Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt opgE and NIAGMN_04235 are separated by 48 nucleotides NIAGMN_04235 and yliM are separated by 52 nucleotides yliM and rhtA are separated by 234 nucleotides
NIAGMN_04230: opgE - phosphoethanolamine transferase, at 764,137 to 765,720
opgE
NIAGMN_04235: NIAGMN_04235 - Outer membrane protein X, at 765,769 to 766,284
_04235
NIAGMN_04240: yliM - Protein YliM, at 766,337 to 766,402
yliM
NIAGMN_04245: rhtA - threonine/homoserine exporter RhtA, at 766,637 to 767,524
rhtA
Position (kb) 765
766
767 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 764.791 kb on + strand, within opgE at 764.791 kb on + strand, within opgE at 764.903 kb on - strand, within opgE at 765.063 kb on + strand, within opgE at 765.103 kb on + strand, within opgE at 765.104 kb on - strand, within opgE at 765.305 kb on - strand, within opgE at 765.310 kb on + strand, within opgE at 765.313 kb on - strand, within opgE at 765.315 kb on + strand, within opgE at 765.335 kb on + strand, within opgE at 765.529 kb on - strand, within opgE at 765.646 kb on + strand at 765.646 kb on + strand at 765.646 kb on + strand at 765.646 kb on + strand at 765.646 kb on + strand at 765.647 kb on - strand at 765.679 kb on + strand at 765.679 kb on + strand at 765.754 kb on - strand at 765.771 kb on - strand at 765.806 kb on - strand at 765.806 kb on - strand at 765.806 kb on - strand at 765.817 kb on - strand at 765.837 kb on - strand, within NIAGMN_04235 at 765.891 kb on - strand, within NIAGMN_04235 at 765.971 kb on - strand, within NIAGMN_04235 at 765.971 kb on - strand, within NIAGMN_04235 at 765.971 kb on - strand, within NIAGMN_04235 at 765.978 kb on - strand, within NIAGMN_04235 at 765.978 kb on - strand, within NIAGMN_04235 at 765.978 kb on - strand, within NIAGMN_04235 at 766.029 kb on - strand, within NIAGMN_04235 at 766.032 kb on - strand, within NIAGMN_04235 at 766.047 kb on - strand, within NIAGMN_04235 at 766.082 kb on + strand, within NIAGMN_04235 at 766.083 kb on - strand, within NIAGMN_04235 at 766.083 kb on - strand, within NIAGMN_04235 at 766.128 kb on - strand, within NIAGMN_04235 at 766.134 kb on - strand, within NIAGMN_04235 at 766.134 kb on - strand, within NIAGMN_04235 at 766.202 kb on - strand, within NIAGMN_04235 at 766.207 kb on - strand, within NIAGMN_04235 at 766.228 kb on - strand, within NIAGMN_04235 at 766.285 kb on - strand at 766.308 kb on + strand at 766.309 kb on - strand at 766.309 kb on - strand at 766.316 kb on - strand at 766.327 kb on - strand at 766.327 kb on - strand at 766.327 kb on - strand at 766.403 kb on - strand at 766.450 kb on - strand at 766.454 kb on - strand at 766.454 kb on - strand at 766.457 kb on - strand at 766.533 kb on + strand at 766.534 kb on - strand at 766.583 kb on + strand at 766.584 kb on - strand at 766.586 kb on - strand at 766.592 kb on + strand at 766.592 kb on + strand at 766.593 kb on - strand at 766.610 kb on + strand at 766.611 kb on - strand at 766.611 kb on - strand at 766.611 kb on - strand at 766.620 kb on + strand at 766.620 kb on + strand at 766.621 kb on - strand at 766.621 kb on - strand at 766.621 kb on - strand at 766.621 kb on - strand at 766.621 kb on - strand at 766.650 kb on + strand at 766.655 kb on - strand at 766.677 kb on + strand at 766.683 kb on + strand at 766.683 kb on + strand at 766.683 kb on + strand at 766.683 kb on + strand at 766.705 kb on + strand at 766.705 kb on + strand at 766.729 kb on + strand, within rhtA at 766.729 kb on + strand, within rhtA at 766.729 kb on + strand, within rhtA at 766.786 kb on - strand, within rhtA at 766.909 kb on + strand, within rhtA at 766.930 kb on + strand, within rhtA at 767.043 kb on + strand, within rhtA at 767.123 kb on + strand, within rhtA at 767.123 kb on + strand, within rhtA at 767.123 kb on + strand, within rhtA at 767.130 kb on + strand, within rhtA at 767.282 kb on - strand, within rhtA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 764,791 + opgE NIAGMN_04230 0.41 -1.5 764,791 + opgE NIAGMN_04230 0.41 -1.5 764,903 - opgE NIAGMN_04230 0.48 -2.3 765,063 + opgE NIAGMN_04230 0.58 -0.0 765,103 + opgE NIAGMN_04230 0.61 +0.2 765,104 - opgE NIAGMN_04230 0.61 -0.3 765,305 - opgE NIAGMN_04230 0.74 +1.2 765,310 + opgE NIAGMN_04230 0.74 +0.9 765,313 - opgE NIAGMN_04230 0.74 -1.7 765,315 + opgE NIAGMN_04230 0.74 +2.4 765,335 + opgE NIAGMN_04230 0.76 +1.5 765,529 - opgE NIAGMN_04230 0.88 -0.0 765,646 + -0.3 765,646 + -0.8 765,646 + -1.0 765,646 + +2.7 765,646 + -1.2 765,647 - -0.6 765,679 + +1.0 765,679 + -1.7 765,754 - +1.1 765,771 - +0.8 765,806 - +0.5 765,806 - +0.2 765,806 - +0.3 765,817 - -0.1 765,837 - NIAGMN_04235 0.13 +0.6 765,891 - NIAGMN_04235 0.24 +0.3 765,971 - NIAGMN_04235 0.39 +0.1 765,971 - NIAGMN_04235 0.39 +0.3 765,971 - NIAGMN_04235 0.39 +0.7 765,978 - NIAGMN_04235 0.41 +0.3 765,978 - NIAGMN_04235 0.41 -0.5 765,978 - NIAGMN_04235 0.41 -1.3 766,029 - NIAGMN_04235 0.50 +0.1 766,032 - NIAGMN_04235 0.51 -3.4 766,047 - NIAGMN_04235 0.54 +2.6 766,082 + NIAGMN_04235 0.61 +3.0 766,083 - NIAGMN_04235 0.61 +0.8 766,083 - NIAGMN_04235 0.61 -0.1 766,128 - NIAGMN_04235 0.70 +0.7 766,134 - NIAGMN_04235 0.71 +0.8 766,134 - NIAGMN_04235 0.71 +1.4 766,202 - NIAGMN_04235 0.84 -0.7 766,207 - NIAGMN_04235 0.85 -0.0 766,228 - NIAGMN_04235 0.89 +1.1 766,285 - +0.5 766,308 + +0.5 766,309 - +1.0 766,309 - -2.1 766,316 - +1.0 766,327 - +2.9 766,327 - -1.3 766,327 - -0.2 766,403 - -2.0 766,450 - +0.7 766,454 - -0.6 766,454 - +0.8 766,457 - -1.9 766,533 + -1.3 766,534 - -0.3 766,583 + +1.1 766,584 - -2.9 766,586 - +0.1 766,592 + -0.3 766,592 + -2.5 766,593 - -0.2 766,610 + +1.8 766,611 - +0.0 766,611 - +0.1 766,611 - +0.6 766,620 + +0.5 766,620 + +0.1 766,621 - +0.1 766,621 - -1.8 766,621 - +0.6 766,621 - +0.8 766,621 - +0.1 766,650 + +0.5 766,655 - -1.9 766,677 + -1.5 766,683 + -1.5 766,683 + +1.9 766,683 + +1.1 766,683 + +1.3 766,705 + +1.1 766,705 + +3.1 766,729 + rhtA NIAGMN_04245 0.10 +0.1 766,729 + rhtA NIAGMN_04245 0.10 +2.0 766,729 + rhtA NIAGMN_04245 0.10 -0.9 766,786 - rhtA NIAGMN_04245 0.17 +0.5 766,909 + rhtA NIAGMN_04245 0.31 -1.0 766,930 + rhtA NIAGMN_04245 0.33 -0.1 767,043 + rhtA NIAGMN_04245 0.46 +2.4 767,123 + rhtA NIAGMN_04245 0.55 -1.0 767,123 + rhtA NIAGMN_04245 0.55 +0.4 767,123 + rhtA NIAGMN_04245 0.55 +1.3 767,130 + rhtA NIAGMN_04245 0.56 +0.3 767,282 - rhtA NIAGMN_04245 0.73 +2.3
Or see this region's nucleotide sequence