Strain Fitness in Escherichia coli ECRC102 around NIAGMN_05395

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntleuS and lptE are separated by 14 nucleotideslptE and holA overlap by 1 nucleotidesholA and nadD are separated by 1 nucleotidesnadD and cobC are separated by 23 nucleotides NIAGMN_05385: leuS - leucine--tRNA ligase, at 984,899 to 987,481 leuS NIAGMN_05390: lptE - LPS assembly lipoprotein LptE, at 987,496 to 988,077 lptE NIAGMN_05395: holA - DNA polymerase III subunit delta, at 988,077 to 989,108 holA NIAGMN_05400: nadD - nicotinate-nucleotide adenylyltransferase, at 989,110 to 989,751 nadD NIAGMN_05405: cobC - adenosylcobalamin/alpha-ribazole phosphatase, at 989,775 to 990,386 cobC Position (kb) 988 989 990Strain fitness (log2 ratio) -2 -1 0 1 2at 989.076 kb on - strandat 989.092 kb on + strandat 989.092 kb on + strandat 989.753 kb on - strandat 989.789 kb on - strandat 989.891 kb on + strand, within cobCat 989.892 kb on - strand, within cobCat 989.926 kb on + strand, within cobC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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989,076 - -0.4
989,092 + +1.2
989,092 + -0.5
989,753 - -1.4
989,789 - -0.1
989,891 + cobC NIAGMN_05405 0.19 -0.2
989,892 - cobC NIAGMN_05405 0.19 -2.2
989,926 + cobC NIAGMN_05405 0.25 +2.2

Or see this region's nucleotide sequence