - strand, within purA at 1981.501 kb on + strand, within purA at 1981.502 kb on - strand, within purA at 1981.502 kb on - strand, within purA at 1981.542 kb on + strand, within purA at 1981.542 kb on + strand, within purA at 1981.542 kb on + strand, within purA at 1981.542 kb on + strand, within purA at 1981.543 kb on - strand, within purA at 1981.545 kb on + strand, within purA at 1981.545 kb on + strand, within purA at 1981.545 kb on + strand, within purA at 1981.545 kb on + strand, within purA at 1981.545 kb on + strand, within purA at 1981.546 kb on - strand, within purA at 1981.546 kb on - strand, within purA at 1981.549 kb on + strand, within purA at 1981.550 kb on - strand, within purA at 1981.550 kb on - strand, within purA at 1981.550 kb on - strand, within purA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,979,144 + rnr NIAGMN_10070 0.61 -0.3 1,979,145 - rnr NIAGMN_10070 0.61 +0.9 1,979,145 - rnr NIAGMN_10070 0.61 -1.0 1,979,191 + rnr NIAGMN_10070 0.63 +0.6 1,979,191 + rnr NIAGMN_10070 0.63 +2.0 1,979,191 + rnr NIAGMN_10070 0.63 -2.2 1,979,191 + rnr NIAGMN_10070 0.63 +0.5 1,979,191 + rnr NIAGMN_10070 0.63 -1.1 1,979,192 - rnr NIAGMN_10070 0.63 -4.4 1,979,192 - rnr NIAGMN_10070 0.63 -1.7 1,979,192 - rnr NIAGMN_10070 0.63 +0.2 1,979,192 - rnr NIAGMN_10070 0.63 +0.1 1,979,192 - rnr NIAGMN_10070 0.63 +0.3 1,979,194 + rnr NIAGMN_10070 0.63 +2.5 1,979,194 + rnr NIAGMN_10070 0.63 +0.4 1,979,195 - rnr NIAGMN_10070 0.63 -2.0 1,979,195 - rnr NIAGMN_10070 0.63 +1.1 1,979,195 - rnr NIAGMN_10070 0.63 -0.9 1,979,221 - rnr NIAGMN_10070 0.64 -0.6 1,979,254 + rnr NIAGMN_10070 0.65 -0.3 1,979,254 + rnr NIAGMN_10070 0.65 +0.7 1,979,401 + rnr NIAGMN_10070 0.71 +2.3 1,979,453 + rnr NIAGMN_10070 0.73 +0.1 1,979,453 + rnr NIAGMN_10070 0.73 -0.3 1,979,454 - rnr NIAGMN_10070 0.73 -0.6 1,979,639 - rnr NIAGMN_10070 0.81 +0.6 1,979,674 - rnr NIAGMN_10070 0.82 +4.8 1,979,684 + rnr NIAGMN_10070 0.83 -0.4 1,979,779 + rnr NIAGMN_10070 0.87 -0.5 1,979,779 + rnr NIAGMN_10070 0.87 -1.9 1,979,779 + rnr NIAGMN_10070 0.87 +0.8 1,979,779 + rnr NIAGMN_10070 0.87 -0.4 1,979,780 - rnr NIAGMN_10070 0.87 -3.0 1,979,780 - rnr NIAGMN_10070 0.87 -0.3 1,979,780 - rnr NIAGMN_10070 0.87 -1.9 1,979,780 - rnr NIAGMN_10070 0.87 +1.3 1,979,793 - rnr NIAGMN_10070 0.87 -0.5 1,979,837 + rnr NIAGMN_10070 0.89 +0.7 1,979,843 + rnr NIAGMN_10070 0.89 -1.0 1,979,878 + -1.8 