Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_04290 and ybiI are separated by 75 nucleotides ybiI and ybiJ are separated by 264 nucleotides ybiJ and ybiE are separated by 102 nucleotides ybiE and hcxB are separated by 66 nucleotides
NIAGMN_04290: NIAGMN_04290 - PKHD-type hydroxylase YbiX, at 777,385 to 778,068
_04290
NIAGMN_04295: ybiI - C4-type zinc finger protein YbiI, at 778,144 to 778,410
ybiI
NIAGMN_04300: ybiJ - DUF1471 domain-containing protein, at 778,675 to 778,935
ybiJ
NIAGMN_04310: ybiE - Protein YbiE, at 779,038 to 779,097
ybiE
NIAGMN_04315: hcxB - hydroxycarboxylate dehydrogenase HcXB, at 779,164 to 780,249
hcxB
Position (kb) 778
779 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 777.757 kb on + strand, within NIAGMN_04290 at 777.758 kb on - strand, within NIAGMN_04290 at 777.758 kb on - strand, within NIAGMN_04290 at 777.836 kb on - strand, within NIAGMN_04290 at 777.836 kb on - strand, within NIAGMN_04290 at 777.897 kb on + strand, within NIAGMN_04290 at 777.897 kb on + strand, within NIAGMN_04290 at 777.898 kb on - strand, within NIAGMN_04290 at 777.898 kb on - strand, within NIAGMN_04290 at 777.898 kb on - strand, within NIAGMN_04290 at 777.898 kb on - strand, within NIAGMN_04290 at 777.898 kb on - strand, within NIAGMN_04290 at 778.094 kb on + strand at 778.094 kb on + strand at 778.096 kb on + strand at 778.099 kb on + strand at 778.103 kb on + strand at 778.109 kb on + strand at 778.109 kb on + strand at 778.109 kb on + strand at 778.109 kb on + strand at 778.109 kb on + strand at 778.109 kb on + strand at 778.110 kb on - strand at 778.113 kb on + strand at 778.115 kb on + strand at 778.115 kb on + strand at 778.115 kb on + strand at 778.127 kb on + strand at 778.129 kb on + strand at 778.129 kb on + strand at 778.129 kb on + strand at 778.129 kb on + strand at 778.129 kb on + strand at 778.130 kb on - strand at 778.130 kb on - strand at 778.320 kb on - strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.323 kb on + strand, within ybiI at 778.363 kb on + strand, within ybiI at 778.363 kb on + strand, within ybiI at 778.366 kb on - strand, within ybiI at 778.408 kb on + strand at 778.412 kb on + strand at 778.412 kb on + strand at 778.412 kb on + strand at 778.428 kb on + strand at 778.428 kb on + strand at 778.539 kb on + strand at 778.645 kb on + strand at 778.646 kb on - strand at 778.646 kb on - strand at 778.647 kb on + strand at 778.647 kb on + strand at 778.670 kb on + strand at 778.670 kb on + strand at 778.670 kb on + strand at 778.683 kb on + strand at 778.689 kb on + strand at 778.875 kb on + strand, within ybiJ at 778.875 kb on + strand, within ybiJ at 778.875 kb on + strand, within ybiJ at 778.876 kb on - strand, within ybiJ at 778.902 kb on + strand, within ybiJ at 778.902 kb on + strand, within ybiJ at 778.927 kb on + strand at 778.927 kb on + strand at 778.927 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 778.933 kb on + strand at 779.005 