Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ureF and ureE are separated by 0 nucleotides ureE and ureC are separated by 9 nucleotides ureC and ureB overlap by 8 nucleotides ureB and ureA are separated by 8 nucleotides ureA and ureD1 are separated by 90 nucleotides
NIAGMN_03700: ureF - Urease accessory protein UreF, at 659,782 to 660,456
ureF
NIAGMN_03705: ureE - urease accessory protein UreE, at 660,457 to 660,921
ureE
NIAGMN_03710: ureC - urease subunit alpha, at 660,931 to 662,634
ureC
NIAGMN_03720: ureB - urease subunit beta, at 662,627 to 662,947
ureB
NIAGMN_03725: ureA - urease subunit gamma, at 662,956 to 663,258
ureA
NIAGMN_03730: ureD1 - Putative urease accessory UreD-like protein, at 663,349 to 664,047
ureD1
Position (kb) 660
661
662
663 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 660.086 kb on - strand, within ureF at 660.157 kb on - strand, within ureF at 660.204 kb on - strand, within ureF at 660.257 kb on - strand, within ureF at 660.507 kb on - strand, within ureE at 660.619 kb on + strand, within ureE at 660.673 kb on - strand, within ureE at 661.002 kb on + strand at 661.003 kb on - strand at 661.034 kb on - strand at 661.035 kb on + strand at 661.036 kb on - strand at 661.075 kb on + strand at 661.408 kb on - strand, within ureC at 661.408 kb on - strand, within ureC at 661.416 kb on - strand, within ureC at 661.416 kb on - strand, within ureC at 661.520 kb on - strand, within ureC at 661.520 kb on - strand, within ureC at 661.526 kb on - strand, within ureC at 661.723 kb on - strand, within ureC at 661.724 kb on + strand, within ureC at 661.818 kb on + strand, within ureC at 661.827 kb on + strand, within ureC at 661.861 kb on - strand, within ureC at 661.891 kb on - strand, within ureC at 662.109 kb on - strand, within ureC at 662.109 kb on - strand, within ureC at 662.109 kb on - strand, within ureC at 662.140 kb on - strand, within ureC at 662.324 kb on - strand, within ureC at 662.324 kb on - strand, within ureC at 662.324 kb on - strand, within ureC at 662.524 kb on - strand at 662.528 kb on - strand at 662.609 kb on + strand at 662.610 kb on - strand at 662.629 kb on - strand at 662.629 kb on - strand at 662.658 kb on - strand at 662.658 kb on - strand at 662.658 kb on - strand at 662.659 kb on + strand, within ureB at 662.659 kb on + strand, within ureB at 662.660 kb on - strand, within ureB at 662.660 kb on - strand, within ureB at 662.876 kb on - strand, within ureB at 662.878 kb on + strand, within ureB at 662.879 kb on - strand, within ureB at 662.879 kb on - strand, within ureB at 662.879 kb on - strand, within ureB at 662.888 kb on - strand, within ureB at 662.910 kb on - strand, within ureB at 662.910 kb on - strand, within ureB at 662.912 kb on - strand, within ureB at 662.913 kb on + strand, within ureB at 662.914 kb on - strand, within ureB at 662.914 kb on - strand, within ureB at 662.923 kb on + strand at 662.932 kb on - strand at 662.971 kb on - strand at 663.168 kb on + strand, within ureA at 663.169 kb on - strand, within ureA at 663.169 kb on - strand, within ureA at 663.227 kb on - strand, within ureA at 663.337 kb on - strand at 663.367 kb on - strand at 663.367 kb on - strand at 663.526 kb on - strand, within ureD1
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 660,086 - ureF NIAGMN_03700 0.45 +3.8 660,157 - ureF NIAGMN_03700 0.56 -1.3 660,204 - ureF NIAGMN_03700 0.63 -1.8 660,257 - ureF NIAGMN_03700 0.70 -0.5 660,507 - ureE NIAGMN_03705 0.11 -2.4 660,619 + ureE NIAGMN_03705 0.35 -1.2 660,673 - ureE NIAGMN_03705 0.46 +2.5 661,002 + +1.4 661,003 - +0.7 661,034 - +0.5 661,035 + -2.7 661,036 - -1.0 661,075 + -0.2 661,408 - ureC NIAGMN_03710 0.28 -0.6 661,408 - ureC NIAGMN_03710 0.28 +0.8 661,416 - ureC NIAGMN_03710 0.28 +1.5 661,416 - ureC NIAGMN_03710 0.28 +2.6 661,520 - ureC NIAGMN_03710 0.35 -2.1 661,520 - ureC NIAGMN_03710 0.35 -2.3 661,526 - ureC NIAGMN_03710 0.35 -3.5 661,723 - ureC NIAGMN_03710 0.46 +3.2 661,724 + ureC NIAGMN_03710 0.47 +0.0 661,818 + ureC NIAGMN_03710 0.52 -0.2 661,827 + ureC NIAGMN_03710 0.53 +0.3 661,861 - ureC NIAGMN_03710 0.55 -1.6 661,891 - ureC NIAGMN_03710 0.56 -1.0 662,109 - ureC NIAGMN_03710 0.69 -0.5 662,109 - ureC NIAGMN_03710 0.69 +2.2 662,109 - ureC NIAGMN_03710 0.69 -0.1 662,140 - ureC NIAGMN_03710 0.71 -0.0 662,324 - ureC NIAGMN_03710 0.82 -2.2 662,324 - ureC NIAGMN_03710 0.82 -2.2 662,324 - ureC NIAGMN_03710 0.82 -1.9 662,524 - +1.1 662,528 - +1.2 662,609 + -0.5 662,610 - +0.0 662,629 - -0.9 662,629 - -1.4 662,658 - -2.3 662,658 - -0.6 662,658 - +0.3 662,659 + ureB NIAGMN_03720 0.10 -1.6 662,659 + ureB NIAGMN_03720 0.10 -1.8 662,660 - ureB NIAGMN_03720 0.10 +0.5 662,660 - ureB NIAGMN_03720 0.10 +0.7 662,876 - ureB NIAGMN_03720 0.78 +0.4 662,878 + ureB NIAGMN_03720 0.78 -0.2 662,879 - ureB NIAGMN_03720 0.79 -0.4 662,879 - ureB NIAGMN_03720 0.79 +1.0 662,879 - ureB NIAGMN_03720 0.79 -2.3 662,888 - ureB NIAGMN_03720 0.81 -1.6 662,910 - ureB NIAGMN_03720 0.88 +1.2 662,910 - ureB NIAGMN_03720 0.88 +0.3 662,912 - ureB NIAGMN_03720 0.89 +2.0 662,913 + ureB NIAGMN_03720 0.89 +2.4 662,914 - ureB NIAGMN_03720 0.89 +1.5 662,914 - ureB NIAGMN_03720 0.89 -2.9 662,923 + -1.4 662,932 - +2.7 662,971 - -2.9 663,168 + ureA NIAGMN_03725 0.70 +2.0 663,169 - ureA NIAGMN_03725 0.70 -0.5 663,169 - ureA NIAGMN_03725 0.70 -3.3 663,227 - ureA NIAGMN_03725 0.89 -0.4 663,337 - -0.0 663,367 - -1.9 663,367 - -1.7 663,526 - ureD1 NIAGMN_03730 0.25 +2.5
Or see this region's nucleotide sequence