Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purK and arcC are separated by 138 nucleotides arcC and ylbF overlap by 4 nucleotides ylbF and ylbE are separated by 10 nucleotides
NIAGMN_05895: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,095,994 to 1,097,061
purK
NIAGMN_05900: arcC - carbamate kinase, at 1,097,200 to 1,098,093
arcC
NIAGMN_05905: ylbF - Uncharacterized protein YlbF, at 1,098,090 to 1,098,905
ylbF
NIAGMN_05910: ylbE - Uncharacterized protein YlbE, at 1,098,916 to 1,100,175
ylbE
Position (kb)
1097
1098
1099 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1096.289 kb on + strand, within purK at 1096.289 kb on + strand, within purK at 1096.289 kb on + strand, within purK at 1096.290 kb on - strand, within purK at 1096.290 kb on - strand, within purK at 1096.290 kb on - strand, within purK at 1096.332 kb on - strand, within purK at 1096.350 kb on + strand, within purK at 1096.443 kb on + strand, within purK at 1096.443 kb on + strand, within purK at 1096.443 kb on + strand, within purK at 1096.443 kb on + strand, within purK at 1096.461 kb on - strand, within purK at 1096.536 kb on + strand, within purK at 1096.632 kb on + strand, within purK at 1096.648 kb on + strand, within purK at 1096.725 kb on + strand, within purK at 1096.775 kb on + strand, within purK at 1096.825 kb on - strand, within purK at 1096.867 kb on + strand, within purK at 1096.867 kb on + strand, within purK at 1097.049 kb on + strand at 1097.202 kb on - strand at 1097.278 kb on - strand at 1097.353 kb on + strand, within arcC at 1097.411 kb on + strand, within arcC at 1097.412 kb on - strand, within arcC at 1097.414 kb on - strand, within arcC at 1097.725 kb on - strand, within arcC at 1097.835 kb on + strand, within arcC at 1097.836 kb on - strand, within arcC at 1097.836 kb on - strand, within arcC at 1097.837 kb on + strand, within arcC at 1097.838 kb on - strand, within arcC at 1097.890 kb on + strand, within arcC at 1097.891 kb on - strand, within arcC at 1097.891 kb on - strand, within arcC at 1097.891 kb on - strand, within arcC at 1098.076 kb on + strand at 1098.108 kb on + strand at 1098.109 kb on - strand at 1098.140 kb on + strand at 1098.141 kb on - strand at 1098.141 kb on - strand at 1098.141 kb on - strand at 1098.155 kb on + strand at 1098.155 kb on + strand at 1098.155 kb on + strand at 1098.156 kb on - strand at 1098.156 kb on - strand at 1098.156 kb on - strand at 1098.156 kb on - strand at 1098.156 kb on - strand at 1098.194 kb on + strand, within ylbF at 1098.247 kb on + strand, within ylbF at 1098.267 kb on + strand, within ylbF at 1098.268 kb on - strand, within ylbF at 1098.268 kb on - strand, within ylbF at 1098.349 kb on - strand, within ylbF at 1098.349 kb on - strand, within ylbF at 1098.349 kb on - strand, within ylbF at 1098.358 kb on + strand, within ylbF at 1098.359 kb on - strand, within ylbF at 1098.359 kb on - strand, within ylbF at 