1,979,879 - -3.4 1,979,894 + +1.8 1,979,980 - -1.6 1,980,036 - +0.2 1,980,048 - -1.7 1,980,159 - -0.8 1,980,163 + +0.9 1,980,204 + nsrR NIAGMN_10075 0.14 -1.7 1,980,205 - nsrR NIAGMN_10075 0.15 -0.2 1,980,205 - nsrR NIAGMN_10075 0.15 +0.8 1,980,205 - nsrR NIAGMN_10075 0.15 -2.0 1,980,241 + nsrR NIAGMN_10075 0.23 -1.1 1,980,274 + nsrR NIAGMN_10075 0.31 -2.1 1,980,274 + nsrR NIAGMN_10075 0.31 -1.3 1,980,274 + nsrR NIAGMN_10075 0.31 +0.1 1,980,274 + nsrR NIAGMN_10075 0.31 +2.7 1,980,275 - nsrR NIAGMN_10075 0.31 -1.8 1,980,275 - nsrR NIAGMN_10075 0.31 -1.5 1,980,275 - nsrR NIAGMN_10075 0.31 +1.4 1,980,275 - nsrR NIAGMN_10075 0.31 +1.3 1,980,275 - nsrR NIAGMN_10075 0.31 -1.8 1,980,275 - nsrR NIAGMN_10075 0.31 +2.3 1,980,275 - nsrR NIAGMN_10075 0.31 -1.7 1,980,275 - nsrR NIAGMN_10075 0.31 -1.6 1,980,275 - nsrR NIAGMN_10075 0.31 -0.4 1,980,343 + nsrR NIAGMN_10075 0.47 +1.1 1,980,343 + nsrR NIAGMN_10075 0.47 -2.5 1,980,343 + nsrR NIAGMN_10075 0.47 +0.1 1,980,343 + nsrR NIAGMN_10075 0.47 +0.2 1,980,343 + nsrR NIAGMN_10075 0.47 -0.8 1,980,343 + nsrR NIAGMN_10075 0.47 +1.9 1,980,343 + nsrR NIAGMN_10075 0.47 +0.0 1,980,344 - nsrR NIAGMN_10075 0.47 -1.3 1,980,344 - nsrR NIAGMN_10075 0.47 +1.0 1,980,344 - nsrR NIAGMN_10075 0.47 +0.1 1,980,344 - nsrR NIAGMN_10075 0.47 -0.6 1,980,344 - nsrR NIAGMN_10075 0.47 -1.9 1,980,344 - nsrR NIAGMN_10075 0.47 -1.2 1,980,348 - nsrR NIAGMN_10075 0.48 -1.6 1,980,364 + nsrR NIAGMN_10075 0.52 -2.5 1,980,365 - nsrR NIAGMN_10075 0.52 +1.4 1,980,421 + nsrR NIAGMN_10075 0.65 -2.9 1,980,421 + nsrR NIAGMN_10075 0.65 +1.1 1,980,445 + nsrR NIAGMN_10075 0.71 +0.6 1,980,446 - nsrR NIAGMN_10075 0.71 +0.3 1,980,446 - nsrR NIAGMN_10075 0.71 +0.8 1,980,522 + nsrR NIAGMN_10075 0.89 -3.9 1,980,522 + nsrR NIAGMN_10075 0.89 -0.7 1,980,522 + nsrR NIAGMN_10075 0.89 -2.4 1,980,522 + nsrR NIAGMN_10075 0.89 -2.4 1,980,523 - nsrR NIAGMN_10075 0.89 -2.6 1,980,523 - nsrR NIAGMN_10075 0.89 -0.4 1,980,536 + -2.2 1,980,561 - -2.5 1,980,600 - -2.4 1,980,605 + -1.3 1,980,605 + -1.4 1,980,605 + +1.1 1,980,606 - +0.8 1,980,606 - +0.6 1,980,606 - +0.1 1,980,606 - +2.8 1,980,607 + +1.1 1,980,607 + -0.4 1,980,608 - -0.2 1,980,608 - +0.5 1,980,608 - +1.1 1,980,616 + +1.1 1,980,617 - -0.5 1,980,641 - +1.2 1,980,646 - -0.9 1,980,646 - +0.3 1,980,679 + -0.1 1,980,679 + +0.8 1,980,680 - +0.1 1,980,700 - -0.4 1,980,700 - +1.2 1,980,725 - +0.5 1,980,725 - -0.1 