Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmC and ybjO are separated by 40 nucleotides ybjO and potI are separated by 59 nucleotides potI and potH overlap by 4 nucleotides potH and potG are separated by 9 nucleotides
NIAGMN_03980: rlmC - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 716,108 to 717,235
rlmC
NIAGMN_03985: ybjO - Inner membrane protein YbjO, at 717,276 to 717,764
ybjO
NIAGMN_03990: potI - putrescine ABC transporter permease PotI, at 717,824 to 718,669
potI
NIAGMN_03995: potH - putrescine ABC transporter permease PotH, at 718,666 to 719,619
potH
NIAGMN_04000: potG - putrescine ABC transporter ATP-binding subunit PotG, at 719,629 to 720,762
potG
Position (kb) 717
718
719 Strain fitness (log2 ratio) -2
-1
0
1
2 at 716.899 kb on - strand, within rlmC at 717.235 kb on + strand at 717.236 kb on - strand at 717.270 kb on + strand at 717.271 kb on - strand at 717.271 kb on - strand at 717.271 kb on - strand at 717.271 kb on - strand at 717.570 kb on + strand, within ybjO at 717.586 kb on - strand, within ybjO at 717.629 kb on + strand, within ybjO at 717.793 kb on + strand at 717.793 kb on + strand at 717.793 kb on + strand at 717.794 kb on - strand at 717.794 kb on - strand at 717.794 kb on - strand at 717.935 kb on + strand, within potI at 717.936 kb on - strand, within potI at 718.246 kb on + strand, within potI at 718.506 kb on - strand, within potI at 718.577 kb on - strand, within potI at 718.799 kb on - strand, within potH at 718.799 kb on - strand, within potH at 718.897 kb on - strand, within potH at 718.897 kb on - strand, within potH at 719.008 kb on - strand, within potH at 719.036 kb on + strand, within potH at 719.043 kb on - strand, within potH at 719.056 kb on - strand, within potH at 719.074 kb on - strand, within potH at 719.210 kb on - strand, within potH at 719.210 kb on - strand, within potH at 719.220 kb on + strand, within potH at 719.325 kb on - strand, within potH at 719.377 kb on - strand, within potH at 719.433 kb on + strand, within potH at 719.458 kb on + strand, within potH at 719.508 kb on + strand, within potH at 719.511 kb on - strand, within potH at 719.630 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 716,899 - rlmC NIAGMN_03980 0.70 -1.1 717,235 + -0.3 717,236 - +0.1 717,270 + +1.1 717,271 - -1.6 717,271 - -1.1 717,271 - +0.1 717,271 - -1.3 717,570 + ybjO NIAGMN_03985 0.60 -0.6 717,586 - ybjO NIAGMN_03985 0.63 -2.3 717,629 + ybjO NIAGMN_03985 0.72 +2.3 717,793 + +0.9 717,793 + +1.4 717,793 + -1.1 717,794 - +1.9 717,794 - +0.6 717,794 - -0.3 717,935 + potI NIAGMN_03990 0.13 +1.4 717,936 - potI NIAGMN_03990 0.13 +0.9 718,246 + potI NIAGMN_03990 0.50 +1.1 718,506 - potI NIAGMN_03990 0.81 +1.3 718,577 - potI NIAGMN_03990 0.89 -0.6 718,799 - potH NIAGMN_03995 0.14 +0.5 718,799 - potH NIAGMN_03995 0.14 -2.3 718,897 - potH NIAGMN_03995 0.24 -1.2 718,897 - potH NIAGMN_03995 0.24 +0.5 719,008 - potH NIAGMN_03995 0.36 +0.4 719,036 + potH NIAGMN_03995 0.39 +0.6 719,043 - potH NIAGMN_03995 0.40 +0.6 719,056 - potH NIAGMN_03995 0.41 -0.1 719,074 - potH NIAGMN_03995 0.43 -0.6 719,210 - potH NIAGMN_03995 0.57 +2.6 719,210 - potH NIAGMN_03995 0.57 +0.4 719,220 + potH NIAGMN_03995 0.58 +1.7 719,325 - potH NIAGMN_03995 0.69 -0.6 719,377 - potH NIAGMN_03995 0.75 +0.5 719,433 + potH NIAGMN_03995 0.80 -1.5 719,458 + potH NIAGMN_03995 0.83 -0.9 719,508 + potH NIAGMN_03995 0.88 -0.9 719,511 - potH NIAGMN_03995 0.89 +1.6 719,630 + +0.2
Or see this region's nucleotide sequence