1098.359 kb on - strand, within ylbF at 1098.359 kb on - strand, within ylbF at 1098.359 kb on - strand, within ylbF at 1098.362 kb on - strand, within ylbF at 1098.496 kb on - strand, within ylbF at 1098.625 kb on + strand, within ylbF at 1098.625 kb on + strand, within ylbF at 1098.907 kb on - strand at 1099.051 kb on - strand, within ylbE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,096,289 + purK NIAGMN_05895 0.28 -1.9 1,096,289 + purK NIAGMN_05895 0.28 -2.5 1,096,289 + purK NIAGMN_05895 0.28 -4.6 1,096,290 - purK NIAGMN_05895 0.28 -5.2 1,096,290 - purK NIAGMN_05895 0.28 -2.5 1,096,290 - purK NIAGMN_05895 0.28 -1.0 1,096,332 - purK NIAGMN_05895 0.32 -0.2 1,096,350 + purK NIAGMN_05895 0.33 -0.5 1,096,443 + purK NIAGMN_05895 0.42 -1.9 1,096,443 + purK NIAGMN_05895 0.42 -2.6 1,096,443 + purK NIAGMN_05895 0.42 -3.3 1,096,443 + purK NIAGMN_05895 0.42 -1.9 1,096,461 - purK NIAGMN_05895 0.44 -3.8 1,096,536 + purK NIAGMN_05895 0.51 -3.1 1,096,632 + purK NIAGMN_05895 0.60 -2.8 1,096,648 + purK NIAGMN_05895 0.61 -3.4 1,096,725 + purK NIAGMN_05895 0.68 -3.4 1,096,775 + purK NIAGMN_05895 0.73 -2.8 1,096,825 - purK NIAGMN_05895 0.78 -2.6 1,096,867 + purK NIAGMN_05895 0.82 -1.2 1,096,867 + purK NIAGMN_05895 0.82 -2.8 1,097,049 + -2.7 1,097,202 - -0.6 1,097,278 - +1.7 1,097,353 + arcC NIAGMN_05900 0.17 +1.7 1,097,411 + arcC NIAGMN_05900 0.24 -0.5 1,097,412 - arcC NIAGMN_05900 0.24 +2.1 1,097,414 - arcC NIAGMN_05900 0.24 -0.3 1,097,725 - arcC NIAGMN_05900 0.59 -1.6 1,097,835 + arcC NIAGMN_05900 0.71 +2.8 1,097,836 - arcC NIAGMN_05900 0.71 -2.6 1,097,836 - arcC NIAGMN_05900 0.71 -1.1 1,097,837 + arcC NIAGMN_05900 0.71 -0.2 1,097,838 - arcC NIAGMN_05900 0.71 -1.8 1,097,890 + arcC NIAGMN_05900 0.77 +0.8 1,097,891 - arcC NIAGMN_05900 0.77 +1.8 1,097,891 - arcC NIAGMN_05900 0.77 +2.7 1,097,891 - arcC NIAGMN_05900 0.77 -0.8 1,098,076 + +1.9 1,098,108 + +1.2 1,098,109 - -2.1 1,098,140 + -1.0 1,098,141 - +1.5 1,098,141 - -0.5 1,098,141 - -0.4 1,098,155 + +1.1 1,098,155 + -3.6 1,098,155 + +0.3 1,098,156 - +0.1 1,098,156 - +3.0 1,098,156 - -2.6 1,098,156 - +3.1 1,098,156 - +1.0 1,098,194 + ylbF NIAGMN_05905 0.13 -3.3 1,098,247 + ylbF NIAGMN_05905 0.19 +1.1 1,098,267 + ylbF NIAGMN_05905 0.22 +0.4 1,098,268 - ylbF NIAGMN_05905 0.22 -1.2 1,098,268 - ylbF NIAGMN_05905 0.22 -2.3 1,098,349 - ylbF NIAGMN_05905 0.32 +0.8 1,098,349 - ylbF NIAGMN_05905 0.32 -2.3 1,098,349 - ylbF NIAGMN_05905 0.32 +4.2 1,098,358 + ylbF NIAGMN_05905 0.33 -1.1 1,098,359 - ylbF NIAGMN_05905 0.33 +1.0 1,098,359 - ylbF NIAGMN_05905 0.33 -0.5 1,098,359 - ylbF NIAGMN_05905 0.33 -1.0 1,098,359 - ylbF NIAGMN_05905 0.33 +1.0 1,098,359 - ylbF NIAGMN_05905 0.33 -0.3 1,098,362 - ylbF NIAGMN_05905 0.33 +0.6 1,098,496 - ylbF NIAGMN_05905 0.50 +2.3 1,098,625 + ylbF NIAGMN_05905 0.66 -0.2 1,098,625 + ylbF NIAGMN_05905 0.66 +0.0 1,098,907 - -0.9 1,099,051 - ylbE NIAGMN_05910 0.11 -0.4
Or see this region's nucleotide sequence