Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpA and clpS are separated by 30 nucleotides clpS and yljB are separated by 98 nucleotides yljB and cspD are separated by 149 nucleotides cspD and macB are separated by 72 nucleotides macB and macA overlap by 4 nucleotides
NIAGMN_03825: clpA - ATP-dependent Clp protease ATP-binding subunit ClpA, at 684,425 to 686,701
clpA
NIAGMN_03830: clpS - ATP-dependent Clp protease adapter ClpS, at 686,732 to 687,052
clpS
NIAGMN_03835: yljB - Protein YljB, at 687,151 to 687,225
yljB
NIAGMN_03840: cspD - cold shock-like protein CspD, at 687,375 to 687,599
cspD
NIAGMN_03845: macB - macrolide ABC transporter ATP-binding protein/permease MacB, at 687,672 to 689,618
macB
NIAGMN_03850: macA - macrolide transporter subunit MacA, at 689,615 to 690,730
macA
Position (kb) 687
688
689
690 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 686.673 kb on - strand at 686.673 kb on - strand at 686.702 kb on - strand at 686.745 kb on - strand at 686.750 kb on - strand at 686.782 kb on - strand, within clpS at 686.889 kb on - strand, within clpS at 686.889 kb on - strand, within clpS at 686.889 kb on - strand, within clpS at 686.896 kb on - strand, within clpS at 686.896 kb on - strand, within clpS at 686.943 kb on + strand, within clpS at 686.944 kb on - strand, within clpS at 686.958 kb on - strand, within clpS at 686.961 kb on - strand, within clpS at 686.971 kb on - strand, within clpS at 686.971 kb on - strand, within clpS at 686.971 kb on - strand, within clpS at 686.971 kb on - strand, within clpS at 686.974 kb on + strand, within clpS at 686.974 kb on + strand, within clpS at 686.975 kb on - strand, within clpS at 686.975 kb on - strand, within clpS at 686.975 kb on - strand, within clpS at 687.112 kb on + strand at 687.113 kb on - strand at 687.130 kb on - strand at 687.130 kb on - strand at 687.142 kb on - strand at 687.145 kb on + strand at 687.146 kb on - strand at 687.146 kb on - strand at 687.150 kb on - strand at 687.150 kb on - strand at 687.226 kb on - strand at 687.226 kb on - strand at 687.226 kb on - strand at 687.268 kb on - strand at 687.268 kb on - strand at 687.282 kb on + strand at 687.283 kb on - strand at 687.288 kb on + strand at 687.288 kb on + strand at 687.332 kb on + strand at 687.333 kb on - strand at 687.342 kb on + strand at 687.342 kb on + strand at 687.343 kb on - strand at 687.343 kb on - strand at 687.348 kb on + strand at 687.348 kb on + strand at 687.348 kb on + strand at 687.349 kb on - strand at 687.349 kb on - strand at 687.392 kb on + strand at 687.393 kb on - strand at 687.393 kb on - strand at 687.465 kb on + strand, within cspD at 687.499 kb on + strand, within cspD at 687.564 kb on - strand, within cspD at 687.581 kb on - strand at 687.597 kb on + strand at 687.618 kb on + strand at 687.619 kb on - strand at 687.619 kb on - strand at 687.625 kb on + strand at 687.626 kb on - strand at 687.626 kb on - strand at 687.662 kb on - strand at 687.694 kb on - strand at 687.694 kb on - strand at 687.694 kb on - strand at 687.739 kb on - strand at 687.841 kb on - strand at 687.841 kb on - strand at 687.937 kb on - strand, within macB at 687.937 kb on - strand, within macB at 687.937 kb on - strand, within macB at 687.937 kb on - strand, within macB at 687.937 kb on - strand, within macB at 687.937 kb on - strand, within macB at 687.989 kb on + strand, within macB at 687.990 kb on - strand, within macB at 687.990 kb on - strand, within macB at 687.990 kb on - strand, within macB at 688.018 kb on - strand, within macB at 688.018 kb on - strand, within macB at 688.018 kb on - strand, within macB at 688.046 kb on + strand, within macB at 688.062 kb on - strand, within macB at 688.062 