Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysO and hcp are separated by 143 nucleotides hcp and hcr are separated by 11 nucleotides
NIAGMN_03870: lysO - L-lysine exporter LysO, at 695,071 to 695,970
lysO
NIAGMN_03875: hcp - hydroxylamine reductase, at 696,114 to 697,766
hcp
NIAGMN_03880: hcr - NADH oxidoreductase, at 697,778 to 698,746
hcr
Position (kb) 696
697
698 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 695.169 kb on - strand, within lysO at 695.182 kb on + strand, within lysO at 695.183 kb on - strand, within lysO at 695.187 kb on + strand, within lysO at 695.188 kb on - strand, within lysO at 695.239 kb on - strand, within lysO at 695.275 kb on - strand, within lysO at 695.288 kb on + strand, within lysO at 695.296 kb on - strand, within lysO at 695.414 kb on + strand, within lysO at 695.415 kb on - strand, within lysO at 695.483 kb on - strand, within lysO at 695.544 kb on - strand, within lysO at 695.606 kb on + strand, within lysO at 695.642 kb on + strand, within lysO at 695.652 kb on + strand, within lysO at 695.878 kb on - strand, within lysO at 695.923 kb on - strand at 695.936 kb on + strand at 695.936 kb on + strand at 695.968 kb on + strand at 696.606 kb on + strand, within hcp at 696.648 kb on - strand, within hcp at 696.651 kb on + strand, within hcp at 696.660 kb on + strand, within hcp at 696.789 kb on + strand, within hcp at 696.790 kb on - strand, within hcp at 696.991 kb on - strand, within hcp at 696.991 kb on - strand, within hcp at 697.081 kb on + strand, within hcp at 697.089 kb on + strand, within hcp at 697.101 kb on - strand, within hcp at 697.204 kb on - strand, within hcp at 697.273 kb on + strand, within hcp at 697.274 kb on - strand, within hcp at 697.353 kb on + strand, within hcp at 697.353 kb on + strand, within hcp at 697.415 kb on + strand, within hcp at 697.416 kb on - strand, within hcp at 697.487 kb on + strand, within hcp at 697.487 kb on + strand, within hcp at 697.497 kb on + strand, within hcp at 697.497 kb on + strand, within hcp at 697.521 kb on - strand, within hcp at 697.829 kb on - strand at 697.901 kb on + strand, within hcr at 697.921 kb on + strand, within hcr at 697.921 kb on + strand, within hcr at 697.922 kb on - strand, within hcr at 697.979 kb on + strand, within hcr at 697.979 kb on + strand, within hcr at 697.981 kb on + strand, within hcr at 697.981 kb on + strand, within hcr at 697.981 kb on + strand, within hcr at 697.982 kb on - strand, within hcr at 698.043 kb on + strand, within hcr at 698.114 kb on - strand, within hcr at 698.213 kb on + strand, within hcr at 698.355 kb on + strand, within hcr at 698.355 kb on + strand, within hcr at 698.356 kb on - strand, within hcr at 698.356 kb on - strand, within hcr at 698.365 kb on + strand, within hcr at 698.366 kb on - strand, within hcr at 698.393 kb on + strand, within hcr at 698.393 kb on + strand, within hcr at 698.393 kb on + strand, within hcr at 698.393 kb on + strand, within hcr at 698.394 kb on - strand, within hcr at 698.470 kb on - strand, within hcr at 698.529 kb on - strand, within hcr at 698.647 kb on + strand, within hcr at 698.647 kb on + strand, within hcr at 698.647 kb on + strand, within hcr at 698.648 kb on - strand, within hcr at 698.659 kb on + strand at 698.660 kb on - strand at 698.