1,980,725 - -1.7 1,980,725 - +0.7 1,980,753 - +0.2 1,980,774 + +0.1 1,980,775 - -1.1 1,980,775 - +0.2 1,980,836 - -3.9 1,980,877 - -4.5 1,980,877 - -3.5 1,980,877 - -3.5 1,980,955 - purA NIAGMN_10080 0.14 -4.1 1,980,966 - purA NIAGMN_10080 0.15 -2.9 1,981,001 - purA NIAGMN_10080 0.18 -4.5 1,981,078 + purA NIAGMN_10080 0.23 -1.3 1,981,168 + purA NIAGMN_10080 0.30 -0.9 1,981,169 - purA NIAGMN_10080 0.30 -1.5 1,981,263 + purA NIAGMN_10080 0.38 -2.5 1,981,264 - purA NIAGMN_10080 0.38 -4.1 1,981,264 - purA NIAGMN_10080 0.38 -4.5 1,981,288 - purA NIAGMN_10080 0.40 -4.6 1,981,288 - purA NIAGMN_10080 0.40 +0.1 1,981,288 - purA NIAGMN_10080 0.40 -2.6 1,981,296 + purA NIAGMN_10080 0.40 -1.6 1,981,297 - purA NIAGMN_10080 0.40 -4.2 1,981,356 - purA NIAGMN_10080 0.45 -3.6 1,981,356 - purA NIAGMN_10080 0.45 -4.3 1,981,360 - purA NIAGMN_10080 0.45 -1.3 1,981,365 + purA NIAGMN_10080 0.46 -1.9 1,981,365 + purA NIAGMN_10080 0.46 +0.1 1,981,365 + purA NIAGMN_10080 0.46 -4.3 1,981,366 - purA NIAGMN_10080 0.46 -3.8 1,981,366 - purA NIAGMN_10080 0.46 -4.3 1,981,366 - purA NIAGMN_10080 0.46 -3.2 1,981,366 - purA NIAGMN_10080 0.46 -3.0 1,981,366 - purA NIAGMN_10080 0.46 -3.6 1,981,366 - purA NIAGMN_10080 0.46 -3.4 1,981,370 - purA NIAGMN_10080 0.46 -3.9 1,981,394 - purA NIAGMN_10080 0.48 -5.2 1,981,471 + purA NIAGMN_10080 0.54 -0.5 1,981,471 + purA NIAGMN_10080 0.54 -2.6 1,981,472 - purA NIAGMN_10080 0.54 -2.5 1,981,472 - purA NIAGMN_10080 0.54 -0.6 1,981,472 - purA NIAGMN_10080 0.54 -5.2 1,981,472 - purA NIAGMN_10080 0.54 -6.3 1,981,472 - purA NIAGMN_10080 0.54 -0.1 1,981,501 + purA NIAGMN_10080 0.56 -1.6 1,981,502 - purA NIAGMN_10080 0.56 -2.1 1,981,502 - purA NIAGMN_10080 0.56 -3.3 1,981,542 + purA NIAGMN_10080 0.59 -5.2 1,981,542 + purA NIAGMN_10080 0.59 -2.2 1,981,542 + purA NIAGMN_10080 0.59 -1.9 1,981,542 + purA NIAGMN_10080 0.59 -1.5 1,981,543 - purA NIAGMN_10080 0.59 -0.6 1,981,545 + purA NIAGMN_10080 0.59 -4.8 1,981,545 + purA NIAGMN_10080 0.59 -2.3 1,981,545 + purA NIAGMN_10080 0.59 -2.6 1,981,545 + purA NIAGMN_10080 0.59 -4.3 1,981,545 + purA NIAGMN_10080 0.59 +1.1 1,981,546 - purA NIAGMN_10080 0.60 -0.9 1,981,546 - purA NIAGMN_10080 0.60 -2.5 1,981,549 + purA NIAGMN_10080 0.60 -4.7 1,981,550 - purA NIAGMN_10080 0.60 -4.6 1,981,550 - purA NIAGMN_10080 0.60 -3.3 1,981,550 - purA NIAGMN_10080 0.60 -0.9
Or see this region's nucleotide sequence