Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ldtB and ybiR are separated by 57 nucleotides ybiR and mntR overlap by 4 nucleotides mntR and mntS are separated by 185 nucleotides
NIAGMN_04210: ldtB - L,D-transpeptidase, at 760,991 to 761,911
ldtB
NIAGMN_04215: ybiR - Inner membrane protein YbiR, at 761,969 to 763,087
ybiR
NIAGMN_04220: mntR - manganese-binding transcriptional regulator MntR, at 763,084 to 763,551
mntR
NIAGMN_04225: mntS - manganase accumulation protein MntS, at 763,737 to 763,865
mntS
Position (kb) 761
762
763
764 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 761.134 kb on + strand, within ldtB at 761.154 kb on - strand, within ldtB at 761.188 kb on + strand, within ldtB at 761.215 kb on + strand, within ldtB at 761.215 kb on + strand, within ldtB at 761.241 kb on + strand, within ldtB at 761.241 kb on + strand, within ldtB at 761.299 kb on + strand, within ldtB at 761.321 kb on + strand, within ldtB at 761.321 kb on + strand, within ldtB at 761.321 kb on + strand, within ldtB at 761.321 kb on + strand, within ldtB at 761.321 kb on + strand, within ldtB at 761.322 kb on - strand, within ldtB at 761.324 kb on + strand, within ldtB at 761.389 kb on + strand, within ldtB at 761.389 kb on + strand, within ldtB at 761.390 kb on - strand, within ldtB at 761.537 kb on + strand, within ldtB at 761.538 kb on - strand, within ldtB at 761.546 kb on + strand, within ldtB at 761.607 kb on + strand, within ldtB at 761.607 kb on + strand, within ldtB at 761.689 kb on + strand, within ldtB at 761.735 kb on + strand, within ldtB at 761.803 kb on + strand, within ldtB at 761.803 kb on + strand, within ldtB at 761.923 kb on + strand at 761.987 kb on - strand at 762.040 kb on - strand at 762.040 kb on - strand at 762.084 kb on - strand, within ybiR at 762.156 kb on - strand, within ybiR at 762.172 kb on - strand, within ybiR at 762.172 kb on - strand, within ybiR at 762.186 kb on - strand, within ybiR at 762.186 kb on - strand, within ybiR at 762.186 kb on - strand, within ybiR at 762.188 kb on - strand, within ybiR at 762.305 kb on + strand, within ybiR at 762.306 kb on - strand, within ybiR at 762.320 kb on - strand, within ybiR at 762.332 kb on - strand, within ybiR at 762.332 kb on - strand, within ybiR at 762.332 kb on - strand, within ybiR at 762.459 kb on + strand, within ybiR at 762.459 kb on + strand, within ybiR at 762.460 kb on - strand, within ybiR at 762.460 kb on - strand, within ybiR at 762.460 kb on - strand, within ybiR at 762.646 kb on + strand, within ybiR at 762.647 kb on - strand, within ybiR at 762.653 kb on - strand, within ybiR at 762.653 kb on - strand, within ybiR at 762.761 kb on - strand, within ybiR at 762.779 kb on - strand, within ybiR at 762.945 kb on - strand, within ybiR at 763.021 kb on + strand at 763.038 kb on - strand at 763.312 kb on - strand, within mntR at 763.431 kb on - strand, within mntR at 763.431 kb on - strand, within mntR at 763.431 kb on - strand, within mntR at 763.505 kb on - strand at 763.577 kb on + strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.578 kb on - strand at 763.581 kb on + strand at 763.582 kb on - strand at 763.582 kb on - strand at 763.582 kb on - strand at 763.651 kb on + strand at 763.657 kb on + strand at 763.659 kb on + strand at 763.660 kb on - strand at 763.664 kb on + strand at 763.664 kb on + strand at 763.676 kb on + strand at 763.676 kb on + strand at 763.676 