Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrB and bioD are separated by 578 nucleotides bioD and bioC overlap by 8 nucleotides bioC and bioF overlap by 14 nucleotides
NIAGMN_04425: uvrB - excinuclease ABC subunit UvrB, at 803,127 to 805,148
uvrB
NIAGMN_04430: bioD - dethiobiotin synthase, at 805,727 to 806,404
bioD
NIAGMN_04435: bioC - malonyl-ACP O-methyltransferase BioC, at 806,397 to 807,152
bioC
NIAGMN_04440: bioF - 8-amino-7-oxononanoate synthase, at 807,139 to 808,293
bioF
Position (kb) 805
806
807 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 804.794 kb on + strand, within uvrB at 804.795 kb on - strand, within uvrB at 804.795 kb on - strand, within uvrB at 804.844 kb on - strand, within uvrB at 804.848 kb on - strand, within uvrB at 804.848 kb on - strand, within uvrB at 804.848 kb on - strand, within uvrB at 804.848 kb on - strand, within uvrB at 804.862 kb on + strand, within uvrB at 804.865 kb on + strand, within uvrB at 804.866 kb on - strand, within uvrB at 804.871 kb on + strand, within uvrB at 804.928 kb on + strand, within uvrB at 804.964 kb on - strand at 805.182 kb on - strand at 805.186 kb on - strand at 805.208 kb on + strand at 805.254 kb on + strand at 805.254 kb on + strand at 805.255 kb on - strand at 805.255 kb on - strand at 805.315 kb on + strand at 805.435 kb on - strand at 805.536 kb on + strand at 805.536 kb on + strand at 805.537 kb on - strand at 805.558 kb on + strand at 805.728 kb on + strand at 805.729 kb on - strand at 805.841 kb on - strand, within bioD at 805.842 kb on + strand, within bioD at 805.842 kb on + strand, within bioD at 805.843 kb on - strand, within bioD at 805.843 kb on - strand, within bioD at 805.916 kb on + strand, within bioD at 805.916 kb on + strand, within bioD at 805.976 kb on + strand, within bioD at 806.123 kb on + strand, within bioD at 806.123 kb on + strand, within bioD at 806.246 kb on - strand, within bioD at 806.252 kb on + strand, within bioD at 806.252 kb on + strand, within bioD at 806.253 kb on - strand, within bioD at 806.296 kb on - strand, within bioD at 806.334 kb on - strand, within bioD at 806.334 kb on - strand, within bioD at 806.431 kb on + strand at 806.432 kb on - strand at 806.432 kb on - strand at 806.503 kb on - strand, within bioC at 806.587 kb on - strand, within bioC at 806.587 kb on - strand, within bioC at 806.603 kb on + strand, within bioC at 806.614 kb on + strand, within bioC at 806.615 kb on - strand, within bioC at 806.615 kb on - strand, within bioC at 806.797 kb on - strand, within bioC at 806.797 kb on - strand, within bioC at 806.887 kb on + strand, within bioC at 806.887 kb on + strand, within bioC at 806.887 kb on + strand, within bioC at 806.888 kb on - strand, within bioC at 806.888 kb on - strand, within bioC at 806.888 kb on - strand, within bioC at 806.943 kb on + strand, within bioC at 806.943 kb on + strand, within bioC at 806.944 kb on - strand, within bioC at 807.015 kb on + strand, within bioC at 807.016 kb on - strand, within bioC at 807.016 kb on - strand, within bioC at 807.025 kb on + strand, within bioC at 807.030 kb on - strand, within bioC at 807.030 kb on - strand, within bioC at 807.030 kb on - strand, within bioC at 807.051 kb on + strand, within bioC at 807.076 kb on - strand, within bioC at 807.095 kb on - strand at 807.095 kb on - strand at 807.137 kb on + strand at 807.138 kb on - strand at 807.138 kb on - strand at 807.298 kb on + strand, within bioF at 807.361 kb on - strand, within bioF
