Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfQ and ppa are separated by 309 nucleotides ppa and chpB are separated by 79 nucleotides chpB and chpS are separated by 29 nucleotides chpS and ytfP are separated by 211 nucleotides
NIAGMN_09820: ytfQ - galactofuranose ABC transporter substrate-binding protein YtfQ, at 1,934,086 to 1,935,042
ytfQ
NIAGMN_09825: ppa - inorganic diphosphatase, at 1,935,352 to 1,935,882
ppa
NIAGMN_09830: chpB - type II toxin-antitoxin system ChpB family toxin, at 1,935,962 to 1,936,276
chpB
NIAGMN_09835: chpS - type II toxin-antitoxin system antitoxin ChpS, at 1,936,306 to 1,936,557
chpS
NIAGMN_09840: ytfP - Gamma-glutamylcyclotransferase family protein YtfP, at 1,936,769 to 1,937,110
ytfP
Position (kb)
1935
1936 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1934.393 kb on + strand, within ytfQ at 1934.433 kb on + strand, within ytfQ at 1934.460 kb on + strand, within ytfQ at 1934.503 kb on - strand, within ytfQ at 1934.517 kb on + strand, within ytfQ at 1934.617 kb on + strand, within ytfQ at 1934.617 kb on + strand, within ytfQ at 1934.640 kb on + strand, within ytfQ at 1934.641 kb on - strand, within ytfQ at 1934.676 kb on + strand, within ytfQ at 1934.677 kb on - strand, within ytfQ at 1934.677 kb on - strand, within ytfQ at 1934.677 kb on - strand, within ytfQ at 1934.677 kb on - strand, within ytfQ at 1934.677 kb on - strand, within ytfQ at 1934.678 kb on + strand, within ytfQ at 1934.678 kb on + strand, within ytfQ at 1934.678 kb on + strand, within ytfQ at 1934.756 kb on - strand, within ytfQ at 1934.791 kb on - strand, within ytfQ at 1934.828 kb on - strand, within ytfQ at 1934.828 kb on - strand, within ytfQ at 1934.880 kb on + strand, within ytfQ at 1934.881 kb on - strand, within ytfQ at 1934.992 kb on - strand at 1935.023 kb on - strand at 1935.029 kb on - strand at 1935.042 kb on + strand at 1935.063 kb on - strand at 1935.063 kb on - strand at 1935.067 kb on - strand at 1935.067 kb on - strand at 1935.069 kb on + strand at 1935.069 kb on + strand at 1935.099 kb on - strand at 1935.101 kb on - strand at 1935.109 kb on - strand at 1935.113 kb on - strand at 1935.129 kb on + strand at 1935.130 kb on - strand at 1935.130 kb on - strand at 1935.235 kb on + strand at 1935.305 kb on + strand at 1935.307 kb on + strand at 1935.307 kb on + strand at 1935.310 kb on + strand at 1935.310 kb on + strand at 1935.880 kb on + strand at 1935.880 kb on + strand at 1935.880 kb on + strand at 1935.880 kb on + strand at 1935.895 kb on + strand at 1935.963 kb on + strand at 1935.964 kb on - strand at 1935.986 kb on + strand at 1935.987 kb on - strand at 1935.987 kb on - strand at 1936.028 kb on - strand, within chpB at 1936.049 kb on + strand, within chpB at 1936.112 kb on + strand, within