Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhaC and tnp-IS3 are separated by 180 nucleotides tnp-IS3 and NIAGMN_03465 overlap by 1 nucleotides NIAGMN_03465 and NIAGMN_03470 are separated by 593 nucleotides NIAGMN_03470 and NIAGMN_03475 are separated by 52 nucleotides
NIAGMN_03455: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 620,156 to 621,202
fhaC
NIAGMN_03460: tnp-IS3 - IS3 family transposase, at 621,383 to 621,709
tnp-IS3
NIAGMN_03465: NIAGMN_03465 - IS3 family transposase, at 621,709 to 622,596
_03465
NIAGMN_03470: NIAGMN_03470 - DUF4222 domain-containing protein, at 623,190 to 623,381
_03470
NIAGMN_03475: NIAGMN_03475 - Z1636-like protein, at 623,434 to 623,667
_03475
Position (kb) 621
622
623 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 620.737 kb on + strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.789 kb on - strand, within fhaC at 620.789 kb on - strand, within fhaC at 620.854 kb on - strand, within fhaC at 620.854 kb on - strand, within fhaC at 621.041 kb on - strand, within fhaC at 621.180 kb on - strand at 621.192 kb on - strand at 621.314 kb on + strand at 621.314 kb on + strand at 621.315 kb on - strand at 621.315 kb on - strand at 622.655 kb on - strand at 622.935 kb on + strand at 623.409 kb on - strand at 623.433 kb on - strand at 623.433 kb on - strand at 623.436 kb on - strand at 623.487 kb on - strand, within NIAGMN_03475 at 623.519 kb on - strand, within NIAGMN_03475 at 623.519 kb on - strand, within NIAGMN_03475 at 623.519 kb on - strand, within NIAGMN_03475 at 623.521 kb on - strand, within NIAGMN_03475
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 620,737 + fhaC NIAGMN_03455 0.55 +3.9 620,740 - fhaC NIAGMN_03455 0.56 -0.9 620,740 - fhaC NIAGMN_03455 0.56 -0.4 620,740 - fhaC NIAGMN_03455 0.56 -1.8 620,789 - fhaC NIAGMN_03455 0.60 -1.3 620,789 - fhaC NIAGMN_03455 0.60 +0.9 620,854 - fhaC NIAGMN_03455 0.67 -1.6 620,854 - fhaC NIAGMN_03455 0.67 -0.3 621,041 - fhaC NIAGMN_03455 0.85 +1.0 621,180 - +0.2 621,192 - -2.7 621,314 + -1.1 621,314 + -0.4 621,315 - -0.5 621,315 - -2.2 622,655 - -1.0 622,935 + -1.4 623,409 - -1.4 623,433 - -2.0 623,433 - -1.0 623,436 - -1.1 623,487 - NIAGMN_03475 0.23 -2.9 623,519 - NIAGMN_03475 0.36 -1.0 623,519 - NIAGMN_03475 0.36 +1.2 623,519 - NIAGMN_03475 0.36 -2.0 623,521 - NIAGMN_03475 0.37 -0.1
Or see this region's nucleotide sequence