kb on + strand at 763.682 kb on + strand at 763.682 kb on + strand at 763.682 kb on + strand at 763.686 kb on + strand at 763.686 kb on + strand at 763.686 kb on + strand at 763.718 kb on + strand at 763.737 kb on - strand at 763.737 kb on - strand at 763.757 kb on + strand, within mntS at 763.789 kb on + strand, within mntS at 763.808 kb on + strand, within mntS at 763.808 kb on + strand, within mntS at 763.983 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 761,134 + ldtB NIAGMN_04210 0.16 -1.3 761,154 - ldtB NIAGMN_04210 0.18 +0.3 761,188 + ldtB NIAGMN_04210 0.21 -0.4 761,215 + ldtB NIAGMN_04210 0.24 -0.3 761,215 + ldtB NIAGMN_04210 0.24 +2.4 761,241 + ldtB NIAGMN_04210 0.27 +1.3 761,241 + ldtB NIAGMN_04210 0.27 -0.4 761,299 + ldtB NIAGMN_04210 0.33 +0.9 761,321 + ldtB NIAGMN_04210 0.36 -1.0 761,321 + ldtB NIAGMN_04210 0.36 +1.4 761,321 + ldtB NIAGMN_04210 0.36 +0.6 761,321 + ldtB NIAGMN_04210 0.36 -1.7 761,321 + ldtB NIAGMN_04210 0.36 -1.5 761,322 - ldtB NIAGMN_04210 0.36 -0.9 761,324 + ldtB NIAGMN_04210 0.36 +1.3 761,389 + ldtB NIAGMN_04210 0.43 +0.7 761,389 + ldtB NIAGMN_04210 0.43 -0.6 761,390 - ldtB NIAGMN_04210 0.43 -0.5 761,537 + ldtB NIAGMN_04210 0.59 +1.9 761,538 - ldtB NIAGMN_04210 0.59 -0.1 761,546 + ldtB NIAGMN_04210 0.60 -1.7 761,607 + ldtB NIAGMN_04210 0.67 -1.4 761,607 + ldtB NIAGMN_04210 0.67 -1.2 761,689 + ldtB NIAGMN_04210 0.76 +1.7 761,735 + ldtB NIAGMN_04210 0.81 +2.7 761,803 + ldtB NIAGMN_04210 0.88 +1.7 761,803 + ldtB NIAGMN_04210 0.88 -1.8 761,923 + +0.2 761,987 - -0.2 762,040 - +1.4 762,040 - +0.5 762,084 - ybiR NIAGMN_04215 0.10 -3.0 762,156 - ybiR NIAGMN_04215 0.17 +3.3 762,172 - ybiR NIAGMN_04215 0.18 +0.7 762,172 - ybiR NIAGMN_04215 0.18 +1.2 762,186 - ybiR NIAGMN_04215 0.19 -2.5 762,186 - ybiR NIAGMN_04215 0.19 +1.1 762,186 - ybiR NIAGMN_04215 0.19 +2.2 762,188 - ybiR NIAGMN_04215 0.20 +0.9 762,305 + ybiR NIAGMN_04215 0.30 -1.3 762,306 - ybiR NIAGMN_04215 0.30 +1.4 762,320 - ybiR NIAGMN_04215 0.31 -2.3 762,332 - ybiR NIAGMN_04215 0.32 -1.2 762,332 - ybiR NIAGMN_04215 0.32 -0.0 762,332 - ybiR NIAGMN_04215 0.32 -0.2 762,459 + ybiR NIAGMN_04215 0.44 -1.3 762,459 + ybiR NIAGMN_04215 0.44 -0.9 762,460 - ybiR NIAGMN_04215 0.44 +0.0 762,460 - ybiR NIAGMN_04215 0.44 -1.9 762,460 - ybiR NIAGMN_04215 0.44 -1.0 762,646 + ybiR NIAGMN_04215 0.61 +1.9 762,647 - ybiR NIAGMN_04215 0.61 -0.6 762,653 - ybiR NIAGMN_04215 0.61 +0.1 762,653 - ybiR NIAGMN_04215 0.61 +0.6 762,761 - ybiR NIAGMN_04215 0.71 -0.4 762,779 - ybiR NIAGMN_04215 0.72 +0.6 762,945 - ybiR NIAGMN_04215 0.87 +1.7 763,021 + +0.4 763,038 - +0.2 763,312 - mntR NIAGMN_04220 0.49 -0.8 763,431 - mntR NIAGMN_04220 0.74 +1.9 763,431 - mntR NIAGMN_04220 0.74 -2.0 763,431 - mntR NIAGMN_04220 0.74 +0.8 763,505 - +0.1 763,577 + +0.2 763,578 - -0.3 763,578 - +0.9 763,578 - -0.9 763,578 - -3.2 763,578 - -1.7 763,578 - -0.4 763,578 - +3.7 763,578 - -0.7 763,581 + +0.3 763,582 - -0.7 763,582 - +1.1 763,582 - -1.5 763,651 + +0.5 763,657 + +2.2 763,659 + +1.9 763,660 - +2.2 763,664 + -1.2 763,664 + +4.2 763,676 + -1.3 763,676 + -0.6 763,676 + +1.0 763,682 + +1.3 763,682 + -3.5 763,682 + -1.8 763,686 + -1.1 763,686 + -0.0 763,686 + +0.5 763,718 + +0.9 763,737 - +1.7 763,737 - +0.3 763,757 + mntS NIAGMN_04225 0.16 -0.5 763,789 + mntS NIAGMN_04225 0.40 -1.4 763,808 + mntS NIAGMN_04225 0.55 +2.3 763,808 + mntS NIAGMN_04225 0.55 -2.9 763,983 + +1.7
Or see this region's nucleotide sequence