kb on - strand at 779.005 kb on - strand at 779.013 kb on + strand at 779.047 kb on + strand, within ybiE at 779.047 kb on + strand, within ybiE at 779.098 kb on - strand at 779.120 kb on + strand at 779.120 kb on + strand at 779.120 kb on + strand at 779.121 kb on - strand at 779.121 kb on - strand at 779.125 kb on + strand at 779.125 kb on + strand at 779.389 kb on - strand, within hcxB at 779.389 kb on - strand, within hcxB at 779.443 kb on - strand, within hcxB at 779.468 kb on - strand, within hcxB at 779.662 kb on - strand, within hcxB at 779.737 kb on - strand, within hcxB at 779.881 kb on - strand, within hcxB at 779.922 kb on + strand, within hcxB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 777,757 + NIAGMN_04290 0.54 +1.2 777,758 - NIAGMN_04290 0.55 -0.6 777,758 - NIAGMN_04290 0.55 +0.3 777,836 - NIAGMN_04290 0.66 -1.4 777,836 - NIAGMN_04290 0.66 -0.9 777,897 + NIAGMN_04290 0.75 -1.2 777,897 + NIAGMN_04290 0.75 -0.5 777,898 - NIAGMN_04290 0.75 +2.1 777,898 - NIAGMN_04290 0.75 -1.9 777,898 - NIAGMN_04290 0.75 +1.2 777,898 - NIAGMN_04290 0.75 +1.7 777,898 - NIAGMN_04290 0.75 -0.9 778,094 + -1.0 778,094 + -2.4 778,096 + +0.5 778,099 + -3.4 778,103 + +2.4 778,109 + +0.7 778,109 + +2.3 778,109 + -0.1 778,109 + -1.7 778,109 + -1.7 778,109 + -2.7 778,110 - -2.7 778,113 + +1.0 778,115 + -3.2 778,115 + -0.5 778,115 + +0.9 778,127 + +0.3 778,129 + -0.1 778,129 + -1.5 778,129 + +2.4 778,129 + +0.6 778,129 + -0.5 778,130 - +0.0 778,130 - +1.9 778,320 - ybiI NIAGMN_04295 0.66 +0.6 778,323 + ybiI NIAGMN_04295 0.67 +1.9 778,323 + ybiI NIAGMN_04295 0.67 -2.3 778,323 + ybiI NIAGMN_04295 0.67 +0.0 778,323 + ybiI NIAGMN_04295 0.67 +0.5 778,363 + ybiI NIAGMN_04295 0.82 -0.2 778,363 + ybiI NIAGMN_04295 0.82 +3.9 778,366 - ybiI NIAGMN_04295 0.83 +3.9 778,408 + -1.8 778,412 + +2.9 778,412 + +1.1 778,412 + +2.5 778,428 + -3.0 778,428 + +3.0 778,539 + +1.8 778,645 + +1.6 778,646 - +1.3 778,646 - -0.1 778,647 + -0.9 778,647 + +3.4 778,670 + +1.0 778,670 + +2.6 778,670 + -1.3 778,683 + -1.7 778,689 + +0.3 778,875 + ybiJ NIAGMN_04300 0.77 +0.3 778,875 + ybiJ NIAGMN_04300 0.77 -0.6 778,875 + ybiJ NIAGMN_04300 0.77 +0.2 778,876 - ybiJ NIAGMN_04300 0.77 +1.2 778,902 + ybiJ NIAGMN_04300 0.87 +0.1 778,902 + ybiJ NIAGMN_04300 0.87 +0.5 778,927 + -1.7 778,927 + +1.1 778,927 + +0.6 778,933 + +0.7 778,933 + +2.2 778,933 + -0.1 778,933 + -1.0 779,005 - -1.9 779,005 - -0.1 779,013 + +2.1 779,047 + ybiE NIAGMN_04310 0.15 +0.7 779,047 + ybiE NIAGMN_04310 0.15 -1.2 779,098 - -1.8 779,120 + +3.1 779,120 + -2.6 779,120 + -3.2 779,121 - -1.8 779,121 - +1.8 779,125 + +3.0 779,125 + -2.5 779,389 - hcxB NIAGMN_04315 0.21 -0.1 779,389 - hcxB NIAGMN_04315 0.21 -3.6 779,443 - hcxB NIAGMN_04315 0.26 -1.0 779,468 - hcxB NIAGMN_04315 0.28 +2.2 779,662 - hcxB NIAGMN_04315 0.46 +0.9 779,737 - hcxB NIAGMN_04315 0.53 -0.6 779,881 - hcxB NIAGMN_04315 0.66 -0.3 779,922 + hcxB NIAGMN_04315 0.70 -1.9
Or see this region's nucleotide sequence