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 695,169 - lysO NIAGMN_03870 0.11 -0.6 695,182 + lysO NIAGMN_03870 0.12 -2.0 695,183 - lysO NIAGMN_03870 0.12 -1.5 695,187 + lysO NIAGMN_03870 0.13 +2.3 695,188 - lysO NIAGMN_03870 0.13 +2.2 695,239 - lysO NIAGMN_03870 0.19 +0.5 695,275 - lysO NIAGMN_03870 0.23 +0.8 695,288 + lysO NIAGMN_03870 0.24 +1.5 695,296 - lysO NIAGMN_03870 0.25 +1.8 695,414 + lysO NIAGMN_03870 0.38 -3.0 695,415 - lysO NIAGMN_03870 0.38 +0.0 695,483 - lysO NIAGMN_03870 0.46 +0.5 695,544 - lysO NIAGMN_03870 0.53 +2.3 695,606 + lysO NIAGMN_03870 0.59 +1.1 695,642 + lysO NIAGMN_03870 0.63 +1.8 695,652 + lysO NIAGMN_03870 0.65 -2.8 695,878 - lysO NIAGMN_03870 0.90 -0.2 695,923 - +0.0 695,936 + -0.5 695,936 + -2.0 695,968 + -2.7 696,606 + hcp NIAGMN_03875 0.30 +2.1 696,648 - hcp NIAGMN_03875 0.32 -2.9 696,651 + hcp NIAGMN_03875 0.32 -0.0 696,660 + hcp NIAGMN_03875 0.33 +0.7 696,789 + hcp NIAGMN_03875 0.41 +1.9 696,790 - hcp NIAGMN_03875 0.41 -0.5 696,991 - hcp NIAGMN_03875 0.53 +0.4 696,991 - hcp NIAGMN_03875 0.53 +4.1 697,081 + hcp NIAGMN_03875 0.58 +2.6 697,089 + hcp NIAGMN_03875 0.59 +0.1 697,101 - hcp NIAGMN_03875 0.60 +4.7 697,204 - hcp NIAGMN_03875 0.66 -1.9 697,273 + hcp NIAGMN_03875 0.70 -0.7 697,274 - hcp NIAGMN_03875 0.70 +0.5 697,353 + hcp NIAGMN_03875 0.75 -1.8 697,353 + hcp NIAGMN_03875 0.75 +1.8 697,415 + hcp NIAGMN_03875 0.79 -1.2 697,416 - hcp NIAGMN_03875 0.79 -0.6 697,487 + hcp NIAGMN_03875 0.83 -2.1 697,487 + hcp NIAGMN_03875 0.83 -3.8 697,497 + hcp NIAGMN_03875 0.84 +0.4 697,497 + hcp NIAGMN_03875 0.84 +0.1 697,521 - hcp NIAGMN_03875 0.85 -2.3 697,829 - +0.9 697,901 + hcr NIAGMN_03880 0.13 -1.5 697,921 + hcr NIAGMN_03880 0.15 -0.2 697,921 + hcr NIAGMN_03880 0.15 +1.2 697,922 - hcr NIAGMN_03880 0.15 -2.5 697,979 + hcr NIAGMN_03880 0.21 -1.4 697,979 + hcr NIAGMN_03880 0.21 +3.7 697,981 + hcr NIAGMN_03880 0.21 +1.5 697,981 + hcr NIAGMN_03880 0.21 +0.3 697,981 + hcr NIAGMN_03880 0.21 +2.5 697,982 - hcr NIAGMN_03880 0.21 -0.7 698,043 + hcr NIAGMN_03880 0.27 -0.1 698,114 - hcr NIAGMN_03880 0.35 +2.5 698,213 + hcr NIAGMN_03880 0.45 -0.9 698,355 + hcr NIAGMN_03880 0.60 -1.4 698,355 + hcr NIAGMN_03880 0.60 -2.2 698,356 - hcr NIAGMN_03880 0.60 +2.3 698,356 - hcr NIAGMN_03880 0.60 +1.5 698,365 + hcr NIAGMN_03880 0.61 -0.5 698,366 - hcr NIAGMN_03880 0.61 +2.8 698,393 + hcr NIAGMN_03880 0.63 -0.4 698,393 + hcr NIAGMN_03880 0.63 -2.6 698,393 + hcr NIAGMN_03880 0.63 -1.1 698,393 + hcr NIAGMN_03880 0.63 -2.4 698,394 - hcr NIAGMN_03880 0.64 -0.6 698,470 - hcr NIAGMN_03880 0.71 +2.9 698,529 - hcr NIAGMN_03880 0.78 -1.1 698,647 + hcr NIAGMN_03880 0.90 -1.1 698,647 + hcr NIAGMN_03880 0.90 +0.5 698,647 + hcr NIAGMN_03880 0.90 -2.4 698,648 - hcr NIAGMN_03880 0.90 +1.4 698,659 + -1.5 698,660 - +3.0 698,700 + +0.3
Or see this region's nucleotide sequence