Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides
NIAGMN_04860: tolA - cell envelope integrity protein TolA, at 879,008 to 880,192
tolA
NIAGMN_04865: tolR - colicin uptake protein TolR, at 880,257 to 880,685
tolR
NIAGMN_04870: tolQ - Tol-Pal system protein TolQ, at 880,689 to 881,381
tolQ
NIAGMN_04875: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 881,378 to 881,782
ybgC
NIAGMN_04880: ybgE - cyd operon protein YbgE, at 881,932 to 882,225
ybgE
NIAGMN_04885: cydX - cytochrome bd-I oxidase subunit CydX, at 882,225 to 882,338
cydX
NIAGMN_04890: cydB - cytochrome d ubiquinol oxidase subunit II, at 882,353 to 883,492
cydB
Position (kb) 880
881
882 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 880.002 kb on + strand, within tolA at 880.080 kb on - strand at 880.222 kb on - strand at 880.258 kb on + strand at 880.259 kb on - strand at 880.259 kb on - strand at 880.259 kb on - strand at 880.259 kb on - strand at 880.262 kb on + strand at 880.306 kb on - strand, within tolR at 880.535 kb on + strand, within tolR at 880.965 kb on - strand, within tolQ at 880.966 kb on + strand, within tolQ at 881.186 kb on - strand, within tolQ at 881.401 kb on + strand at 881.402 kb on - strand at 881.479 kb on + strand, within ybgC at 881.497 kb on + strand, within ybgC at 881.498 kb on - strand, within ybgC at 881.498 kb on - strand, within ybgC at 881.561 kb on - strand, within ybgC at 881.584 kb on - strand, within ybgC at 881.584 kb on - strand, within ybgC at 881.603 kb on - strand, within ybgC at 881.603 kb on - strand, within ybgC at 881.701 kb on - strand, within ybgC at 881.701 kb on - strand, within ybgC at 881.701 kb on - strand, within ybgC at 881.713 kb on - strand, within ybgC at 881.713 kb on - strand, within ybgC at 881.742 kb on + strand at 881.742 kb on + strand at 881.743 kb on - strand at 881.743 kb on - strand at 881.746 kb on - strand at 881.746 kb on - strand at 881.776 kb on + strand at 881.776 kb on + strand at 881.777 kb on - strand at 881.785 kb on + strand at 881.785 kb on + strand at 881.786 kb on - strand at 881.786 kb on - strand at 881.829 kb on + strand at 881.829 kb on + strand at 881.830 kb on - strand at 881.833 kb on - strand at 881.833 kb on - strand at 882.071 kb on - strand, within ybgE at 882.071 kb on - strand, within ybgE at 882.133 kb on - strand, within ybgE at 882.133 kb on - strand, within ybgE at 882.133 kb on - strand, within ybgE at 882.133 kb on - strand, within ybgE at 882.200 kb on + strand at 882.201 kb on - strand at 882.201 kb on - strand at 882.220 kb on - strand at 882.351 kb on - strand at 882.351 kb on - strand at 882.351 kb on - strand at 882.351 kb on - strand at 882.355 kb on - strand at 882.355 kb on - strand at 882.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 880,002 + tolA NIAGMN_04860 0.84 +0.3 880,080 - -0.6 880,222 - +2.4 880,258 + -0.5 880,259 - -1.1 880,259 - +0.5 880,259 - +0.9 880,259 - -2.2 880,262 + +2.2 880,306 - tolR NIAGMN_04865 0.11 -1.3 880,535 + tolR NIAGMN_04865 0.65 -1.0 880,965 - tolQ NIAGMN_04870 0.40 +2.2 880,966 + tolQ NIAGMN_04870 0.40 -1.7 881,186 - tolQ NIAGMN_04870 0.72 -0.3 881,401 + +1.4 881,402 - -1.3 881,479 + ybgC NIAGMN_04875 0.25 +0.0 881,497 + ybgC NIAGMN_04875 0.29 +1.3 881,498 - ybgC NIAGMN_04875 0.30 -2.2 881,498 - ybgC NIAGMN_04875 0.30 +0.0 881,561 - ybgC NIAGMN_04875 0.45 +0.6 881,584 - ybgC NIAGMN_04875 0.51 -3.1 881,584 - ybgC NIAGMN_04875 0.51 -0.5 881,603 - ybgC NIAGMN_04875 0.56 +1.7 881,603 - ybgC NIAGMN_04875 0.56 +0.7 881,701 - ybgC NIAGMN_04875 0.80 -1.3 881,701 - ybgC NIAGMN_04875 0.80 +1.6 881,701 - ybgC NIAGMN_04875 0.80 -0.7 881,713 - ybgC NIAGMN_04875 0.83 -1.6 881,713 - ybgC NIAGMN_04875 0.83 -0.3 881,742 + +1.6 881,742 + -1.0 881,743 - -0.3 881,743 - +2.1 881,746 - -0.5 881,746 - +3.3 881,776 + -2.8 881,776 + +1.5 881,777 - -1.2 881,785 + -0.9 881,785 + -2.8 881,786 - +0.1 881,786 - +0.8 881,829 + +0.6 881,829 + +1.8 881,830 - +1.2 881,833 - +0.9 881,833 - +0.1 882,071 - ybgE NIAGMN_04880 0.47 -0.3 882,071 - ybgE NIAGMN_04880 0.47 +1.3 882,133 - ybgE NIAGMN_04880 0.68 +0.4 882,133 - ybgE NIAGMN_04880 0.68 -0.8 882,133 - ybgE NIAGMN_04880 0.68 +1.6 882,133 - ybgE NIAGMN_04880 0.68 +0.3 882,200 + -0.1 882,201 - +0.5 882,201 - +3.0 882,220 - +2.8 882,351 - +0.6 882,351 - -0.5 882,351 - +0.3 882,351 - -1.8 882,355 - -1.5 882,355 - -0.7 882,355 - +3.2
Or see this region's nucleotide sequence