chpB at 1936.113 kb on - strand, within chpB at 1936.113 kb on - strand, within chpB at 1936.113 kb on - strand, within chpB at 1936.113 kb on - strand, within chpB at 1936.193 kb on - strand, within chpB at 1936.313 kb on - strand at 1936.360 kb on - strand, within chpS at 1936.481 kb on - strand, within chpS at 1936.481 kb on - strand, within chpS at 1936.486 kb on - strand, within chpS at 1936.498 kb on + strand, within chpS at 1936.499 kb on - strand, within chpS at 1936.499 kb on - strand, within chpS at 1936.518 kb on + strand, within chpS at 1936.518 kb on + strand, within chpS at 1936.519 kb on - strand, within chpS at 1936.548 kb on + strand at 1936.549 kb on - strand at 1936.549 kb on - strand at 1936.549 kb on - strand at 1936.551 kb on + strand at 1936.551 kb on + strand at 1936.551 kb on + strand at 1936.551 kb on + strand at 1936.552 kb on - strand at 1936.584 kb on - strand at 1936.613 kb on - strand at 1936.658 kb on + strand at 1936.658 kb on + strand at 1936.659 kb on - strand at 1936.659 kb on - strand at 1936.659 kb on - strand at 1936.659 kb on - strand at 1936.659 kb on - strand at 1936.662 kb on + strand at 1936.663 kb on - strand at 1936.663 kb on - strand at 1936.663 kb on - strand at 1936.663 kb on - strand at 1936.704 kb on + strand at 1936.758 kb on + strand at 1936.758 kb on + strand at 1936.759 kb on - strand at 1936.764 kb on + strand at 1936.765 kb on - strand at 1936.770 kb on + strand at 1936.770 kb on + strand at 1936.770 kb on + strand at 1936.771 kb on - strand at 1936.771 kb on - strand at 1936.771 kb on - strand at 1936.771 kb on - strand at 1936.771 kb on - strand at 1936.774 kb on + strand at 1936.774 kb on + strand at 1936.775 kb on - strand at 1936.812 kb on - strand, within ytfP at 1936.812 kb on - strand, within ytfP at 1936.812 kb on - strand, within ytfP at 1936.839 kb on + strand, within ytfP at 1936.840 kb on - strand, within ytfP at 1936.840 kb on - strand, within ytfP at 1936.840 kb on - strand, within ytfP at 1936.840 kb on - strand, within ytfP at 1936.840 kb on - strand, within ytfP at 1936.840 kb on - strand, within ytfP
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,934,393 + ytfQ NIAGMN_09820 0.32 +1.6 1,934,433 + ytfQ NIAGMN_09820 0.36 -3.1 1,934,460 + ytfQ NIAGMN_09820 0.39 +0.7 1,934,503 - ytfQ NIAGMN_09820 0.44 -0.7 1,934,517 + ytfQ NIAGMN_09820 0.45 +1.4 1,934,617 + ytfQ NIAGMN_09820 0.55 +2.0 1,934,617 + ytfQ NIAGMN_09820 0.55 -2.1 1,934,640 + ytfQ NIAGMN_09820 0.58 +1.8 1,934,641 - ytfQ NIAGMN_09820 0.58 -1.4 1,934,676 + ytfQ NIAGMN_09820 0.62 +1.2 1,934,677 - ytfQ NIAGMN_09820 0.62 +2.1 1,934,677 - ytfQ NIAGMN_09820 0.62 -0.5 1,934,677 - ytfQ NIAGMN_09820 0.62 +0.5 1,934,677 - ytfQ NIAGMN_09820 0.62 +0.3 1,934,677 - ytfQ NIAGMN_09820 0.62 +1.8 1,934,678 + ytfQ NIAGMN_09820 0.62 -2.9 1,934,678 + ytfQ NIAGMN_09820 0.62 -2.4 1,934,678 + ytfQ NIAGMN_09820 0.62 +1.4 1,934,756 - ytfQ NIAGMN_09820 0.70 +0.3 1,934,791 - ytfQ NIAGMN_09820 0.74 -1.2 1,934,828 - ytfQ NIAGMN_09820 0.78 -0.2 1,934,828 - ytfQ NIAGMN_09820 0.78 -3.2 1,934,880 + ytfQ NIAGMN_09820 0.83 -1.0 1,934,881 - ytfQ NIAGMN_09820 0.83 +0.9 1,934,992 - -1.9 1,935,023 - -1.8 1,935,029 - -1.8 1,935,042 + +0.7 1,935,063 - +0.3 1,935,063 - +1.8 1,935,067 - -2.4 1,935,067 - +2.5 1,935,069 + -1.7 1,935,069 + -1.0 1,935,099 - +0.5 1,935,101 - +0.9 1,935,109 - -0.2 1,935,113 - +2.9 1,935,129 + -0.0 1,935,130 - +0.5 1,935,130 - +0.6 1,935,235 + -2.3 1,935,305 + -3.0 1,935,307 + +0.7 1,935,307 + +1.3 1,935,310 + -1.1 1,935,310 + -0.4 1,935,880 + +2.3 1,935,880 + +0.5 1,935,880 + +0.1 1,935,880 + +0.1 1,935,895 + +2.9 1,935,963 + +1.0 1,935,964 - +0.3 1,935,986 + +0.9 1,935,987 - -2.5 1,935,987 - -2.2 1,936,028 - chpB NIAGMN_09830 0.21 -1.7 1,936,049 + chpB NIAGMN_09830 0.28 -0.5 1,936,112 + chpB NIAGMN_09830 0.48 -1.0 1,936,113 - chpB NIAGMN_09830 0.48 +1.2 1,936,113 - chpB NIAGMN_09830 0.48 -2.0 1,936,113 - chpB NIAGMN_09830 0.48 -2.1 1,936,113 - chpB NIAGMN_09830 0.48 -0.6 1,936,193 - chpB NIAGMN_09830 0.73 +0.2 1,936,313 - -2.7 1,936,360 - chpS NIAGMN_09835 0.21 +2.2 1,936,481 - chpS NIAGMN_09835 0.69 +1.6 1,936,481 - chpS NIAGMN_09835 0.69 -0.9 1,936,486 - chpS NIAGMN_09835 0.71 -0.6 1,936,498 + chpS NIAGMN_09835 0.76 +1.8 1,936,499 - chpS NIAGMN_09835 0.77 -2.8 1,936,499 - chpS NIAGMN_09835 0.77 +0.4 1,936,518 + chpS NIAGMN_09835 0.84 -1.8 1,936,518 + chpS NIAGMN_09835 0.84 -0.6 1,936,519 - chpS NIAGMN_09835 0.85 +1.1 1,936,548 + -1.7 1,936,549 - -1.2 1,936,549 - +0.3 1,936,549 - -0.3 1,936,551 + -0.2 1,936,551 + +0.8 1,936,551 + -3.2 1,936,551 + +0.7 1,936,552 - +0.2 1,936,584 - -0.1 1,936,613 - -2.2 1,936,658 + +0.8 1,936,658 + +1.5 1,936,659 - +2.0 1,936,659 - +0.2 1,936,659 - +0.0 1,936,659 - -3.3 1,936,659 - +0.2 1,936,662 + -0.5 1,936,663 - +0.1 1,936,663 - +0.1 1,936,663 - +0.9 1,936,663 - +1.9 1,936,704 + +0.7 1,936,758 + -1.6 1,936,758 + -0.9 1,936,759 - -1.3 1,936,764 + -0.7 1,936,765 - -0.5 1,936,770 + -1.6 1,936,770 + -1.8 1,936,770 + +0.0 1,936,771 - -3.5 1,936,771 - -2.5 1,936,771 - +0.6 1,936,771 - +3.6 1,936,771 - -3.2 1,936,774 + +0.7 1,936,774 + +1.9 1,936,775 - -2.1 1,936,812 - ytfP NIAGMN_09840 0.13 -1.0 1,936,812 - ytfP NIAGMN_09840 0.13 +1.6 1,936,812 - ytfP NIAGMN_09840 0.13 +0.8 1,936,839 + ytfP NIAGMN_09840 0.20 -2.2 1,936,840 - ytfP NIAGMN_09840 0.21 +1.5 1,936,840 - ytfP NIAGMN_09840 0.21 -1.4 1,936,840 - ytfP NIAGMN_09840 0.21 +1.4 1,936,840 - ytfP NIAGMN_09840 0.21 -1.0 1,936,840 - ytfP NIAGMN_09840 0.21 +1.5 1,936,840 - ytfP NIAGMN_09840 0.21 -2.8
Or see this region's nucleotide sequence