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 804,794 + uvrB NIAGMN_04425 0.82 +1.4 804,795 - uvrB NIAGMN_04425 0.82 +0.3 804,795 - uvrB NIAGMN_04425 0.82 -0.3 804,844 - uvrB NIAGMN_04425 0.85 -0.5 804,848 - uvrB NIAGMN_04425 0.85 -0.8 804,848 - uvrB NIAGMN_04425 0.85 -0.9 804,848 - uvrB NIAGMN_04425 0.85 +0.2 804,848 - uvrB NIAGMN_04425 0.85 +1.0 804,862 + uvrB NIAGMN_04425 0.86 -0.9 804,865 + uvrB NIAGMN_04425 0.86 -1.5 804,866 - uvrB NIAGMN_04425 0.86 -1.7 804,871 + uvrB NIAGMN_04425 0.86 +0.7 804,928 + uvrB NIAGMN_04425 0.89 +3.1 804,964 - +2.4 805,182 - +1.4 805,186 - -0.3 805,208 + -0.7 805,254 + -0.1 805,254 + -1.0 805,255 - -0.7 805,255 - +0.9 805,315 + +0.3 805,435 - +0.3 805,536 + +0.0 805,536 + -2.5 805,537 - -1.5 805,558 + +0.4 805,728 + -0.3 805,729 - -3.0 805,841 - bioD NIAGMN_04430 0.17 -0.8 805,842 + bioD NIAGMN_04430 0.17 +0.0 805,842 + bioD NIAGMN_04430 0.17 -0.4 805,843 - bioD NIAGMN_04430 0.17 -0.8 805,843 - bioD NIAGMN_04430 0.17 -2.0 805,916 + bioD NIAGMN_04430 0.28 +1.2 805,916 + bioD NIAGMN_04430 0.28 -2.2 805,976 + bioD NIAGMN_04430 0.37 -2.1 806,123 + bioD NIAGMN_04430 0.58 -0.7 806,123 + bioD NIAGMN_04430 0.58 -1.7 806,246 - bioD NIAGMN_04430 0.77 -3.0 806,252 + bioD NIAGMN_04430 0.77 -2.7 806,252 + bioD NIAGMN_04430 0.77 -2.4 806,253 - bioD NIAGMN_04430 0.78 -0.6 806,296 - bioD NIAGMN_04430 0.84 -2.0 806,334 - bioD NIAGMN_04430 0.90 -3.0 806,334 - bioD NIAGMN_04430 0.90 -1.0 806,431 + +1.7 806,432 - -2.6 806,432 - -1.2 806,503 - bioC NIAGMN_04435 0.14 -3.8 806,587 - bioC NIAGMN_04435 0.25 -0.1 806,587 - bioC NIAGMN_04435 0.25 -0.8 806,603 + bioC NIAGMN_04435 0.27 -1.5 806,614 + bioC NIAGMN_04435 0.29 -2.6 806,615 - bioC NIAGMN_04435 0.29 -1.9 806,615 - bioC NIAGMN_04435 0.29 -0.5 806,797 - bioC NIAGMN_04435 0.53 +1.5 806,797 - bioC NIAGMN_04435 0.53 -2.4 806,887 + bioC NIAGMN_04435 0.65 -2.7 806,887 + bioC NIAGMN_04435 0.65 -2.2 806,887 + bioC NIAGMN_04435 0.65 -2.6 806,888 - bioC NIAGMN_04435 0.65 -2.0 806,888 - bioC NIAGMN_04435 0.65 -1.4 806,888 - bioC NIAGMN_04435 0.65 -1.4 806,943 + bioC NIAGMN_04435 0.72 +0.3 806,943 + bioC NIAGMN_04435 0.72 -3.5 806,944 - bioC NIAGMN_04435 0.72 -0.5 807,015 + bioC NIAGMN_04435 0.82 -3.1 807,016 - bioC NIAGMN_04435 0.82 -2.3 807,016 - bioC NIAGMN_04435 0.82 +0.0 807,025 + bioC NIAGMN_04435 0.83 -3.0 807,030 - bioC NIAGMN_04435 0.84 -1.5 807,030 - bioC NIAGMN_04435 0.84 -1.8 807,030 - bioC NIAGMN_04435 0.84 -2.4 807,051 + bioC NIAGMN_04435 0.87 -3.1 807,076 - bioC NIAGMN_04435 0.90 -0.1 807,095 - -2.7 807,095 - -4.8 807,137 + -1.2 807,138 - -3.0 807,138 - -2.5 807,298 + bioF NIAGMN_04440 0.14 -2.0 807,361 - bioF NIAGMN_04440 0.19 -1.5
Or see this region's nucleotide sequence