Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_04610 and NIAGMN_04615 are separated by 621 nucleotides NIAGMN_04615 and NIAGMN_04620 are separated by 99 nucleotides NIAGMN_04620 and NIAGMN_04625 overlap by 62 nucleotides NIAGMN_04625 and NIAGMN_04630 are separated by 183 nucleotides
NIAGMN_04610: NIAGMN_04610 - holin, at 839,019 to 839,234
_04610
NIAGMN_04615: NIAGMN_04615 - DUF3927 domain-containing protein, at 839,856 to 839,936
_04615
NIAGMN_04620: NIAGMN_04620 - hypothetical protein, at 840,036 to 840,161
_04620
NIAGMN_04625: NIAGMN_04625 - Membrane protein, at 840,100 to 840,843
_04625
NIAGMN_04630: NIAGMN_04630 - antitermination protein, at 841,027 to 841,716
_04630
Position (kb) 840
841 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 839.045 kb on + strand, within NIAGMN_04610 at 839.284 kb on + strand at 839.285 kb on - strand at 839.317 kb on - strand at 839.317 kb on - strand at 839.319 kb on + strand at 839.319 kb on + strand at 839.355 kb on + strand at 839.381 kb on + strand at 839.381 kb on + strand at 839.385 kb on + strand at 839.385 kb on + strand at 839.385 kb on + strand at 839.479 kb on - strand at 839.513 kb on - strand at 839.536 kb on - strand at 839.707 kb on - strand at 839.714 kb on + strand at 839.715 kb on - strand at 839.764 kb on - strand at 840.050 kb on - strand, within NIAGMN_04620 at 840.050 kb on - strand, within NIAGMN_04620 at 840.936 kb on - strand at 840.936 kb on - strand at 840.936 kb on - strand at 840.942 kb on - strand at 841.018 kb on - strand at 841.120 kb on + strand, within NIAGMN_04630 at 841.156 kb on - strand, within NIAGMN_04630
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 839,045 + NIAGMN_04610 0.12 -0.4 839,284 + -1.0 839,285 - +1.4 839,317 - -2.9 839,317 - +1.2 839,319 + -0.1 839,319 + -0.8 839,355 + +3.0 839,381 + +0.3 839,381 + +0.6 839,385 + +2.3 839,385 + +2.6 839,385 + -0.2 839,479 - -1.9 839,513 - +0.9 839,536 - -2.0 839,707 - -0.9 839,714 + +0.0 839,715 - -0.8 839,764 - +0.8 840,050 - NIAGMN_04620 0.11 +0.3 840,050 - NIAGMN_04620 0.11 -1.2 840,936 - +0.4 840,936 - +1.4 840,936 - -0.8 840,942 - -0.3 841,018 - -1.9 841,120 + NIAGMN_04630 0.13 +0.0 841,156 - NIAGMN_04630 0.19 -0.2
Or see this region's nucleotide sequence