Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_03950 and artJ are separated by 153 nucleotides artJ and aslA are separated by 50 nucleotides aslA and sgaB are separated by 11 nucleotides sgaB and ulaA are separated by 21 nucleotides
NIAGMN_03950: NIAGMN_03950 - hypothetical protein, at 711,577 to 711,714
_03950
NIAGMN_03960: artJ - ABC transporter substrate-binding protein ArtJ, at 711,868 to 712,599
artJ
NIAGMN_03965: aslA - sulfatase, at 712,650 to 714,131
aslA
NIAGMN_03970: sgaB - PTS sugar transporter IIB, at 714,143 to 714,463
sgaB
NIAGMN_03975: ulaA - PTS sugar transporter subunit IIC, at 714,485 to 715,810
ulaA
Position (kb) 712
713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 711.836 kb on + strand at 711.837 kb on - strand at 712.177 kb on + strand, within artJ at 712.219 kb on + strand, within artJ at 712.220 kb on - strand, within artJ at 712.272 kb on + strand, within artJ at 712.285 kb on + strand, within artJ at 712.379 kb on + strand, within artJ at 712.564 kb on + strand at 712.565 kb on - strand at 712.601 kb on + strand at 712.652 kb on - strand at 712.652 kb on - strand at 712.652 kb on - strand at 712.846 kb on - strand, within aslA at 712.992 kb on - strand, within aslA at 712.992 kb on - strand, within aslA at 712.992 kb on - strand, within aslA at 712.992 kb on - strand, within aslA at 712.994 kb on - strand, within aslA at 712.994 kb on - strand, within aslA at 713.013 kb on + strand, within aslA at 713.014 kb on - strand, within aslA at 713.194 kb on - strand, within aslA at 713.194 kb on - strand, within aslA at 713.246 kb on - strand, within aslA at 713.246 kb on - strand, within aslA at 713.325 kb on - strand, within aslA at 713.325 kb on - strand, within aslA at 713.495 kb on - strand, within aslA at 713.495 kb on - strand, within aslA at 713.507 kb on - strand, within aslA at 713.619 kb on - strand, within aslA at 713.648 kb on + strand, within aslA at 713.840 kb on - strand, within aslA at 713.869 kb on + strand, within aslA at 713.886 kb on - strand, within aslA at 713.949 kb on - strand, within aslA at 713.985 kb on - strand at 713.985 kb on - strand at 713.985 kb on - strand at 713.994 kb on - strand at 714.140 kb on - strand at 714.406 kb on + strand, within sgaB at 714.898 kb on + strand, within ulaA at 714.976 kb on + strand, within ulaA at 714.977 kb on - strand, within ulaA at 714.977 kb on - strand, within ulaA at 714.979 kb on - strand, within ulaA at 714.979 kb on - strand, within ulaA at 714.979 kb on - strand, within ulaA at 715.011 kb on + strand, within ulaA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 711,836 + -1.6 711,837 - +2.4 712,177 + artJ NIAGMN_03960 0.42 -0.6 712,219 + artJ NIAGMN_03960 0.48 -0.5 712,220 - artJ NIAGMN_03960 0.48 +0.9 712,272 + artJ NIAGMN_03960 0.55 -1.0 712,285 + artJ NIAGMN_03960 0.57 -0.9 712,379 + artJ NIAGMN_03960 0.70 +2.3 712,564 + -0.8 712,565 - -0.2 712,601 + +1.0 712,652 - -0.5 712,652 - +2.0 712,652 - +2.3 712,846 - aslA NIAGMN_03965 0.13 -2.2 712,992 - aslA NIAGMN_03965 0.23 -0.3 712,992 - aslA NIAGMN_03965 0.23 +0.3 712,992 - aslA NIAGMN_03965 0.23 -0.7 712,992 - aslA NIAGMN_03965 0.23 +1.2 712,994 - aslA NIAGMN_03965 0.23 +1.3 712,994 - aslA NIAGMN_03965 0.23 -0.7 713,013 + aslA NIAGMN_03965 0.24 -0.8 713,014 - aslA NIAGMN_03965 0.25 -3.5 713,194 - aslA NIAGMN_03965 0.37 -2.0 713,194 - aslA NIAGMN_03965 0.37 -1.2 713,246 - aslA NIAGMN_03965 0.40 +1.6 713,246 - aslA NIAGMN_03965 0.40 +0.5 713,325 - aslA NIAGMN_03965 0.46 -2.1 713,325 - aslA NIAGMN_03965 0.46 -2.0 713,495 - aslA NIAGMN_03965 0.57 +0.5 713,495 - aslA NIAGMN_03965 0.57 +2.3 713,507 - aslA NIAGMN_03965 0.58 +0.9 713,619 - aslA NIAGMN_03965 0.65 +2.4 713,648 + aslA NIAGMN_03965 0.67 -2.6 713,840 - aslA NIAGMN_03965 0.80 +0.9 713,869 + aslA NIAGMN_03965 0.82 +1.1 713,886 - aslA NIAGMN_03965 0.83 -1.6 713,949 - aslA NIAGMN_03965 0.88 -0.1 713,985 - -2.7 713,985 - +0.3 713,985 - -0.9 713,994 - +2.3 714,140 - -1.3 714,406 + sgaB NIAGMN_03970 0.82 -0.3 714,898 + ulaA NIAGMN_03975 0.31 -1.1 714,976 + ulaA NIAGMN_03975 0.37 +2.9 714,977 - ulaA NIAGMN_03975 0.37 -1.6 714,977 - ulaA NIAGMN_03975 0.37 +2.8 714,979 - ulaA NIAGMN_03975 0.37 +0.5 714,979 - ulaA NIAGMN_03975 0.37 +0.7 714,979 - ulaA NIAGMN_03975 0.37 +0.1 715,011 + ulaA NIAGMN_03975 0.40 +0.4
Or see this region's nucleotide sequence