kb on - strand, within macB at 688.062 kb on - strand, within macB at 688.124 kb on + strand, within macB at 688.125 kb on - strand, within macB at 688.202 kb on + strand, within macB at 688.203 kb on - strand, within macB at 688.253 kb on - strand, within macB at 688.253 kb on - strand, within macB at 688.258 kb on - strand, within macB at 688.278 kb on - strand, within macB at 688.278 kb on - strand, within macB at 688.335 kb on - strand, within macB at 688.374 kb on - strand, within macB at 688.398 kb on - strand, within macB at 688.400 kb on + strand, within macB at 688.400 kb on + strand, within macB at 688.400 kb on + strand, within macB at 688.401 kb on - strand, within macB at 688.401 kb on - strand, within macB at 688.401 kb on - strand, within macB at 688.480 kb on + strand, within macB at 688.481 kb on - strand, within macB at 688.481 kb on - strand, within macB at 688.488 kb on - strand, within macB at 688.539 kb on - strand, within macB at 688.541 kb on - strand, within macB at 688.686 kb on - strand, within macB at 688.689 kb on - strand, within macB at 688.689 kb on - strand, within macB at 688.701 kb on - strand, within macB at 688.773 kb on - strand, within macB at 688.779 kb on + strand, within macB at 688.780 kb on - strand, within macB at 688.780 kb on - strand, within macB at 688.782 kb on - strand, within macB at 688.801 kb on - strand, within macB at 688.806 kb on - strand, within macB at 688.814 kb on + strand, within macB at 688.857 kb on - strand, within macB at 689.034 kb on - strand, within macB at 689.125 kb on - strand, within macB at 689.281 kb on + strand, within macB at 689.282 kb on - strand, within macB at 689.293 kb on - strand, within macB at 689.426 kb on - strand at 689.426 kb on - strand at 689.481 kb on - strand at 689.509 kb on - strand at 689.525 kb on - strand at 689.525 kb on - strand at 689.525 kb on - strand at 689.762 kb on - strand, within macA at 689.839 kb on + strand, within macA at 689.840 kb on - strand, within macA at 689.904 kb on + strand, within macA at 689.914 kb on + strand, within macA at 689.943 kb on - strand, within macA at 689.946 kb on - strand, within macA at 689.946 kb on - strand, within macA at 689.990 kb on + strand, within macA at 689.991 kb on - strand, within macA at 690.030 kb on - strand, within macA at 690.030 kb on - strand, within macA at 690.030 kb on - strand, within macA at 690.030 kb on - strand, within macA at 690.073 kb on + strand, within macA at 690.074 kb on - strand, within macA at 690.346 kb on - strand, within macA at 690.346 kb on - strand, within macA at 690.458 kb on - strand, within macA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 686,673 - +1.2 686,673 - -1.2 686,702 - -2.1 686,745 - +0.4 686,750 - -1.2 686,782 - clpS NIAGMN_03830 0.16 +0.2 686,889 - clpS NIAGMN_03830 0.49 -0.2 686,889 - clpS NIAGMN_03830 0.49 +1.1 686,889 - clpS NIAGMN_03830 0.49 -0.3 686,896 - clpS NIAGMN_03830 0.51 -2.5 686,896 - clpS NIAGMN_03830 0.51 -1.0 686,943 + clpS NIAGMN_03830 0.66 -2.3 686,944 - clpS NIAGMN_03830 0.66 +1.6 686,958 - clpS NIAGMN_03830 0.70 +0.7 686,961 - clpS NIAGMN_03830 0.71 -0.1 686,971 - clpS NIAGMN_03830 0.74 -2.2 686,971 - clpS NIAGMN_03830 0.74 -1.2 686,971 - clpS NIAGMN_03830 0.74 +0.9 686,971 - clpS NIAGMN_03830 0.74 +1.0 686,974 + clpS NIAGMN_03830 0.75 -0.6 686,974 + clpS NIAGMN_03830 0.75 +0.3 686,975 - clpS NIAGMN_03830 0.76 +0.9 686,975 - clpS NIAGMN_03830 0.76 -1.3 686,975 - clpS NIAGMN_03830 0.76 +0.3 687,112 + +1.6 687,113 - -2.5 687,130 - +0.7 687,130 - -2.6 687,142 - +0.2 687,145 + +1.6 687,146 - -0.1 687,146 - -1.5 687,150 - +1.9 687,150 - -1.1 687,226 - -2.0 687,226 - +1.2 687,226 - +1.2 687,268 - -0.4 687,268 - -1.1 687,282 + -1.4 687,283 - -3.7 687,288 + -1.4 687,288 + +0.8 687,332 + +2.1 687,333 - +0.0 687,342 + +2.0 687,342 + +0.0 687,343 - +1.1 687,343 - -0.5 687,348 + -2.0 687,348 + +0.5 687,348 + -4.3 687,349 - -3.2 687,349 - -1.6 687,392 + -0.8 687,393 - -2.9 687,393 - -0.4 687,465 + cspD NIAGMN_03840 0.40 -2.2 687,499 + cspD NIAGMN_03840 0.55 -2.0 687,564 - cspD NIAGMN_03840 0.84 +1.1 687,581 - +1.8 687,597 + +1.5 687,618 + -2.5 687,619 - +0.5 687,619 - -1.3 687,625 + +1.4 687,626 - -1.9 687,626 - +0.5 687,662 - +1.4 687,694 - +2.0 687,694 - +3.9 687,694 - -2.4 687,739 - -1.6 687,841 - +0.7 687,841 - +1.2 687,937 - macB NIAGMN_03845 0.14 -0.4 687,937 - macB NIAGMN_03845 0.14 -1.5 687,937 - macB NIAGMN_03845 0.14 -2.2 687,937 - macB NIAGMN_03845 0.14 +2.1 687,937 - macB NIAGMN_03845 0.14 -0.5 687,937 - macB NIAGMN_03845 0.14 +0.5 687,989 + macB NIAGMN_03845 0.16 -1.6 687,990 - macB NIAGMN_03845 0.16 +0.2 687,990 - macB NIAGMN_03845 0.16 -0.3 687,990 - macB NIAGMN_03845 0.16 +0.6 688,018 - macB NIAGMN_03845 0.18 +0.3 688,018 - macB NIAGMN_03845 0.18 +2.5 688,018 - macB NIAGMN_03845 0.18 +1.1 688,046 + macB NIAGMN_03845 0.19 +1.2 688,062 - macB NIAGMN_03845 0.20 +0.0 688,062 - macB NIAGMN_03845 0.20 -2.7 688,062 - macB NIAGMN_03845 0.20 -2.5 688,124 + macB NIAGMN_03845 0.23 -1.2 688,125 - macB NIAGMN_03845 0.23 -3.4 688,202 + macB NIAGMN_03845 0.27 +0.8 688,203 - macB NIAGMN_03845 0.27 -2.0 688,253 - macB NIAGMN_03845 0.30 -2.1 688,253 - macB NIAGMN_03845 0.30 +2.4 688,258 - macB NIAGMN_03845 0.30 -0.1 688,278 - macB NIAGMN_03845 0.31 +2.0 688,278 - macB NIAGMN_03845 0.31 -0.6 688,335 - macB NIAGMN_03845 0.34 -2.7 688,374 - macB NIAGMN_03845 0.36 -3.0 688,398 - macB NIAGMN_03845 0.37 -2.0 688,400 + macB NIAGMN_03845 0.37 -0.2 688,400 + macB NIAGMN_03845 0.37 -1.4 688,400 + macB NIAGMN_03845 0.37 -2.5 688,401 - macB NIAGMN_03845 0.37 +0.4 688,401 - macB NIAGMN_03845 0.37 -2.4 688,401 - macB NIAGMN_03845 0.37 +1.8 688,480 + macB NIAGMN_03845 0.41 -2.3 688,481 - macB NIAGMN_03845 0.42 +1.7 688,481 - macB NIAGMN_03845 0.42 +1.2 688,488 - macB NIAGMN_03845 0.42 +1.7 688,539 - macB NIAGMN_03845 0.45 -1.0 688,541 - macB NIAGMN_03845 0.45 +1.4 688,686 - macB NIAGMN_03845 0.52 -1.3 688,689 - macB NIAGMN_03845 0.52 +0.0 688,689 - macB NIAGMN_03845 0.52 -1.1 688,701 - macB NIAGMN_03845 0.53 +0.5 688,773 - macB NIAGMN_03845 0.57 +1.8 688,779 + macB NIAGMN_03845 0.57 +0.2 688,780 - macB NIAGMN_03845 0.57 +1.9 688,780 - macB NIAGMN_03845 0.57 -0.9 688,782 - macB NIAGMN_03845 0.57 -1.9 688,801 - macB NIAGMN_03845 0.58 -0.8 688,806 - macB NIAGMN_03845 0.58 -1.3 688,814 + macB NIAGMN_03845 0.59 -0.4 688,857 - macB NIAGMN_03845 0.61 +0.7 689,034 - macB NIAGMN_03845 0.70 +1.1 689,125 - macB NIAGMN_03845 0.75 -1.1 689,281 + macB NIAGMN_03845 0.83 +2.4 689,282 - macB NIAGMN_03845 0.83 -2.8 689,293 - macB NIAGMN_03845 0.83 +0.6 689,426 - -0.2 689,426 - +0.3 689,481 - +0.3 689,509 - +0.5 689,525 - +0.8 689,525 - -1.3 689,525 - +1.1 689,762 - macA NIAGMN_03850 0.13 +3.5 689,839 + macA NIAGMN_03850 0.20 +2.1 689,840 - macA NIAGMN_03850 0.20 +0.2 689,904 + macA NIAGMN_03850 0.26 -2.2 689,914 + macA NIAGMN_03850 0.27 +1.8 689,943 - macA NIAGMN_03850 0.29 +0.8 689,946 - macA NIAGMN_03850 0.30 +0.0 689,946 - macA NIAGMN_03850 0.30 -0.2 689,990 + macA NIAGMN_03850 0.34 +2.6 689,991 - macA NIAGMN_03850 0.34 +0.9 690,030 - macA NIAGMN_03850 0.37 -1.7 690,030 - macA NIAGMN_03850 0.37 -0.3 690,030 - macA NIAGMN_03850 0.37 -1.2 690,030 - macA NIAGMN_03850 0.37 -1.5 690,073 + macA NIAGMN_03850 0.41 +0.8 690,074 - macA NIAGMN_03850 0.41 -2.8 690,346 - macA NIAGMN_03850 0.66 -1.2 690,346 - macA NIAGMN_03850 0.66 +2.1 690,458 - macA NIAGMN_03850 0.76 -1.0
Or see this region's nucleotide sequence